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. 2014 Jun 6;127(7):1561–1581. doi: 10.1007/s00122-014-2326-7

A consensus map for Ug99 stem rust resistance loci in wheat

Long-Xi Yu 1,5, Hugues Barbier 1, Matthew N Rouse 4, Sukhwinder Singh 2, Ravi P Singh 2, Sridhar Bhavani 2, Julio Huerta-Espino 3, Mark E Sorrells 1,✉
PMCID: PMC4072096  PMID: 24903979

Abstract

Key message

This consensus map of stem rust genes, QTLs, and molecular markers will facilitate the identification of new resistance genes and provide a resource of in formation for development of new markers for breeding wheat varieties resistant to Ug99.

Abstract

The global effort to identify new sources of resistance to wheat stem rust, caused by Puccinia graminis f. sp. tritici race group Ug99 has resulted in numerous studies reporting both qualitative genes and quantitative trait loci. The purpose of our study was to assemble all available information on loci associated with stem rust resistance from 21 recent studies on Triticum aestivum L. (bread wheat) and Triticum turgidum subsp. durum desf. (durum wheat). The software LPmerge was used to construct a stem rust resistance loci consensus wheat map with 1,433 markers incorporating Single Nucleotide Polymorphism, Diversity Arrays Technology, Genotyping-by-Sequencing as well as Simple Sequence Repeat marker information. Most of the markers associated with stem rust resistance have been identified in more than one population. Several loci identified in these populations map to the same regions with known Sr genes including Sr2, SrND643, Sr25 and Sr57 (Lr34/Yr18/Pm38), while other significant markers were located in chromosome regions where no Sr genes have been previously reported. This consensus map provides a comprehensive source of information on 141 stem rust resistance loci conferring resistance to stem rust Ug99 as well as linked markers for use in marker-assisted selection.

Electronic supplementary material

The online version of this article (doi:10.1007/s00122-014-2326-7) contains supplementary material, which is available to authorized users.

Introduction

Wheat stem rust caused by the pathogen Puccinia graminis Pers. f. sp. tritici Eriks. and E. Henn., is one of the most destructive wheat diseases. It can cause up to 90 % yield loss in wheat production but has been effectively under control due to the successful deployment of resistance genes in wheat cultivars since the 1950s (McIntosh et al. 1995). However, the outbreak of a new stem rust race in Uganda named Ug99 (race TTKSK; Pretorius et al. 2000) spread throughout much of Africa, the Middle East and Iran and poses an imminent threat to wheat production worldwide (Singh et al. 2006; Sharma et al. 2013).

To improve the efficiency of wheat breeding for durable resistance to stem rust, it is essential to understand the genetic basis. To-date, 58 stem rust resistance (Sr) genes have been numerically designated in wheat as part of the International Wheat Genetics Symposium Gene Catalog (McIntosh et al. 1995, 2011). Several alleles conferring unique race specificities have been identified for many of these genes resulting in a total of 65 numerically designated resistance genes and alleles. Of these genes and alleles, phenotypic data have been published indicating that at least 27 are effective or partially effective to the Ug99 race group: Sr2 (Yr30), Sr13, Sr21, Sr22, Sr24, Sr25, Sr26, Sr27, Sr28, Sr32, Sr33, Sr35, Sr36, Sr37, Sr39, Sr40, Sr42, Sr44, Sr45, Sr46, Sr47, Sr51, Sr52, Sr53, Sr55 (Lr67/Yr46/Pm46), Sr57 (Lr34/Yr18/Pm38), Sr58 (Lr46/Yr29/Pm39) (Faris et al. 2008; Ghazvini et al. 2012; Kolmer et al. 2011; Jin and Singh 2006; Jin et al. 2007; Liu et al. 2011a, b; McIntosh et al. 2012; Rouse et al. 2011; Rouse and Jin 2011; Singh et al. 2013b, c). Several additional resistance genes have been characterized as resistant to Ug99; however, their relationship to numerically designated genes has not been determined: SrAes7t, SrCad, SrND643, SrTA10171, SrTA10187, SrTA1662, SrTmp, SrWeb, Sr1RS Amigo (Hiebert et al. 2010, 2011; Jin and Singh 2006; Jin et al. 2007; Klindworth et al. 2012; Olson et al. 2013). It is possible that such genes with temporary designations are redundant with, or are alleles of, numerically designated genes. Several of the numerically designated Sr genes are qualitative and race specific (Jin et al. 2007; Singh et al. 2011). Qualitative genes are phenotyped as present or absent, often at the seedling stage by observing the characteristic low or high infection types displayed by them as described by Jin et al. (2007). One strategy for achieving durable resistance is to pyramid multiple qualitative resistance genes into each wheat variety. A major risk associated with the utilization of qualitative resistance genes is the ability of pathogens to defeat such genes when they are deployed alone in wheat cultivars as has been demonstrated by the original Ug99 defeating Sr24, Sr36, and resistance in cultivar ‘Matlabas’ resulting in “Boom and Bust” cycles (Jin et al. 2008, 2009; Pretorius et al. 2012).

Utilization of quantitative resistance, often based on multiple minor genes that slow down pathogen infection and colonization in adult plants, referred to as ‘Adult Plant Resistance’ (APR) (Gustafson and Shaner 1982), is another approach for achieving durable resistance. Adult plant resistance to stem rust in wheat is a complex trait conferred by quantitative trait loci (QTL). This type of resistance can be more durable than single gene resistance due to race non-specificity of the resistance genes involved. A total of five numerically designated wheat stem rust resistance genes confer quantitative APR: Sr2, Sr55 (Lr67/Yr46/Pm46), Sr56, Sr57 (Lr34/Yr18/Pm38), and Sr58 (Lr46/Yr29/Pm39). In addition, several recent studies have identified numerous QTL associated with wheat stem rust resistance in diverse germplasm (Bansal et al. 2008; Bhavani et al. 2011; Crossa et al. 2007; Kaur et al. 2009; Njau et al. 2013; Rouse et al. 2014; Singh et al. 2013a, b, c; Yu et al. 2011, 2012).

