Table 2.
Inoculation | Isolate | Mortality%* | ||||
---|---|---|---|---|---|---|
Galleria mellonella | Musca domestica | Tenebrio molitor | Otiorhynchus sulcatus | |||
Dipping | Geomyces I | 35.0 ± 8.7a | - | - | - | |
Geomyces II | 32.5 ± 4.8a | - | - | - | ||
Mortierella signyensis | 12.6 ± 6.3a | - | - | - | ||
control | 20.0 ± 4.1a | - | - | - | ||
Injection | Geomyces I | 8.3 ± 3.3a | 29.2 ± 6.8a | 10.0 ± 2.4a | - | |
Geomyces II | 1.7 ± 1.2ab | 26.7 ± 6.5a | 8.8 ± 2.0a | - | ||
Mortierella signyensis | 94.2 ± 2.8c | 62.5 ± 7.4b | 67.5 ± 4.1b | - | ||
control | 5.0 ± 3.2ab | 15.0 ± 3.9a | 7.5 ± 2.2a | - | ||
Soil | Mortierella signyensis | 0.4 ± 6.1a | 38.3 ± 3.0a | - | 6.0 ± 3.1a | |
Mortierella alpina | 50.7 ± 9.8b | 33.0 ± 4.4a | - | 2.0 ± 2.0a | ||
control | 0.8 ± 0.6a | 33.7 ± 0.7a | - | 12.0 ± 6.1a | ||
Baiting | food | Mortierella signyensis | - | 34.7 ± 7.1ab | - | - |
Mortierella alpina | - | 50.7 ± 7.1b | - | - | ||
control | - | 25.3 ± 9.3ab | - | - | ||
Baiting** | no food | Mortierella signyensis | - | 6.7 ± 1.3a | - | - |
Mortierella alpina | - | 9.3 ± 5.3a | - | - | ||
control | - | 8.0 ± 4.6a | - | - |
- = trial not done for this species; *mortality of Musca domestica was a function of non-emergence from puparia; **insects exposed to soil + fungus, with absence of food source (final fungal load may differ between dishes).
Inoculation was via dipping, injection, soil treatment and baiting. All trials were run at 10°C. Mortality was assessed after 5 d for injected insects and 4 wk for soil inoculation. For each insect and for each inoculation method, figures with the same letter within each column are not significantly different (P > 0.05).