Human IGF1 Regulates Midgut Oxidative Stress and Epithelial Homeostasis to Balance Lifespan and Plasmodium falciparum resistance in Anopheles stephensi

Abstract

Insulin and insulin-like growth factor signaling (IIS) regulates cell death, repair, autophagy, and renewal in response to stress, damage, and pathogen challenge. Therefore, IIS is fundamental to lifespan and disease resistance. Previously, we showed that insulin-like growth factor 1 (IGF1) within a physiologically relevant range (0.013–0.13 µM) in human blood reduced development of the human parasite Plasmodium falciparum in the Indian malaria mosquito Anopheles stephensi. Low IGF1 (0.013 µM) induced FOXO and p70S6K activation in the midgut and extended mosquito lifespan, whereas high IGF1 (0.13 µM) did not. In this study the physiological effects of low and high IGF1 were examined in detail to infer mechanisms for their dichotomous effects on mosquito resistance and lifespan. Following ingestion, low IGF1 induced phosphorylation of midgut c-Jun-N-terminal kinase (JNK), a critical regulator of epithelial homeostasis, but high IGF1 did not. Low and high IGF1 induced midgut mitochondrial reactive oxygen species (ROS) synthesis and nitric oxide (NO) synthase gene expression, responses which were necessary and sufficient to mediate IGF1 inhibition of P. falciparum development. However, increased ROS and apoptosis-associated caspase-3 activity returned to baseline levels following low IGF1 treatment, but were sustained with high IGF1 treatment and accompanied by aberrant expression of biomarkers for mitophagy, stem cell division and proliferation. Low IGF1-induced ROS are likely moderated by JNK-induced epithelial cytoprotection as well as p70S6K-mediated growth and inhibition of apoptosis over the lifetime of A. stephensi to facilitate midgut homeostasis and enhanced survivorship. Hence, mitochondrial integrity and homeostasis in the midgut, a key signaling center for IIS, can be targeted to coordinately optimize mosquito fitness and anti-pathogen resistance for improved control strategies for malaria and other vector-borne diseases.

Author Summary

The complexity of the malaria parasite life cycle makes it an elusive target for drug and vaccine development. Thus, targeting the parasite in the mosquito vector is an attractive alternative. When consuming an infective blood meal the mosquito ingests not only the blood proteins and parasites, but a range of host blood factors, including the insulin-like growth factor-1 (IGF1) hormone. IGF1 is a highly conserved signaling molecule that regulates a broad spectrum of cellular processes, including immunity and midgut homeostasis. We previously demonstrated that human IGF1 ingested in a blood meal can induce cell signaling in the mosquito midgut that reduces malaria parasite development and extends mosquito lifespan. In this study, we show that midgut signaling by human IGF1 increased the synthesis of reactive oxygen species in midgut mitochondria and enhanced nitric oxide synthase gene expression, responses that inhibit malaria parasite development in the mosquito. Additionally, we found that IGF1 signaling facilitates midgut homeostasis to enhance mosquito survival. These results suggest that IGF1 signaling in the mosquito midgut could be targeted to coordinately enhance mosquito fitness and anti-parasite resistance for improved malaria control strategies.

Introduction

More than fifty percent of the world's population is currently at risk for malaria infection. Plasmodium falciparum, the most virulent of the human malaria parasites, infects approximately 250 million people each year [1], a disproportionate number of whom are children under age five. Current control measures include drugs and insecticides, but increased prevalence of resistant parasite strains and mosquitoes [2] has necessitated the development of novel control strategies, including use of genetically modified mosquitoes, to combat this disease.