The global effort to identify new sources of resistance to Ug99 has led to the identification of putative new qualitative and quantitative resistance loci reported in numerous studies in the past few years in different populations. As resistance loci are reported, it is important to determine their potential redundancy in order to prioritize those that can be deployed in a breeding program. The purpose of this study was to compile available information on Ug99 resistance loci and their map locations in a single consensus map to facilitate future mapping studies. The map locations from various sources were analyzed along with our recent association mapping projects involving 608 spring and winter wheat breeding lines from CIMMYT and the International Winter Wheat Improvement Program (Yu et al. 2011, 2012). The consensus map contains Diversity Arrays Technology (DArT), Single Nucleotide Polymorphism (SNP), Genotyping-by-Sequencing (GBS) and Simple Sequence Repeat (SSR) markers to facilitate cross-referencing markers and Ug99 resistance loci with other maps.

Materials and methods

Literature review and synthesis of stem rust resistance loci

Stem rust resistance loci data were collected from 21 recent studies, for a total of 24 biparental mapping populations, 3 association panels, 2 BC2 populations, and two from resistance gene cloning (Bansal et al. 2008; Bhavani et al. 2011; Crossa et al. 2007; Ghazvini et al. 2012; Haile et al. 2012; Hiebert et al. 2010, 2011; Kaur et al. 2009; Letta et al. 2013; Olson et al. 2013; Njau et al. 2013; Periyannan et al. 2013; Rouse et al. 2012, 2014; Saintenac et al. 2013; Singh et al. 2013a, b, c; CIMMYT unpublished, Yu et al. 2011, 2012) (Table 1). The details of the reports, including the chromosome positions of stem rust resistance loci, their LOD (log (Odds)) scores and p values, parents used to develop the population as well as stem rust pathotypes for disease reaction are presented in Table 1. Three of the studies did not determine stem rust resistance loci by screening with Ug99 (Bansal et al. 2008; Crossa et al. 2007; Kaur et al. 2009). Though included these loci in our study, their effectiveness against Ug99 is unknown and are presented because of their relevance to Ug99 studies.

Table 1.

Stem rust resistance loci identified in 21 studies including 24 mapping populations, three association panels, and two BC2 populations

