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. 2014 May 20;53(23):3851–3857. doi: 10.1021/bi401633w

Figure 4.

Figure 4

Autolytic forms display activity similar to that of full-length HtrA1. The activity of full-length HtrA1 and its autolytic products was tested by incubating β-casein (3 μg) with increasing amounts of HtrA1 (preincubated for 20 h with or without DTT). After 30 min, degradation products of β-casein were easily identified, and no significant difference in the intensity or sizes of the products was observed between full-length HtrA1 and the autolytically processed versions. Assays were also performed after incubation of β-casein with HtrA1 for 45 min or 1 h, giving similar results (data not shown).