The definitive hosts for anthroponotic species of Plasmodium are mosquitoes in the genus Anopheles. Parasites undergo rapid developmental transitions in the midgut while mosquito immune barriers function coordinately to limit infection [3], [4]. Primary physical and chemical barriers of the midgut, including increased levels of reactive oxygen species (ROS) and nitric oxide (NO) kill parasites in the gut lumen, preventing contact between parasites and the epithelium [5]–[10]. Secondary defenses are activated when parasites encounter the midgut epithelium and include the production of nuclear factor (NF)-κB-dependent immune effectors such as antimicrobial peptides (AMPs) and complement-like factors which together function to further limit parasite development [3], [4]. Additionally, human hormones, cytokines, and growth factors that are ingested with the infectious blood meal can regulate mosquito physiology to affect parasite development [11]. For example, we have shown that the Indian malaria mosquito Anopheles stephensi can respond to ingested human insulin [12]–[14]. Insulin can rise to high levels in blood during malaria infection [15], [16] and, when ingested during mosquito feeding, activates canonical insulin/insulin-like growth factor signaling (IIS) in the midgut to reduce mosquito lifespan and increase P. falciparum infection [12]–[14]. A. stephensi can also respond to ingested human IGF1 [17], which unlike insulin, declines in serum during malaria [18]. In particular, levels of IGF1 in blood of healthy humans can fall from 0.09 µM [19], [20] to below 0.006 µM during severe infections with P. falciparum and Plasmodium vivax [18]. To understand the impact of ingested human IGF1 on A. stephensi, we examined the effects of 0.013 µM IGF1 (physiologically low) and 0.13 µM IGF1 (physiologically high) on mosquito lifespan and P. falciparum infection. Intriguingly, we noted that both concentrations provided in a blood meal reduced P. falciparum infection, while low IGF1 significantly extended lifespan relative to high IGF1-fed mosquitoes, which were not significantly different from controls [17].

The effects of insulin and IGF1 on IIS in A. stephensi are distinct and likely dictate the markedly different effects of these growth factors on lifespan and infection. In particular, human insulin induces phosphorylation of FOXO in the A. stephensi midgut [13] as does low IGF1 [17], whereas high IGF1 has no effect on FOXO [17]. Low IGF1 induces phosphorylation of p70S6K, a protein that is not activated by insulin, and both low and high IGF1 repress phosphorylation of ERK [17], a protein that is activated by insulin in the A. stephensi midgut [12], [13]. Given that IGF1 regulation of p70S6K is crucial to inhibition of BCL2-mediated apoptosis [21], we speculated that lifetime treatment of A. stephensi with a low concentration of IGF1 may optimally regulate apoptosis for midgut homeostasis, resulting in longer-lived mosquitoes.

A significant body of literature points to IIS regulation of epithelial homeostasis through a balance of death (apoptosis), survival (autophagy) and renewal (stem cell proliferation and differentiation) of epithelial cells to define longevity and disease resistance. For example, when the D. melanogaster midgut is damaged, enterocytes are extruded into the D. melanogaster gut lumen and display both increased caspase-3 activity, indicating an irreversible commitment to apoptotic cell death, and hallmarks of autophagy, including histological evidence of autophagosomes and enhanced gene expression of autophagy related gene 8 (ATG8) [22]. Autophagy can modulate lifespan and senescence [23] and several studies have shown connections among IIS, autophagy, and pathogen resistance as well. In C. elegans, pathogen resistance and lifespan extension are dependent on autophagy gene expression in IIS-inhibited Daf-2 mutants [24], [25]. A number of recent studies have also shown that IIS in D. melanogaster can alter lifespan through complex effects on gut epithelial renewal [26]. Long-lived IIS mutants are surprisingly deficient in proliferative responses to tissue damage [27], yet exhibit enhanced pathogen resistance [28], [29]. Moderate reduction in IIS in intestinal stem cells (ISCs), in contrast, can reduce cell proliferation and extend Drosophila lifespan, while inhibition of IIS in ISCs impaired midgut renewal and shortened lifespan, indicating that an optimal level of IIS is necessary to regulate gut epithelial proliferation and repair, lifespan, and host defense [30].