Sr locus name Phenotypic variation (R^2) Chromosome Start End Resistance carrier Population References
Marker Position cM Marker Position cM
Biparental population QSr.Sun-5BL 12 % 5BL* ksuHb9 34.2 glk0354 44.8 Arina Arina/Forno Bansal et al. (2008)
QSr.Sun-7DS 26 % 7DS* cdo0475b 90.2 gwm1002 94.8 Arina
Across all data set QSr.Sun-3BS 9–15 % 3BS wPt-8093 31.4 gwm566 61 HD2009 HD2009/WL711 Kaur et al. (2009)
QSr.Sun-5DL 20–44 % 5DL gwm182 60.2 SrHD 85.3 HD2009
QSr.Sun-7A 7–13 % 7A* wPt-3992 136.1 gwm260 157.3 HD2009
Envir. specific QSr.Sun-2B 8–13 % 2B wPt-4892 99 wPt-4892 104.4 HD2009
QSr.Sun-5B 11–15 % 5BS* wPt-6136 6 wPt-5346 34.1 HD2009
QSr.Sun-4B 9 % 4B wPt-5559 6 wPt-8756 8.4 WL711
QSr.Sun-lD 12 % ID* wPt-4687 4.9 – – HD2009
Sr30 – 5DL* CFD12 9 gwm292 16 Webster RL6071/Webster Hiebert et al. (2010)
SrWeb – 2BL gwm47 1.4 wmc332 12.4 Webster
– – 1A wPt-0128 – wPt-734078 – Kingbird PBW343/Kingbird Bhavani et al. (2011)
– – 3BS wPt-3921 – wPt-2757 – Kingbird
– 51.2 5BL wPt-2607 – wPt-1733 – Kingbird
– – 7A wPt-8670 – wPt-744574 – Kingbird
– – 7DS wPt-1859 – wPt-731810 – Kingbird
– 6 2D* barc095 – – – Kiritaki PBW343/Kiritaki
– 25 3BS* SW58 – – Kiritaki
– 8 5BL* barc109 – – Kiritaki
– 12 7D Lr34-linked – – – Kiritaki
– – 2B* wPt-7829 – wPt-2266 – Juchi PBW343/Juchi
– – 3BS wPt-8056 – wPt-800213 – Juchi
– 42.4 4A wPt-5124 – wPt-6390 – Juchi
– – 5BL wPt-0750 – wPt-5896 – Juchi
– – 6B wPt-5480 – wPt-9532 – Juchi
– 6.8 2B – – wmc257 – Huirivis#1 PBW343/Huirivis#1
– 16 3BS* – – SW3648 – Huirivis#1
– 23 5BL* wms371 – NW2012ND – Huirivis#1
– 6.9 7B* – – NW3109ND – Huirivis#1
– – 2B wPt-744022 – wPt-1964 – Muu PBW343/Muu
– 46 3BS wPt-666139 – wPt-3921 – Muu
– – 5BL wPt-6014 – wPt-3661 – Muu
– – 1BL wPt-1560 – wPt-7486 – Pavon76 Avocet/Pavon76
– 68.9 3BS wPt-8093 – wPt-7212 – Pavon76
– – 5A wPt-6048 – wPt-4249 – Pavon76
– – 6B wPt-1541 – wPt-0171 – Pavon76
Sr28 – 2BL wcm332 0 wPt-7004 7.6 SD1691 SD1691/LMPG-6 Rouse et al. (2012)
Sr42 – 6BS* barc183 5.5 FSD_RSA 6 Norin 40 Norin 40/LMPG-6 Ghazvini et al. (2012)
– 15.1 1BL* wPt-8894 – wPt-6015 – – PBW343/Kenya Nyangumi
– 22.4 2BL wPt-2274 118.2 wPt-2135 118.2 –
– 8.8 3BS wPt-800213 23.3 wPt-742648 26.7 –
– 33.7 5BS* wPt-8604 25.6 wPt-7542 – –
– 56.9 6BS wPt-4283 5 wPt-7207 7 –
– 6.6 7AS wPt-742244 2.9 wPt-672171 5.6 –
– 10 7BL wPt-0138 120.6 wPt-4258 – –
– 24.3 1B* wPt-0320 – wPt-1911 – – PBW343/Cross Bill
– 14.8 3BL* wPt-8105 – wPt-10003 – –
– 23.5 3BL wPt-6047 – wPt-9433 – –
– 55.3 3BS* wPt-3609L3R3 – wPt-3609L2R2 – –
– 8.6 6BS wPt-5461 – barc79 – –
– 19.4 3BS wPt-6043 14.2 wPt-742337 15.6 – PBW343/Diniza
– 50.3 3BS* wPt-2220 67.3 wPt-4364 68.6 – CIMMYT unpublished
– 7.8 7AL wPt-741686 150.9 wPt-7763 168.5 –
– 20.9 1AL* wPt-732616 133.1 wPt-4065 113.4 – PBW343/Kenya Swara
– 22.4 3BS* wPT-800213 17.1 wPt-3921 26.7 –
– 31.2 6AS wPt-669498 0 wPt-6520 2.5 –
– 5.4 7AL wPt-6495 150.9 wPt-741686 154.8 –
– 4.2 7DS* wPt-743395 – wPt-742859 – – PBW343/Kenya Kudu
– 12 1AS wPt-0512 4.6 wPt-1770 – –
– 17 2BL wcm474 54.4 wPt-2600 70.4 –
– 15 3AL wPt-9154 73.4 cfd2 – –
– 30 3BS gwm533 – wPt-3921 17.1 –
– 12 5BL wPt-0750 102.7 wPt-3114 117.6 –
– 10 7BL wPt-8007 140.8 wPt-5460 145.2 –
– 23.8 1BL wPt-1560 8.6 wPt-7486 – Pavon76 Avocet/Pavon76 Njau et al. (2013)
– 6 IDS wPt-7140 57.1 wPt-5320 60.7 Pavon76
– 7 2AL wPt-7024 71 wPt-7011 76 Pavon76
– 5.5 2BL* wPt-4125 82.7 wPt-1505 102.4 Pavon76
– 51.8 3BS wPt-1081 17.1 wPt-3781 24.9 Pavon76
– 18.1 7BL wPt-4319 143 wPt-3190 – Pavon76
QSr.cdl-1AL – 1AL wPt-0827 – wPt6869 – Thatcher Thatcher/McNeal Rouse et al. (2014)
QSr.cdl-3BS (Sr12) – 3BS gpw1120 – wPt4082 – Thatcher
QSr.cdl -2BS – 2BS wPt-2106 – cfd238 – Thatcher
Sr57 (Lr34) – 7DS* csffr6 – – – McNeal
QSr.ipk-1A 3.7 1AL Xbarcl48 121.6 Xbarc119 137.7 Sebatel Kristal/Sebatel (durum) Haile et al. (2012)
QSr.ipk-2A 3.9 2AS* Xgwm448 66 Xgwm1198 79.5 Sebatel
QSr.ipk-3B 8.6 3BS* Xgwm779 0 Xgwm389 9.6 Sebatel
QSr.ipk-4B 3.8 4BL* Xgwm1167 24.6 Xgwm1278 83.1 Sebatel
QSr.ipk-5B 3.7 5BL Xgwm408 56 Xbarc142 83.1 Sebatel
QSr.ipk-6A 6.5 6AL* Xgwm494 104.7 Xgwm11150 124.7 Sebatel
QSr.ipk-7A.1 3.9 7A* Xgwm974 106.3 Xgwm631 120.8 Sebatel
QSr.ipk-7A.2 3.4 7AL Xbarc121 144.2 Xgwm984 154.4 Sebatel
QSr.ipk-7B 7.3 7 BL* Xgwm146 127.7 Xgwm344 152.6 Sebatel
– 14.5 4B* wPt-0872 – – – – Sachem/Strongfield (durum) Singh et al. (2013a)
QSr.spa-4B.1 2.4-6.1 % 4B* wPt-744434 62.3 Xwmc-617 89.4 Carberry AC Cadillac/Carberry Singh et al. (2013b)
QSr.spa-6D 3.9-48.8 % 6D* wPt-664770 0 wPt-1695 9.7 AC Cadillac
Association mapping 12 1BS wPt-1560 8.6 wPt-5678 33.7 – LD-SRRSN (Spring) Yu et al. (2011)
7 2BL wPt-7200 82 wPt-8460 101 –
8 3BS wPt-6945 57 wPt-1940 68 –
7 3BS csSr2 0 wPt-8446 11 –
6 4AL wPt-5857 68 wPt-5749 72 –
5 5BS wPt-1149 31 wPt-5346 33 –
5 6AL wmc417 88 gwm617 97 –
5 6AS wPt-6520 2 wPt-4016 4 –
14 6BS wPt-3774 0 wPt-1922 4 –
11 6BS wPt-5333 31 wPt-5037 57 –
12 7BL wPt-5343 126.1 wPt-7351 126.8 –
14 7DL wPt-1859 116 wPt-7351 126.8 –
15 7DL wPt-7763 160 wPt-664017 175 –
8 7DS wPt-2565 1.4 wPt-665260 5 –
6 1AS wPt-730213 0.2 wPt-730213 0.2 – LD-SRRSN (Winter)
7 2BS wPt-4916 2 wPt-1964 25 –
8 3BS csSr2 0 wPt-8446 11.4 –
5 4AL wPt-3349 84.5 wPt-7807 87.8 –
5 5BS wPt-1302 1 wPt-3873 27 –
5 6BL wPt-6116 107 wPt-6116 107 –
6 6BS wPt-4930 9 wPt-1241 24 –
5 6BS wPt-4648 51 wPt-4648 51 –
6 7BS wPt-0138 18.9 wPt-0138 18.9 –
10 7DS csLV34 50.5 csLV34 50.5 –
Sr31 – 1BS wPt-8949 11.4 wPt-8616 32.3 – ESWYT Crossa et al. (2007)
Sr19,23,36,40 – 2B wPt-0100 17.7 – – –
Sr19,23,36,40 – 2B wPt-9402 57.8 – – –
Sr2,12 – 3B* wPt-0365 44 wPt-8983 44 –
Sr2,12 – 3B* wPt-6802 56.9 – – –
– – 4AS wPt-2788 58 – – –
– – 4AL wPt-2084 161 wPt-7807 161.7 –
– – 4BS wPt-8650 7.8 – – –
– – 5BL wPt-4996 82.8 – – –
Sr13, 26 – 6AL wPt-7623 38 – – –
– – 6BS wPt-7662 36.8 wPt-7777 41.9 –
– – 6BS wPt-3733 87.5 – – –
Sr11 – 6BL wPt-0171 171.4 wPt-1541 181 –
Sr17 – 7B wPt-1149 64.7 – – –
Sr17 – 7B wPt-5343 99 – – –
Sr17 – 7B wPt-0600 114.3 – – –
Sr44 – 7D wPt-2565 1.3 – – –
– 4.6 1BS* barc8 32 – – – AM durum panel Letta et al. (2013)
– 3.9 2AS* gwm1045 87.7 – – –
– 4.1 2BL* wmc356 220 – – –
– 3.3 3AS wPt-7992 8 – – –
– 4 3AL* wmc388 85.6 – – –
– 4.1 5AL* gwm126 93.3 – – –
– 4.4 5AL gwm291 111.7 – – –
– 3.5 6AL gwm427 139.5 – – –
– 7.1 6AL* CD926040 144 – – –
– 4.5 6AL* barc104 155.3 – – –
– 5.2 7AS* wPt-2799 38.2 – – –
– 1.5 7AS* wPt-7785 94.8 – – –
Direct back crossing SrTA10187 – 6DS* Xcfd49 5.4 Xbarc173 20.9 Aa. tauschii- TA10187 BC2-TA10187/KS05HW14 Olson et al. (2013)
SrTA10171 – 7DS* – – Xwmc827 0.9 Aa. tauschii- TA10171 BC2-TA10171/KS05HW14
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* indicates QTL that are not displayed on the map

