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. 1995 Nov 21;92(24):10899–10903. doi: 10.1073/pnas.92.24.10899

Fixing human factor IX (fIX): correction of a cryptic RNA splice enables the production of biologically active fIX in the mammary gland of transgenic mice.

F Yull 1, G Harold 1, R Wallace 1, A Cowper 1, J Percy 1, I Cottingham 1, A J Clark 1
PMCID: PMC40538  PMID: 7479906

Abstract

Transgenic mice and sheep secrete only low levels of human factor IX in their milk because of an aberrant splicing of the transgene RNA in the mammary gland. Removal of the cryptic 3' splice site prevents this splicing and leads to the production of relatively high levels of factor IX. The purified protein is fully active showing that the mammary gland is capable of the efficient post-translational modification of this protein and that transgenic animals are a suitable means of its production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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