In A. stephensi, enhanced IIS via Akt overexpression in the midgut reduced expression of genes associated with autophagy (ATG6, ATG8) and epithelial renewal (Esg, Pros), resulting in the accumulation of dysfunctional mitochondria and toxic reactive nitrogen and oxygen species (RNOS) that damaged the midgut and shortened mosquito lifespan [31]. Conversely, when the IIS antagonist phosphatase and tensin homolog (PTEN) was overexpressed to repress IIS in the mosquito midgut, increased autophagy and enhanced expression of markers of epithelial renewal were observed in concert with extended lifespan and enhanced pathogen resistance [32]. From our own observations as well as studies of model organisms, we have inferred that IIS is a central regulator of gut epithelial homeostasis in A. stephensi. Further, this biology is mediated by changes in RNOS, mitochondrial integrity, and stem cell maintenance and differentiation, which can coordinately modulate lifespan and pathogen resistance [31]. Here, we sought to define IIS-dependent biological processes that define IGF1 phenotypes in A. stephensi. To this end, we examined outputs of NF-κB-dependent immunity in the context of key parameters of gut epithelial homeostasis in A. stephensi to define IGF1-specific features of longevity and parasite resistance.

Results

Human IGF1 induced JNK activation, but not NF-κB-dependent immune responses, in A. stephensi

We have previously shown that ingested human insulin inhibits NF-κB-dependent immunity in A. stephensi leading to increased malaria parasite development [13], [14]. Therefore, we reasoned that ingested IGF1 might activate NF-κB-dependent immunity to inhibit P. falciparum development in the mosquito. In addition to IIS regulation, IGF1 can potently activate the mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK) via receptor-interacting protein (RIP) [33]. The RIP family proteins are crucial mediators for multiple inputs that activate NF-κB-dependent responses [34] and encode death domains orthologous to those of immune deficiency (imd) [35], a major regulator of NF-κB-dependent immunity in D. melanogaster [36] and Anopheles mosquitoes [37]. Given that Plasmodium berghei killing in Anopheles gambiae has been associated with JNK activation [38] and that JNK functions cooperatively with NF-κB mediated defenses downstream of TAK1 activation [39]–[42], we reasoned that IGF1 might activate A. stephensi JNK to upregulate NF-κB-dependent and/or imd-associated anti-parasite responses. We first assessed the effects of IGF1 treatment on midgut levels of JNK activation in A. stephensi using phospho-specific antisera and observed that only low concentrations of IGF1 significantly induced JNK activation in the A. stephensi midgut relative to controls at 30 min post-feeding (Figure 1). While relative induction levels of low and high IGF1 were not significantly different from one another, high IGF1 showed only a trend towards moderately increased JNK activation (20%) relative to controls (Figure 1).

Figure 1. Ingested human IGF1 increased JNK phosphorylation in A. stephensi midguts.

Mosquitoes were fed blood meals containing 0.013 µM or 0.133 µM IGF1 or an equivalent volume of PBS. Midguts were dissected at 0.5–1 h after the initiation of blood feeding. Data are represented as the average fold induction±SEM of phospho-JNK quantified by densitometry and normalized first to the GAPDH loading control and then to the buffer control. A representative western blot is shown below the graph. Data were analyzed by Mann Whitney test. Experiments were replicated four times and p-values are displayed on the graphs.

We next examined the effects of IGF1 treatment on induction of NF-κB-dependent responses in A. stephensi cells in vitro and in vivo. For in vitro assays, A. stephensi embryonic (ASE) cells were transfected with plasmids with NF-κB-dependent promoters from Defensin or Gambicin genes fused to a luciferase reporter gene as described [14], then stimulated with lipopolysaccharide (LPS) with or without 0.013 or 0.13 µM IGF1 and assayed for luciferase activity. Neither concentration of IGF1 affected promoter-reporter activity relative to treatment with LPS alone (Figure 2A, Figure S1), suggesting that IGF1 does not induce NF-κB-dependent gene expression in A. stephensi cells. We next assessed the effects of IGF1 on the expression of imd-associated immune effector genes APL1, LRIM, TEP1, and LRRD7 [37] in vivo using quantitative RT-PCR (Figure 2B). We have shown previously that A. stephensi fed artificial blood meals supplemented with freeze-thaw P. falciparum parasite products (FTPP) have increased expression of immune genes in the midgut epithelium 24 h post-blood feeding (9). Therefore, we fed female A. stephensi blood meals supplemented with FTPP and 0.013 µM or 0.13 µM IGF1 or FTPP and an equivalent volume of PBS (diluent for IGF1) as a control. IGF1 in the physiologically low range (0.013 µM) did not alter expression of these immune genes at 24 h post-feeding and a higher physiological concentration of IGF1 (0.13 µM) markedly reduced APL1 and TEP1 transcript levels despite activation of JNK (Figure 1). Therefore, IGF1 did not induce NF-κB-dependent promoter activity in A. stephensi cells in vitro (Figure 2A, Figure S1) and showed a trend towards repressed expression of imd-associated complement-like immune factors in the midgut in vivo (Figure 2B).