Construction of a consensus map

A consensus genetic map was constructed using the Wheat Interpolated Maps v4 (Diversity Arrays Technology Pty. Ltd., personal communication) as a reference map.

(http://www.triticarte.com.au/). DArT, SNP, GBS and SSR markers from the Wheat KASPar SNP database (http://www.cerealsdb.uk.net/CerealsDB/SNPS/), the 2004 Wheat SSR Consensus Map (Somers et al. 2004), and the Thatcher/McNeal map (Sherman et al. 2013) with concatenated DArT and SSR markers were integrated to the reference map using the ad hoc R package “LPmerge” (Endelman and Plomion 2011) and BioMercator V3.0 (http://moulon.Inra.fr). LPmerge is an optimized “synthetic” (Wenzl et al. 2006) or “composite” (Hudson et al. 2012) approach to built a map across multiple populations. As opposed to minimizing an objective function based on the observed recombination frequencies between markers (JoinMap; Van Ooijen 2006) or (MultiPoint; Ronin et al. 2012), this R package based its algorithm directly on the component linkage maps instead of the recombination frequencies. Other software uses the same approach, but LPmerge implements an additional algorithm for resolving ordinal conflicts found when the marker order was not consistent between the different linkage maps. Resistance loci or QTL associated with stem rust resistance identified from 21 studies and one personal communication (Table 1) were projected onto the consensus map based on the position of the markers linked to the loci when the precision of the constructed map allowed it (Fig. 2). A star following the chromosome number on Table 1 tags the QTL not present on Fig. 2.

Fig. 2.

Fig. 2

Fig. 2

Fig. 2

Fig. 2

Fig. 2

Fig. 2

Fig. 2

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A consensus map of stem rust resistance loci in wheat. The map was constructed using the Wheat Interpolated Maps v4 (Diversity Arrays Technology Pty. Ltd., personal communication) as a reference map. DArT (prefix “wPt”), SNP (prefix “BS” or “SNP”) and SSR markers linked to stem rust resistance loci were integrated as described in the “Materials and methods”. The bars with different colors or patterns on the left side of chromosome regions distinguish mapping populations used in each study as shown in Table 1. Major genes that overlapped with QTLs were added to the right side of chromosomes based on the positions of the linked markers. A.M. association mapping, B.P. bi-parental population

Results

Overview of loci for stem rust resistance

The constructed consensus map contains a total of 1,433 markers distributed among the 21 chromosomes. Fourteen percent of these markers are SNP, 16.7 % are GBS, 39.4 % are DArT and 29.9 % are SSR. With a global average distance of 2.7 cM between markers, the maximum average distance between markers is observed on chromosome 5D (16 markers) with 7.6 cM and the minimum average distance between markers is observed on chromosome 3B (158 markers) with 1.9 cM. An expected negative correlation is observed between the marker number per chromosome and average distance in centimorgan between markers (r = 0.719). The average chromosome size was 150 cM with a maximum of 203.3 cM for chromosome 7A and a minimum of 83.4 cM. Resistance gene and QTL maps and gene cloning information from 21 studies and one personal communication (Table 1) involving 24 mapping populations, three association panels, and two BC2 populations were concatenated to construct a map for stem rust resistance loci (Fig. 1). The map consists of 141 stem rust resistance loci distributed across the genome, many of which were redundant loci detected in at least two studies. A total of 37, 86 and 18 resistance loci were located in the A, B, and D genomes, respectively (Fig. 1). Several hotspots of resistance loci were observed across the genome. Nineteen were located on chromosome arm 3BS, while 6BS, 5BL and 2BL had nine, nine, and seven QTL, respectively. Among these hotspots, qualitative genes for stem rust resistance have been mapped on 3BS, 2BL and 5BL, while no qualitative genes have been characterized on 6BS. Clusters of QTLs were located distally on 5BS, 6BS and 7AS in regions where no previously reported qualitative or quantitative genes are located.

Fig. 1.

