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. 2014 Jun 3;53(24):3971–3981. doi: 10.1021/bi500428j

Figure 4.

Figure 4

Activation of PHLPP by DTT and selected lipophilic compounds. (a) Effect of DTT on PHLPP1 and PHLPP2 activity. k values for PHLPP1 (white bars) and PHLPP2 (gray bars) were determined at 10 mM pNPP, in the absence of Mn2+ for different concentrations of DTT. (b) Effect of different lipophilic compounds on PHLPP2 phosphatase activity. Reactions were conducted in Tricine (pH 7.5) at 10 mM pNPP and 1 mM MnCl2 in the presence of 100 μM lipophilic compounds. (c) Relative activity of the PHLPP2 phosphatase domain with increasing concentrations of oleic (circles) and arachidonic (triangles) acid. Reactions were conducted in Tricine (pH 7.5) at 10 mM pNPP and 5 mM MnCl2. Graphs show means ± SEM for three separate experiments. *P < 0.01, and **P < 0.001 (Student’s t test).