Figure 2. IGF1 did not induce prototypical NF-κB-dependent immune responses in A. stephensi cells in vitro (A) or in vivo (B).

(A) Human IGF1 did not alter antimicrobial peptide (AMP) promoter activity in immune-activated ASE cells. Graphs depict fold change in relative light units (RLU) from promoter-reporter assays in transfected cells stimulated with or without LPS and with or without IGF1. Promoter activities following treatment were normalized first to levels in matched controls without LPS treatment, then to levels in matched controls treated with LPS and without IGF1 to define IGF1-specific changes in promoter activity. Data were analyzed by unpaired t-tests comparing each treatment to the matched buffer control; NS indicates p>0.1. Defensin n = 4; Gambicin n = 3. (B) Ingested human IGF1 reduced or had no effect on expression of immune effector genes APL1, TEP1, LRIM and LRRD7. Mosquitoes were fed artificial blood meals that were supplemented with P. falciparum FTPP and treated with 0.013 or 0.13 µM IGF1 or an equivalent volume of PBS as a control. Midguts were dissected 24 h after the initiation of blood feeding and RNA isolated for quantitative RT-PCR. Data are represented as fold change (2^−ΔΔCt) in immune gene expression in FTPP-treated A. stephensi relative to matched control (PBS). Data were analyzed using Kruskal-Wallis non-parametric tests followed by Dunn's post-tests. Experiments were replicated six times with separate cohorts of mosquitoes and p-values are indicated.

Human IGF1 increased midgut RNOS in A. stephensi

JNK activation has also been linked to regulation of heme peroxidase 2 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 5, which have been reported to function with NOS to opsonize parasites through TEP1-mediated lysis in A. gambiae [43]. Additionally, repression of midgut ERK phosphorylation by low and high IGF1 [17] could also act to enhance midgut NOS expression [8]. Together, these observations suggested that IGF1-mediated JNK activation and ERK repression could coordinately fuel RNOS synthesis and NO-dependent parasite killing in A. stephensi. To determine whether human IGF1 increased midgut RNOS, we quantified soluble peroxides, mitochondrial superoxide, and NOS gene expression levels in dissected midgut tissue following treatment with low (0.013 µM) and high (0.13 µM) IGF1.

At 6 h post-treatment, high IGF1 significantly increased midgut peroxides, and by 24 h, both low and high IGF1 treatments increased midgut peroxide levels relative to controls (Figures 3A, B). Superoxide generated by mitochondria as a consequence of oxidative phosphorylation is the major cellular source of these ROS and superoxide dismutase converts mitochondrial superoxide to hydrogen peroxide [44], which can freely diffuse out of mitochondria. To determine whether the observed increases in midgut soluble peroxides originated from increased mitochondrial superoxide, we quantified superoxide levels in the mitochondria using MitoSOX Red, a selective fluorogenic dye that reacts specifically with mitochondrial superoxide [45]. At 6 h following IGF1 treatment, confocal microscopy of stained midguts revealed punctate MitoSOX Red staining that was consistent with mitochondrial superoxide localization (Figure 3C; merged images). This staining was significantly increased above control by treatment with 0.013 µM IGF1 to levels that were comparable to treatment with rotenone, an inducer of mitochondrial superoxide production [44] (Figure 3D). Taken together, IGF1 increased midgut mitochondrial superoxide and peroxides within 24 h of treatment, a possible consequence of IGF1-induced mitochondrial energy biosynthesis [46].

Figure 3. Ingested human IGF1 increased ROS levels in A. stephensi midguts.

