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. 2014 Apr 18;5(9):2778–2791. doi: 10.18632/oncotarget.1916

Figure 2. (A) Cell cycle analysis of A549 cells harboring anti-IDO shRNA or scrambled control shRNA, treated or untreated with IFN gamma (25 ng/ml, 48 h).

Figure 2

Bars indicate the mean of 2 independently obtained measurements (n=3 for each experiment, total n=6) obtained from each of 3 clones harboring anti-IDO shRNA and 3 clones harboring scrambled control shRNA ± SD (*p ≤ 0.05). (B) NAD+ levels in A549-derived clonal cell lines (with and without anti-IDO shRNA) after induction of IDO for 48 h. The anti-IDO shRNA-transfected clones are statistically significant from the scrambled control shRNA clones. Bars indicate the mean of 2 independent measurements ± SD (*p≤0.05).