Fig. 1

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a Distribution of QTL associated with stem rust resistance by chromosome arm based on 21 studies. b Distribution of QTL associated with stem rust resistance by chromosome based on 21 studies. c Distribution of QTL associated with stem rust resistance by genome based on 21 studies

Chromosome group 1 had 15 QTL and the cloned gene, Sr33, across the three homoeologous chromosomes. Six QTL were found on 1A (two on the short arm, three on the long arm and one is still in an undetermined arm location), five using bi-parental crosses (Thatcher/McNeal: Rouse et al. 2014; PBW343/Kingbird: Bhavani et al. 2011; PBW343/Kenya Swara; Kristal/Sebatel: Haile et al. 2012 and PBW343/Kenya Kudu: CIMMYT unpublished) and one using association mapping (LD-SRRSN (winter): Yu et al. 2012). Five of these QTL are represented in Fig. 2. One qualitative Ug99 resistance gene was mapped on 1A: Sr1RS. Amigo (Schlegel and Kynast 1987; Jin and Singh 2006; McIntosh et al. 2012). Seven QTL were found on 1B (three on the short arm, three on the long arm and one is still in an undetermined arm location). Four were mapped using bi-parental crosses (PBW343/Crossbill, PBW343/Kenya Nyangumi: CIMMYT unpublished; Avocet/Pavon76: Njau et al. 2013; Bhavani et al. 2011) and three using association mapping (ESWYT: Crossa et al. 2007; LD-SRRSN (spring): Yu et al. 2011 and the AM durum panel: Letta et al. 2013). Four of these QTL are represented in Fig. 2. Sr31 (marker XwPt-8949) is on a 1BL.1RS rye translocation and is homologous with at least three of the QTL found in that region. Sr14, from tetraploid wheat origins (Heerman and Stoa 1956), was also reported on 1B, close to the centromere (McIntosh et al. 1995). The response of Sr14 to Ug99 has been reported as inconclusive by Jin et al. (2007) and is not displayed in Fig. 2. Two QTL were mapped on chromosome 1D (one on the short arm and one in an undetermined arm location) using bi-parental crosses (HD2009/WL711: Kaur et al. 2009; Avocet/Pavon76: Njau et al. 2013). One of these QTL is represented in Fig. 2. The QTL linked to XwPt-7140 on 1DS coincides with at least one of the four known Sr genes located in that region (SrTA1662, Sr33, Sr45, Sr50: Rouse et al. 2011; Sambasivam et al. 2008; Anugrahwati et al. 2008).

Chromosome group 2 had 19 QTL across the three homoeologous chromosomes. The short arm of chromosome 2A has two QTL where the centromeric stem rust resistance gene Sr21 (The 1973) is located and the long arm had one QTL. One of these is represented in Fig. 2. Two Sr genes have been described on chromosome 2A, Sr21 (The 1973), derived from Triticum monococcum L., as well as Sr32 (McIntosh et al. 1995). It has to be noted that stem rust gene Sr32 has also been mapped on chromosome 2B (McIntosh et al. 2011) as well as 2D (Mago et al. 2013). These three locations are displayed in Fig. 2, and Sr32 is displayed distally to each chromosome 2 arm in Fig. 2. Chromosome 2B is the location of 15 QTL (two on the short arm, seven on the long arm and the arm location of six loci are unknown), 10 mapped in bi-parental crosses (Avocet/Pavon76: Njau et al. 2013; PBW343/Kenya Nyagumi and PBW343/Kenya Kudu: CIMMYT unpublished; PBW343/Juchi, PBW343/Huirivis#1, PBW343/Muu: Bhavani et al. 2011; HD2009/WL711: Kaur et al. 2009; RL6071/Webster: Hiebert et al. 2010; SD1691/LMPG-6: Rouse et al. 2012; Thatcher/McNeal: Rouse et al. 2014) and 5 detected using association mapping (ESWYT: Crossa et al. 2007; LD-SRRSN (spring); AM durum panel: Letta et al. 2013 and LD-SRRSN (winter): Yu et al. 2011). Twelve of these loci are shown in Fig. 2. There are 11 designated Sr genes on 2B (seven of them are shown in Fig. 2) in addition to Ug99-effective SrWeb (Hiebert et al. 2010). At least two QTL were identified on 2BS where seven Sr genes have been reported. Among them, Sr39 (Niu et al. 2011), Sr40 (Wu et al. 2008), and Sr36 (Rouse et al. 2012) are effective against the original Ug99 race TTKSK and became ineffective to the variant TTTSK (Jin et al. 2009). Resistance genes SrWeb, Sr28, and Sr47 have been reported on chromosome arm 2BL (Faris et al. 2008; Hiebert et al. 2011; Rouse et al. 2012). Chromosome 2D also had a single QTL in a bi-parental cross (PBW343/Kiritati: Bhavani et al. 2011; location not shown). Chromosome 2DS carries two qualitative Sr loci (Sr32 and Sr46; Mago et al. 2013; E. Lagudah, personal communication, 2010) at the position of the QTL near marker Xbarc90.

On chromosome group 3, 26 QTL were reported as well as a cloned gene across the three homoeologous chromosomes. A single QTL was mapped on 3AS using association mapping (AM durum panel: Letta et al. 2013) and two QTL were mapped on 3AL, one using a bi-parental cross (PBW343/Kenya Kudu: CIMMYT unpublished) and one by association mapping (AM durum panel: Letta et al. 2013). Two of these QTL are shown in Fig. 2. Chromosome 3A carries two Sr genes, one cloned by Saintenac et al. (2013), Sr35 on the long arm and the other one, Sr27, is located on the short arm (Acosta 1963). The other 23 QTL were on 3B (2 are on the long arm, 19 on the short arm and the locations of 2 are unknown). Eighteen were mapped using bi-parental crosses (Thatcher/McNeal: Rouse et al. 2014; PBW343/Crossbill, PBW343/Kenya Nyangumi, PBW343/Diniza, PBW343/Kenya Swara, PBW343/Kenya Kudu and PBW343/Pavon76: CIMMYT unpublished; HD2009/WL711: Kaur et al. 2009; PBW343/Kingbird, PBW343/Kiritaki, PBW343/Juchi, PBW343/Huirivis#1, PBW343/Muu and Avocet/Pavon76: Bhavani et al. 2011 and Kristal/Sebatel: Haile et al. 2012). Five were mapped by association analysis (ESWYT: Crossa et al. 2011; LD-SSRN (spring): Yu et al. 2011). Ten of the mapped loci are shown in Fig. 2. Eleven of the QTL on chromosome 3BS are coincident with the slow rusting gene, Sr2, that contributes to APR (Singh et al. 2006). QTL near the centromere of chromosome 3B appear to be linked to the seedling resistance gene Sr12. Though Sr12 was not characterized as effective as Ug99 (Jin et al. 2007), recent data suggest that it interacts with other resistance loci to confer APR to Ug99 (Rouse et al. 2014).