Peroxide levels were quantified at 6(A) and 24 h (B) following IGF1 treatment. Each data point represents the quantified peroxides in a pool of five midguts; three pools were collected per treatment per experiment. Data are represented as fold-change relative to saline/ATP-fed controls and were analyzed by unpaired t-test. Experiments were replicated three times with separate cohorts of mosquitoes and p-values are indicated. (C) Representative images from confocal microscopy of MitoSOX Red stained midguts. Upper Panel: MitoSOX Red staining, scale bars = 20 µm; Middle Panel: DAPI staining scale bars = 20 µm; Lower Panel: Merged images, scale bars = 10 µm. Differences in nuclei size are due to differences in focal plane not magnification. (D) Mitochondrial superoxide levels were quantified from MitoSOX Red fluorescence. Average fold inductions±SEM of MitoSOX Red fluorescence from low IGF1 (0.013 µM) and a positive control (1 µM rotenone) relative to controls. Data were analyzed by unpaired t-test (n = 4).

To determine whether human IGF1 concurrently increased midgut NOS levels, gene expression was quantified in midguts from A. stephensi females fed on artificial blood meals supplemented with low or high IGF1 (0.013 or 0.13 µM) or supplemented with an equivalent volume of PBS as a control at 6 h and 24 h following blood feeding. NOS expression was significantly higher in midguts of mosquitoes fed low and high IGF1 at 6 h post-blood meal (PBM) compared with blood-fed controls (Figure 4A). At 24 h PBM, NOS gene expression remained elevated in low and high IGF1-fed mosquitoes relative to controls, but variability in expression was greater at this timepoint (Figure 4B). Together with elevated levels of ROS (Figure 3), these data suggested that IGF1 stimulates the production of RNOS, which could underlie IGF1-mediated inhibition of parasite development [17].

Figure 4. Ingested human IGF1 increased NOS gene expression in A. stephensi midguts.

Mosquitoes were fed artificial blood meals supplemented with 0.013 or 0.13 µM IGF1 or with an equivalent volume of PBS as a control. Midguts were dissected at (A) 6 h and (B) 24 h after feeding and RNA was isolated for quantitative RT-PCR. Data are represented as fold change in NOS gene expression relative to PBS control. Experiments were replicated 4–6 times with separate cohorts of mosquitoes and p-values are indicated. Normally distributed data were analyzed by unpaired t-test (24 h), and non-normally distributed (6 h) data were analyzed by Mann Whitney test.

NOS inhibition reversed IGF1-induced resistance to P. falciparum

To assess whether RNOS were responsible for IGF1-induced resistance to P. falciparum, four separate cohorts of female A. stephensi were provided with water alone or water supplemented with 3.7 µM Nω-Nitro-L-arginine methyl ester (L-NAME), an inhibitor of NOS catalysis, for 72 h preceding infection. At the time of P. falciparum infection, mosquitoes were provided identical parasite-infected meals supplemented with or without 0.13 µM IGF1 and with or without 3.7 µM L-NAME. After feeding, infected mosquitoes were maintained on water or water with L-NAME as appropriate until dissection. At 10 days post-infection, L-NAME treatment in the absence of IGF1 increased infection prevalence (proportion of mosquitoes infected with at least one P. falciparum oocyst; Figure 5A) and intensity (mean oocysts per midgut; Figure 5B) relative to controls, consistent with previous observations [47]. As observed previously [17], treatment with IGF1 significantly reduced both prevalence and intensity of P. falciparum infection relative to control (Figures 5A, B). The addition of L-NAME to IGF1 treatment, however, reversed the phenotype of IGF1-associated infection resistance (IGF1+L-NAME vs IGF1: prevalence p = 0.05, intensity p = 0.003), resulting in prevalence of infection (Figure 5A) and mean oocysts per midgut (Figure 5B) in L-NAME+IGF1 treated mosquitoes that were not significantly different from control (IGF1+L-NAME vs control: prevalence p = 0.12, intensity p = 0.38).

Figure 5. IGF1-induced resistance to P. falciparum was reversed by NOS inhibition.

