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. 2014 Mar 26;5(9):2372–2389. doi: 10.18632/oncotarget.1706

Figure 2. The combination of NVP-AUY922 and NVP-BEZ235 synergistically induced apoptosis in CCA cell lines.

Figure 2

(A) CGCCA and HuCCT1 cells were incubated with various concentrations (0, 8, 16, 32, 64, or 128 nM) of either NVP-AUY922 or NVP-BEZ235 for 72 h; (B) CGCCA and HuCCT1 cells were incubated with either NVP-AUY922 or NVP-BEZ235 or both NVP-AUY922 and NVP-BEZ235 at various concentrations for 72 h. The combination index (CI) < 1, CI = 1, or CI > 1 indicate synergism, an additive effect, or antagonism, respectively; (C) CGCCA and HuCCT1 cells were treated with 0.5 uM of either NVP-AUY922 or NVP-BEZ235 or a combination of these 2 for 72 h. The number of apoptotic cells measured using the TACS Annexin V-FITC apoptosis detection kit is represented as a percentage of the total events. (D) The immunoblots are analyses of cleaved poly (ADP-ribose) polymerase (PARP). β-Actin was used as the loading control.