Ten QTL were located on group 4 chromosomes. One QTL was located on 4AS, three were on 4AL and one was at an unknown location. Among them, one was identified using a bi-parental cross (PBW343/Juchi: Bhavani et al. 2011) and four using association mapping (LD-SRRSN (winter) and LD-SRRSN (spring): Yu et al. 2011; ESWYT: Crossa et al. 2007). Chromosome 4A carries one Sr gene temporarily designated as SrND643 (CIMMYT unpublished), overlapping with QTL from several studies. Though Ug99 effective resistance gene Sr37 has been introgressed into chromosome 4B, this translocation has not been used in a breeding program (McIntosh et al. 1995). We did not identify any QTL coincident with the previously described APR gene Sr55 (Lr67/Yr46/Pm46) (McIntosh et al. 2012) on chromosome arm 4DL.

Group 5 had 18 QTL, 3 of which were on chromosome 5A. One of them was identified using a bi-parental cross (Avocet/Pavon76: Njau et al. 2013), and two by association mapping (AM durum panel: Letta et al. 2013). Two of these loci are shown in Fig. 2. Thirteen QTL were found on chromosome 5B, ten using bi-parental crosses (Arina/Forno: Bansal et al. 2008; PBW343/Kingbird, PBW343/Kiritaki, PBW343/Juchi, PBW343/Huirivis#1 and PBW343/Muu: Bhavani et al. 2011; PBW343/Kenya Kudu and PBW343/Kenya Nyangumi: CIMMYT unpublished; Kristal/Sebatel: Haile et al. 2012) and three using association mapping (ESWT: Crossa et al. 2007; LD-SSRN (Spring) and LD-SSRN (winter): Yu et al. 2011). Eight of the 5B QTL are shown in Fig. 2. Kaur et al. (2009) detected a distal 5BS QTL in three of the four environments tested using a bi-parental population. This finding was confirmed by Yu et al. (2011) in the detection of two QTL distally located on chromosome 5BS by association mapping in spring and winter CIMMYT germplasm between markers XwPt1149/XwPt 5346 and XwPt1302/XwPt3873, respectively. Both QTL were minor but overlapped with the QTL detected by Kaur et al. (2009) (marker wPt5346). The QTL on 5BL from the Arina/Forno population was recently designated as Sr56 (McIntosh et al. 2012) and confers APR. No other stem rust resistance genes have been designated on chromosome 5B. Ug99 resistance gene Sr53 was recently introgressed from Aegilops geniculata to chromosome arm 5DL where two Ug99 QTL were identified using bi-parental crosses (HD2009/WL711: Kaur et al. 2009; RL6071/Webster: Hiebert et al. 2010).

Group 6 had 23 QTL across the three homoeologous chromosomes. On chromosome 6A two QTL were on the short arm and six on the long arm. Two were mapped using a bi-parental cross (PBW343/Kenya Swara: CIMMYT unpublished; Kristal/Sebatel: Haile et al. 2012) and six using association mapping (ESWT: Crossa et al. 2007; LD-SRRSN (spring): Yu et al. 2011; AM durum panel: Letta et al. 2013). Five of the 6A loci are represented in Fig. 2. Three designated Sr genes are located on 6A, including three Ug99 resistance genes located on chromosome arm 6AL (Sr26, Sr13, and Sr52; Hart et al. 1993; McIntosh 1972; Qi et al. 2011). Sr52 is not currently used in breeding programs. On 6B there were 13 QTL (nine on the short arm, two on the long arm and two have an unknown arm location), 5 were mapped in bi-parental crosses (PBW343/Juchi, Avocet/Pavon76: Bhavani et al. 2011; PBW343/Crossbill, PBW343/Kenya Nyangumi: CIMMYT unpublished; Norin40/LMPG-6: Ghazvini et al. 2012) and 8 by association analysis (LD-SRRSN (spring) and LD-SRRSN (winter): Yu et al. 2011; ESWYT: Crossa et al. 2007). Twelve of these QTL are represented in Fig. 2. No major Ug99 resistance genes are known to be located in this region. The 6DS chromosome arm had one resistance gene identified in a BC2 population (SrTA1662; BC2-TA10187/KS05HW14: Olson et al. 2013) and a QTL identified in bi-parental crosses (AC Cadillac/Carberry: Lopez-Vera et al. 2014). Neither of the 6DS loci are shown in Fig. 2 because of difficulty in cross referencing the chromosome location. Four Ug99 resistance Sr genes have been identified on chromosome 6D. Three of them are located on the short arm, Sr42, SrTA10187 and SrCad (Hiebert et al. 2011; Ghazvini et al. 2012; Lopez-Vera et al. 2014) and one on the long arm, Sr29 (Dyck and Kerber 1977).

Group 7 had 30 QTL across the three homoeologous chromosomes. Three were mapped on 7AS, three on 7AL, and three have unknown locations. Seven were mapped in bi-parental populations (HD2009/WL711: Kaur et al. 2009; PBW343/Kingbird: Bhavani et al. 2011; PBW343/Diniza, PBW343/Kenya Swara, PBW343/Kenya Nyangumi: CIMMYT, unpublished; Kristal/Sebatel: Haile et al. 2012) and two were mapped by association mapping (AM durum panel: Letta et al. 2013). Five of these QTL are shown in Fig. 2. Gene Sr15 (not shown in Fig. 2) and Ug99 effective gene Sr22 (The 1973) are located on 7AL. On 7B there were ten QTL, five of which were mapped using bi-parental crosses (PBW343/Huirivis#1: Bhavani et al. 2011; PBW343/Kenya Nyangumi, PBW343/Kenya Kudu: CIMMYT, unpublished; Avocet/Pavon76: Njau et al. 2013; Kristal/Sebatel: Haile et al. 2012) and five using association analysis (ESWYT: Crossa et al. 2007; LD-SRRSN (spring) and LD-SRRSN (winter): Yu et al. 2011). Seven of these QTL are represented in Fig. 2. Eight of these QTL are mapped near Sr17 (position not represented in Fig. 2) (Bansal et al. 2008). The 7D chromosome had 11 QTL, 6 mapped in bi-parental crosses (Thatcher/McNeal: Rouse et al. 2014; PBW343/Kiritaki, PBW343/Kingbird: Bhavani et al. 2011; Arina/Forno: Bansal et al. 2008; PBW343/Kenya Swara: CIMMYT, unpublished; BC2-TA10171/KS05HW14: Olson et al. 2013), whereas five were mapped in association panels (ESWYT: Crossa et al. 2007; LD-SRRSN (spring) and LDSRRSN (winter): Yu et al. 2011). Seven of these QTL are shown in Fig. 2. Pleiotropic rust and powdery mildew resistance gene Sr57 (Lr34/Yr18/Pm38), Sr44 as well as SrTA10171 are located on 7DS (Kolmer et al. 2011; Bernd Friebe, personal communication) and Sr25 as well as Sr43 (Xu et al. personal communication) are located on 7DL.