Age- and cohort-matched mosquitoes were fed infected blood meals supplemented with water, L-NAME, 0.13 µM IGF1 or 0.13 µM IGF1+L-NAME as described in Materials and Methods. This experiment was replicated four times with separate cohorts of mosquitoes. No significant differences in oocyst intensity were identified among replicate control groups (Kruskal-Wallis, p>0.05), so data were pooled for analysis. Intensity data shown are from infected midguts only. (A) Prevalence of infection (proportion of mosquitoes dissected with at least one P. falciparum oocyst; values indicated above bars). Fisher's exact test was used to compare treatments against L-NAME. (B) Intensity of infection (mean oocysts per infected midgut; values indicated to the right of data for each group). Mann-Whitney test was used to compare treatment groups to matched controls.

IGF1-induced RNOS are associated with enhanced caspase-3 activity and functional changes to the midgut epithelial barrier

Our results indicate that IGF1-induced RNOS, rather than enhanced NF-κB-dependent innate immune responses, kill malaria parasites in the midgut of A. stephensi. While this may be beneficial for control of infection, sustained high levels of NO and ROS can also directly damage the gut epithelium and destabilize cellular junctions, which are critical to epithelial barrier function [31]. Based on these observations and our previous findings that IGF1 can extend lifespan in A. stephensi [17], we sought to assess midgut peroxides and epithelial integrity at extended times post-feeding of low (0.013 µM) and high (0.13 µM) IGF1. We observed that peroxides were significantly elevated by both treatments relative to control at 24 h (Figure 3B); at 72 h, only high IGF1 induced significant induction of peroxides relative to control (Figure 6A, Figure S2A). In addition, we assayed caspase-3 activity, a critical determinant of apoptotic regulation of epithelial barrier integrity [48]–[50], in midgut samples at 72 h. Caspase-3 activity was enhanced in both IGF1 treated groups compared to controls at 72 h (Figure 6B, Figure S2B), likely a reflection of the trend toward elevated peroxides with low IGF1 treatment and significant induction of peroxides with high IGF1 treatment at this timepoint (Figure 6A, Figure S2A). Elevated caspase-3 activity in midguts from both treatment groups suggested the occurrence of damage that could alter the physical barrier integrity of the midgut. Indeed, when we measured barrier integrity as the transit of fluorescent beads through the midgut at 72 h post-feeding, body bead counts (whole mosquito beads minus midgut beads) from both IGF1 treatment groups were increased significantly relative to controls (Figure 6C). Hence, IGF1-induced midgut ROS synthesis through 72 h was associated with an upregulation of caspase-3 activity and tissue damage as evidence by increased midgut permeability.

Figure 6. Midgut peroxides and epithelial integrity were altered at 72-IGF1 feeding.

Peroxide levels in midguts at 72(A) and one week (D) following IGF1 treatment. Peroxides were quantified in pools of five midguts; three pools were collected per treatment per experiment. Experiments were replicated three times with separate cohorts of mosquitoes. Graphs show the mean fold induction of peroxides±SEM in IGF1 treated groups relative to saline/ATP-fed controls. Statistical significance was determined by unpaired t-test and p-values are indicated. Midgut caspase-3 activity at 72 h (B), one week (E), two weeks (F), and three weeks (G) following IGF1 treatment. Graphs show the average fold induction±SEM of AFC fluorescence (caspase-3 activity) relative to controls. Three pools of five midguts per treatment were collected for 72 h and one week timepoints and 1–2 pools were collected for two and three week timepoints. Experiments were replicated 4–6 times with separate cohorts of mosquitoes. Caspase-3 activity among groups was compared using Kolmogorov-Smirnov test. (C) Midgut permeability at 72 h following a blood-meal containing IGF1 and 3–3.5 µm fluorescent beads (Spherotech). Each data point represents the number of fluorescent beads in three whole mosquitoes quantified by flow-cytometry. Statistical significance was determined by one-way ANOVA followed by Fisher's LSD.

