Figure 6.

Spontaneous activity of arcuate Tac2-EGFP neurons in OVX and OVX + E₂ mice. A, Representative recordings of neurons classified as either silent (mean firing frequency <0.1 Hz) or active. B, Proportions of silent or active or silent neurons in OVX mice (n = 22 neurons from 12 mice) and OVX + E₂ mice (n = 15 neurons from 11 mice). More active neurons were identified in OVX mice, but this difference was not statistically significant (Fisher's exact test). C, There was no significant difference between OVX and OVX + E₂ mice in the mean spontaneous firing frequency of neurons classified as either spontaneously active or silent. D, Similarly, no difference was detected in the EPSC events per minute between treatment groups; mean ± SEM; n, number of Tac2-EGFP neurons.