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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Science. 2013 Nov 29;343(6168):305–309. doi: 10.1126/science.1244917

Fig. 1. Down-regulation of IKZF1 and IKZF3 by lenalidomide.

Fig. 1

(A) Vector schematic. (B) Distribution of fold change in Fluc/Rluc ratios after lenalidomide (LEN) (2 μM) treatment. (C and D) Fluc/Rluc ratios (top panels) and immunoblots (bottom panels) of 293FT cells transfected to produce the indicated IKZF proteins fused to Fluc (top panels) or HA tag (bottom panels). Where indicated, cells were treated with lenalidomide (2 μM), MLN4924 (1 μM), or MG132 (10 μM) for 12 hours. Fluc/Rluc ratios were normalized to corresponding dimethyl sulfoxide (DMSO)–treated cells. Data are presented as mean ± SD (n = 4). (E) Immunoblot analysis of MM1S and L363 cells treated with LEN (2 μM) and MLN4924 (1 μM), as indicated, for 12 hours.