Our previous studies reported that survivorship of 0.013 µM IGF1-fed mosquitoes was greater than that of 0.13 µM IGF1-fed mosquitoes and untreated controls beginning at two weeks post-treatment. This divergent pattern was sustained for the duration of mosquito lifespan [17], suggesting that the effects of IGF1 on lifespan derive from distinct temporal effects of IGF1 on midgut homeostasis. This inference was supported by our data. In particular, midgut peroxide levels at one week post-feeding (Figure 6D, Figure S2C) remained significantly higher relative to controls in 0.13 µM IGF1-fed mosquitoes, while levels in 0.013 µM IGF1-fed mosquitoes had returned to baseline. Further, by one week post-feeding, midgut caspase-3 activity closely mirrored midgut peroxide levels in both IGF1 treatment groups (Figure 6E, Figure S2D), suggesting a link between the transient ROS produced in response to physiologically low levels of IGF1 and mechanisms of damaged midgut repair. In contrast, caspase-3 activity was sustained up to 3 weeks post-treatment with high IGF1 (Figures 6F and G, Figures S2E and F), suggesting that a lack of enhanced survivorship in this treatment group [17] resulted from sustained midgut damage.

IGF1 regulates autophagy and ISC homeostatic processes in a concentration dependent manner

Several studies have suggested that autophagic repair is necessary for optimal stem cell maintenance and differentiation [51], [52]. These processes, in turn, facilitate enhanced pathogen resistance [25], [53], [54] and epithelial barrier integrity and repair functions in the gut [55], [56]. To determine whether the IGF1 phenotypes we observed were associated with expression patterns typical of ISC proliferation and differentiation we examined transcript levels for Esg (proliferation) and Pros (differentiation) over time in the midgut epithelium of A. stephensi [31], [57], [58] fed artificial blood meals supplemented with low and high IGF1 or supplemented with an equivalent volume of PBS as a control. To monitor the formation and maturation of autophagosomes, we also examined transcript levels of ATG6 (autophagosome initiation; [59]) and ATG8 (maturation of the autophagosomal membrane; [59]) as previously described [31].

Esg expression was significantly decreased relative to control at 24 h (p = 0.0003), but levels remained relatively unchanged from control at 72 h and one week post-treatment (Table 1). From 24 h through one week post-treatment with low IGF1, Pros expression levels were relatively unchanged from control (Table 1). In contrast, treatment with high IGF1 was associated with significant increases of 11-fold for Esg (p = 0.0003) by 72 h and 74-fold for Pros by one week (p = 0.002) relative to control (Table 1). These data suggested that enteroblast proliferation and differentiation are maintained at steady-state levels by low IGF1 treatment, while high IGF1 treatment was associated with a marked rise in proliferation and differentiation starting at 72 h PBM. Autophagy biomarker gene expression patterns were also distinct between these two IGF1 treatments. In particular, low IGF1 treatment induced moderate increases (2-fold relative to control) in ATG6 expression at one week post-treatment (Table 1; p = 0.06) and a greater than 19-fold increase in ATG8 expression relative to control at 24 h (p = 0.02) that was not sustained at 72 h and one week post-treatment (Table 1). Treatment with high IGF1 moderately increased ATG6 expression at 72 h post-treatment (p = 0.06, Table 1). However, ATG8 expression levels with high IGF1 treatment differed from that with low IGF1 in that a smaller significant induction (4.65-fold, p = 0.03) relative to control was noted at 72 h (Table 1), and by one week post-treatment, ATG8 expression levels were only 29% of control levels (p = 1.62E-07, Table 1). These data suggested that maturation of autophagosomes was enhanced early and significantly by low IGF1, whereas this process was transiently enhanced at 72 h and then significantly repressed by high IGF1 by one week post-treatment.

Table 1. IGF1 regulates autophagy and ISC homeostatic processes in a concentration dependent manner.

