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Immunostaining (A and B) and quantitative assessment (C) of the MyoD:EDU double-positive cells (blue arrows) between male WT and SallAR mutants at E15.5 and E16.5 (E and F) are shown. The BC is inside the traced region. Three embryonic specimens from different litters were analyzed by counting cells in the whole BC region (three to four sections per sample). *, P < .05. Error bars, means ± SE.