Discussion

Four maps including the Wheat Interpolated DArT Maps v4, the wheat consensus SSR map, the wheat KASPar SNP map, and the Thatcher/McNeal DArT/SSR markers map were used to build the consensus map for locating the stem rust resistance loci (Fig. 2). The consensus map consisted of 1,433 markers and an average marker interval of 11.5 cM. We focused on integrating markers in the regions spanning stem rust resistance QTL that can further facilitate fine mapping and cross referencing the locations with other maps. Therefore, the marker density in the QTL regions is higher than the average. For example, marker density is almost three times higher in the QTL region of rust resistance genes on 1B (Fig. 2, Chromosome 1B). The same is true for 2BL, 3BS, 4AL, 6BS and 7DL. However, the complexity and context dependency of QTL identified in different genetic backgrounds and environments can limit the accuracy of the locations. The accurate genome location of QTL and major genes across genetic backgrounds and environments is a prerequisite for the use of the QTL in MAS. Meta-analysis of QTL identified in different studies can locate QTL more precisely, thus facilitating the identification of closely linked markers for MAS. Because many of the populations from which APR was assessed lacked Ug99-effective qualitative resistance genes, the coincidence of APR with seedling resistance was not likely to be a result of the qualitative resistance genes conferring APR. For example, on 2BS, seven QTL were identified that overlapped with two Ug99-effective stem rust resistance genes, Sr39 and Sr36 (Fig. 2b). Since Sr39 was not present in the wheat cultivars used to map the QTL and Sr36 was only present in the germplasm analyzed by Yu et al. (2012) where no association was found between Sr36 and APR, the QTL on 2BS could be conferred by alleles of these qualitative genes, residual effects of other Sr genes on 2BS, or new genes. Similarly, the stem rust resistance gene Sr40 on 2BS is strongly associated with marker Xgwm388 and coincided with one QTL, however Sr40 was not present in the corresponding germplasm (Yu et al. 2011). Allelism testing utilizing both adult plant and seedling testing to identify both qualitative and quantitative resistance loci will be necessary to sort out the allelic relationships among many of the QTL and Sr genes reported on wheat chromosomes.

In spite of the complexity of the meta-QTL analysis, using the available information on the Sr genes in the parents of biparental populations and accessions used for association mapping combined with the QTL location based on anchored markers, we were able to identify (a) QTL underlying some previously described Ug99 resistant Sr genes or residual effects of non Ug99 resistant genes and (b) putative locations of new Sr genes.

Colocation of Sr genes and QTL

Even though Sr31 carried by the rye introgression 1BL.1RS is not effective to Ug99, the three QTL found on chromosome 1BS, homoeologous to 1RS, are possibly due to a residual effect of Sr31 or another gene on the rye translocation because wheat cultivar PBW343 possesses Sr31. Additional studies are needed to validate whether the 1BS QTL are effects of the 1BL.1RS translocation or if there are one or more new APR genes on 1BS. Adult plant resistance gene Sr58 (Lr46/Yr29/Pm39) mapped distally on 1BL (McIntosh et al. 2012) and is independent of the seven QTL on chromosome 1BS. The response of Sr14 to Ug99 (Jin et al. 2007) was inconclusive, but because of its tetraploid origin and linkage to centromeric markers, the QTL on 1BS highlighted by Letta et al. (2013) is likely conferred by Sr14. Chromosome arm 2B, with 15 QTL, has the second highest number of QTL per chromosome arm, but also the highest number of known qualitative Ug99 Sr genes (6 numerically designated: Sr39, Sr32, Sr36, Sr40, Sr47, Sr28 and SrWeb). Sorting out the allelic relationships among these QTL and at least 11 qualitative Sr genes (seven Ug99 resistant and four non Ug99 resistant Sr genes) on chromosome 2B will be necessary to determine whether any new Sr genes have been detected or if they are the results of residual effects. The three QTL found in the AM durum panel (Letta et al. 2013) on chromosome 6AL overlap with Sr13, and even though Sr13 is not common in bread wheat, its presence in durum wheat suggests that the large region highlighted is most likely Sr13. Similarly, further studies are required to determine the allelic relationships among SrTA1662, SrCad, and Sr42 on 6DS (Hiebert et al. 2011; Ghazvini et al. 2012; Lopez-Vera et al. 2014). Chromosome 7B has nine QTL, eight of these QTL mapped near Sr17 (position not represented on Fig. 2) (Bansal et al. 2008). Though Sr17 is not effective to Ug99 in a qualitative manner (Jin et al. 2007) it is possible that Sr17 confers a residual APR effect. On chromosome 7D, early studies indicated that Sr57 (Lr34/Yr18/Pm38), Sr58 (Lr46/Yr29/Pm39) enhanced stem rust resistance in cultivar ‘Thatcher’ (Dyck and Kerber 1977; Kerber and Aung 1999), and later reports suggested that Sr57 provided APR to stem rust in diverse backgrounds (McIntosh et al. 2012). Our previous studies consistently showed that the STS marker csLV34 was significantly associated with Ug99 resistance in winter and spring CIMMYT wheat panels with major effects or through gene–gene interactions (Yu et al. 2011, 2012). Overlap of two QTL located distally on 7DL (Yu et al. 2011) are likely conferred by Sr25 (Ayala-Navarrete et al. 2007) because Sr43 and Sr44 are not currently used in wheat breeding.

