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. 1995 Nov 7;92(23):10782–10786. doi: 10.1073/pnas.92.23.10782

Distribution of parthenogenetic cells in the mouse brain and their influence on brain development and behavior.

N D Allen 1, K Logan 1, G Lally 1, D J Drage 1, M L Norris 1, E B Keverne 1
PMCID: PMC40696  PMID: 7479883

Abstract

A systematic analysis of parthenogenetic (PG) cell fate within the central nervous system (CNS) was made throughout fetal development and neonatal and adult life. Chimeras were made between PG embryos carrying a ubiquitously expressed lacZ transgene and normal fertilized embryos. After detailed histological analysis, we find that the developmental potential of PG cells is spatially restricted to certain parts of the brain. PG cells are prevalent in telencephalic structures and are largely excluded from diencephalic structures, especially the hypothalamus. These spatial restrictions are established early in development. Behavioral studies with chimeras identified an increase in male aggression when the proportion of PG cells in the brain was high. These studies demonstrate that imprinted genes play key roles in development of the CNS and may be involved in behavior.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen N. D., Barton S. C., Hilton K., Norris M. L., Surani M. A. A functional analysis of imprinting in parthenogenetic embryonic stem cells. Development. 1994 Jun;120(6):1473–1482. doi: 10.1242/dev.120.6.1473. [DOI] [PubMed] [Google Scholar]
  2. Allen N. D., Keverne E. B., Surani M. A. A position-dependent transgene reveals patterns of gene expression in the developing brain. Brain Res Dev Brain Res. 1990 Sep 1;55(2):181–190. doi: 10.1016/0165-3806(90)90199-9. [DOI] [PubMed] [Google Scholar]
  3. Barton S. C., Surani M. A., Norris M. L. Role of paternal and maternal genomes in mouse development. 1984 Sep 27-Oct 3Nature. 311(5984):374–376. doi: 10.1038/311374a0. [DOI] [PubMed] [Google Scholar]
  4. Cattanach B. M., Beechey C. V. Autosomal and X-chromosome imprinting. Dev Suppl. 1990:63–72. [PubMed] [Google Scholar]
  5. Cattanach B. M., Kirk M. Differential activity of maternally and paternally derived chromosome regions in mice. Nature. 1985 Jun 6;315(6019):496–498. doi: 10.1038/315496a0. [DOI] [PubMed] [Google Scholar]
  6. Chess A., Simon I., Cedar H., Axel R. Allelic inactivation regulates olfactory receptor gene expression. Cell. 1994 Sep 9;78(5):823–834. doi: 10.1016/s0092-8674(94)90562-2. [DOI] [PubMed] [Google Scholar]
  7. Efstratiadis A. Parental imprinting of autosomal mammalian genes. Curr Opin Genet Dev. 1994 Apr;4(2):265–280. doi: 10.1016/s0959-437x(05)80054-1. [DOI] [PubMed] [Google Scholar]
  8. Friedrich G., Soriano P. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 1991 Sep;5(9):1513–1523. doi: 10.1101/gad.5.9.1513. [DOI] [PubMed] [Google Scholar]
  9. Fundele R. H., Surani M. A. Experimental embryological analysis of genetic imprinting in mouse development. Dev Genet. 1994;15(6):515–522. doi: 10.1002/dvg.1020150610. [DOI] [PubMed] [Google Scholar]
  10. Harvey P. H., Krebs J. R. Comparing brains. Science. 1990 Jul 13;249(4965):140–146. doi: 10.1126/science.2196673. [DOI] [PubMed] [Google Scholar]
  11. Henery C. C., Kaufman M. H. The incidence of aneuploidy after single pulse electroactivation of mouse oocytes. Mol Reprod Dev. 1993 Mar;34(3):299–307. doi: 10.1002/mrd.1080340310. [DOI] [PubMed] [Google Scholar]
  12. Martin R. D. Relative brain size and basal metabolic rate in terrestrial vertebrates. Nature. 1981 Sep 3;293(5827):57–60. doi: 10.1038/293057a0. [DOI] [PubMed] [Google Scholar]
  13. McGrath J., Solter D. Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell. 1984 May;37(1):179–183. doi: 10.1016/0092-8674(84)90313-1. [DOI] [PubMed] [Google Scholar]
  14. Nicholls R. D. New insights reveal complex mechanisms involved in genomic imprinting. Am J Hum Genet. 1994 May;54(5):733–740. [PMC free article] [PubMed] [Google Scholar]
  15. Schinzel A. NICHD conference. Genomic imprinting: consequences of uniparental disomy for human disease. Am J Med Genet. 1993 Jul 1;46(6):683–684. doi: 10.1002/ajmg.1320460616. [DOI] [PubMed] [Google Scholar]
  16. Sturm K. S., Flannery M. L., Pedersen R. A. Abnormal development of embryonic and extraembryonic cell lineages in parthenogenetic mouse embryos. Dev Dyn. 1994 Sep;201(1):11–28. doi: 10.1002/aja.1002010103. [DOI] [PubMed] [Google Scholar]

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