Figure 1.

Binding profiles of DS-SILY₂₀ to PDGF and IFN-γ. Saturation binding of (A) PDGF and (B) IFN-γ to DS-SILY₂₀. Immobilized DS-SILY₂₀ was incubated with increasing amounts of PDGF or IFN-γ. The growth factor binding was determined by using biotin-conjugated rabbit-antihuman PDGF or rabbit-antihuman IFN-γ, followed by streptavidin-HRP colorimetric reaction. Insets show a Scatchard-type plot of the experimental data for PDGF and IFN-γ binding to DS-SILY₂₀. Dissociation constants (K_d) were calculated by Scatchard analysis. PDGF binding to particular GAG was analyzed in five experiments. Results of one representative experiment for each GAG are shown.