Putative locations of new Sr genes

With six QTL found on 1A, that chromosome could be a potential new source of new APR. Because Sr1RS Amigo was introgressed from rye and confers qualitative resistance, it is most likely different from the six QTL on 1A. The same is true for the two QTL found on chromosome 1D. Even though they overlap with four described Ug99 resistant Sr genes (SrTA1662, Sr33, Sr45, Sr50: Rouse et al. 2011; Sambasivam et al. 2008; Anugrahwati et al. 2008), the Aegilops tauschii or rye origin of these genes make it unlikely that these QTL are related. The two QTL found on chromosome 2A are likely to represent new Ug99 Sr resistance loci because the only known gene in the region, Sr21 (The 1973), is derived from T. monococcum, and because the parents of the population with the 2A QTL (PBW343/Pavon76: CIMMYT unpublished) are not known to possess T. monococcum in their pedigrees. Also, Sr21 was not in the pedigrees of the AM durum panel (Letta et al. 2013). Chromosome group 3 has the second highest number of QTL, and chromosome 3B, with 23 QTL has the highest number of QTL by chromosome arm (Fig. 1). Chromosome arm 3BS has only one Ug99 Sr resistant gene, the slow rusting gene Sr2 and 19 of the QTL found on that arm are likely Sr2. In the Thatcher/McNeal population, a QTL was coincident with Sr12 on 3BL, a Ug99-ineffective resistance gene. It is possible that such defeated resistance genes could confer resistance when combined with genes such as Sr57 (Lr34/Yr18/Pm38) that have been demonstrated to confer epistatic resistance to stem rust (Kolmer et al. 2011; Rouse et al. 2014; Yu et al. 2012). Because Sr12 is present in the cultivar Thatcher and historically was used as an important source of resistance, Sr12 could be widespread in wheat germplasm. Since no resistance genes have been characterized on 3BL, the QTL in this region are conferred by one or more new resistance genes. Ug99 resistance gene Sr51 has been introgressed into translocations on each of the group 3 homoeologous chromosomes (Liu et al. 2011b). Since this gene is derived from Aegilops searsii and is not currently used in agriculture, none of the group 3 QTL are conferred by this gene. Chromosome 3A has three QTL, and the Sr35 cloned gene seems to overlap with the QTL in a CIMMYT population (CIMMYT unpublished) and in the durum panel (Letta et al. 2013) on chromosome 3AL, these QTL are not Sr35 because seedling resistance to race TTKSK is absent in the mapping population used by CIMMYT. In addition, the T. monococcum origin of Sr35 is absent from the pedigrees of the panel used by Letta et al. (2013). Resistance gene Sr27 is also on 3A, but this gene is different from these QTL because it is located on a rye introgression not present in the population used to identify these QTL. On Chromosome 4A, the stem rust resistance gene designated SrND643 (CIMMYT unpublished) is a qualitative gene and the PBW343/Juchi population does not possess that resistance gene so the QTL from PBW343/Juchi is likely conferred by a new resistance gene or allele. The relationships among the QTL identified on 4AL, SrND643, and Sr7 (Singh et al. 2006; Sears 1954) are not known. The distal end of chromosome 4B appears to be the source of a new Sr gene, although the variance explained for the QTL is low (9 %) and may represent a minor effect APR gene. Chromosome 5A does not carry any previously characterized Sr genes, so the three QTL identified on that chromosome arm likely represent new resistance loci. Although the phenotypic variation for the two QTL identified by Letta et al. (2013) on durum wheat is small (R 2 = 4.1 and 4.4 %), further studies of that region could support the existence of new Ug99 resistant loci. The APR gene Sr56 (McIntosh et al. 2012) on chromosome 5BL was previously designated QSr.Sun-5BL (Bansal et al. 2008) (not represented in Fig. 2). Among the 12 other QTL found on 5B, at least four are located on the short arm (Bansal et al. 2008; CIMMYT unpublished; Kaur et al. 2009; Yu et al. 2011) and likely represent new loci of importance. Although Kaur et al. (2009) did not test for APR to Ug99, Yu et al. (2011) also mapped distal 5BS QTL providing strong evidence for minor APR genes located in this region. Resistance gene Sr26 on 6AL is present in conventional common wheat germplasm from Australia, but was not present in CIMMYT germplasm when these studies were conducted. Therefore Sr26 does not explain the QTL identified through association mapping (Crossa et al. 2007; Yu et al. 2011). A putative new resistance QTL on 6AS was mapped in the hexaploid biparental PBW343/Kenya Swara population where no qualitative resistance genes are located (CIMMYT, unpublished). The QTL found on 6AL in the tetraploid population Kristal/Sebatel is likely a new source of resistance since Sr13-linked markers are in the distal region of 6AL. Chromosome 6B seems to be a rich source of new Sr genes with nine QTL detected. The relationship between the QTL on 6BL and Sr11 is not known but Sr11 is ineffective against Ug99 and is not represented on Fig. 2. Chromosome arm 6BS appears to be a new source of Sr genes (Crossa et al. 2007; Yu et al. 2011; CIMMYT unpublished). The phenotypic variation explained by these QTL ranged from 5 to 14 % and most likely correspond to minor APR genes. Singh et al. (2011) detected a distal QTL that explained 56 % of the variance for stem rust on chromosome 6BS (XwPt4283/XwPt7207) in the bi-parental cross between PBW343/Kenya Nyangumi. Validation of that QTL could be accomplished using the putative allele carrier, Kenya Nyangumi, in another bi-parental cross. The QTL on 7AS, either from durum or bread wheat, once validated, would represent new stem rust resistance genes as Sr22 is located on 7AL. Since Sr22 was not present in the parents of the hexaploid mapping populations, these QTL are not conferred by Sr22. The more distal QTL found in the Kristal/Sebatel bi-parental tetraploid population (QSr.1PK-7A.2) is likely to be conferred by Sr22 (Haile et al. 2012). Since Sr genes have not been previously identified on 7BS, the QTL identified in this region are likely new.

Overall, the growing number of characterized Sr genes and QTL demonstrates that there is still potential for discovering new APR genes with varying levels of effect. This consensus map will facilitate the identification of new resistance genes and QTL and aid in the development of improved markers to increase breeding efficiency and the pool of alleles that are important for the control of Ug99.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 560 kb) (560.5KB, pdf)

Conflict of interest

None of the people involved in this publication have a conflict of interest with the content presented.

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