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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Bone. 2014 May 2;64:273–280. doi: 10.1016/j.bone.2014.04.028

Figure 3.

Figure 3

Effect of Notch on Ctgf expression in the context of Rbpjκ downregulation. Calvarial osteoblasts isolated from RosaNotch mice were transduced with Ad-CMV-Cre, to activate Notch (black bars), or with control Ad-CMV-GFP (white bars), transfected with Rbpjκ small interfering RNA (siRbpjκ) or scrambled siRNA (siScram) and cultured for 72 h. Total RNA was extracted, reverse transcribed and amplified by qRT-PCR. Data are expressed as copy number of Rbpjκ mRNA, Ctgf mRNA and Ctgf hnRNA, corrected for Rpl38. Values are means ± SEM; n = 4. * Significantly different between siRbpjκ and siScram, p < 0.05.