	0.013 µM IGF1			0.13 µM IGF1
	24 h	72 h	1 wk	24 h	72 h	1 wk
Esg	0.32	2.62	0.61	0.20	11.12	2.11	geometric mean
	0.0003	0.44	0.25	0.35	0.0003	0.20	p-value
Pros	1.31	7.88	2.22	1.92	2.00	74.39	geometric mean
	0.19	0.17	0.87	1.00	0.99	0.002	p-value
ATG6	0.76	3.36	2.06	2.58	2.22	3.83	geometric mean
	0.49	0.33	0.06	0.23	0.06	0.15	p-value
ATG8	19.75	6.53	0.11	14.01	4.65	0.29	geometric mean
	0.02	0.99	1.00	0.26	0.03	1.62E-07	p-value

Gene expression levels were calculated using the 2^−ΔΔCt method relative to expression of the ribosomal protein s7 (RPS7) gene. Values indicate geometric mean fold changes between IGF1-fed and buffer-fed controls. Bold values indicate significant (p<0.05) or nearly significant (p = 0.06) fold changes in expression relative to control.

The beneficial effects of low dose IGF1 on A. stephensi survivorship was ablated by enhanced oxidative stress

Our data suggest that low IGF1 treatment induces transient oxidative stress and caspase-3 activity (Figure 6) as well as early enhanced autophagy and maintenance of homeostatic levels of epithelial proliferation and differentiation (Table 1) that are consistent with lifespan extension [17]. Conversely, high IGF1 treatment induced prolonged oxidative stress (Figure 6), sustained midgut caspase-3 activity (Figure 6), and aberrant expression patterns of epithelial cell proliferation, differentiation, and autophagy biomarkers (Table 1). These data suggested that the lack of lifespan extension observed in high IGF1 treated mosquitoes was due a reduced capacity to recover from oxidative stress. To address this hypothesis, 3–5 day old female A. stephensi were provided reconstituted human blood meals supplemented with 1 mM paraquat, an inducer of mitochondrial ROS [44], 1 mM paraquat and low or high IGF1, or supplemented with diluent at volumes equivalent to those of the treatment groups. Because paraquat acutely induces oxidative stress, our observations were temporally restricted to 72 h post-treatment, a time at which midgut peroxides following low IGF1 treatment were no longer significantly elevated relative to controls (Figure 6). We expected that capacity for recovery from oxidative stress within the period of observation, therefore, would be greater in the low IGF1 treatment group. However, the addition of low and high IGF1 to paraquat treatment resulted in nearly identical reductions in survivorship relative to paraquat alone (Figure 7). These observations suggested that the beneficial effects of low IGF1 on lifespan extension of A. stephensi were not due to a greater capacity for recovery from oxidative stress, which would mitigate paraquat-induced stress, but rather to the lower levels of midgut epithelial ROS induced by low versus high IGF1.

Figure 7. Effect of low dose IGF1 on A. stephensi survivorship was ablated by enhanced oxidative stress.

Mosquitoes were fed saline/ATP supplemented with: (1) low (0.13 µM) IGF1 or high (0.13 µM) IGF1 and 1 mM paraquat (P), (2) an equivalent volume of PBS and 1 mM paraquat (PBS+P), or (3) an equivalent volume of PBS without paraquat (PBS−P) as an untreated control. Dead mosquitoes were removed and counted at intervals between 8 h and 72 h. The experiment was replicated twice with separate cohorts of mosquitoes. Survival analyses were performed using the Kaplan Meier method and differences between survival curves calculated using the Wilcoxon test. Survival curves for 0.13 µM IGF1+P and 0.13 µM IGF1+P were not significantly different from each other and neither was significantly different from PBS+P (p>0.05). Survival curves for low IGF1+P, high IGF1+P, and PBS+P, however, were significantly different from the untreated control PBS−P (p<0.05).

Discussion

Previously, we showed that physiological concentrations of human IGF1 reduced P. falciparum development in A. stephensi, and that low IGF1 (0.013 µM) extended mosquito lifespan while high IGF1 (0.13 µM) did not [17]. By extension, mechanisms of parasite killing should be shared by low and high IGF1 concentrations, while lifespan extending effects of IGF1 should be unique to low concentrations of this growth factor. We focused our studies accordingly and assert that our observations not only explain these divergent effects of IGF1, but also extend our understanding of the intersection of lifespan and pathogen resistance as mediated by midgut IIS.

Here we have demonstrated that both low and high IGF1 induced midgut NOS expression at 6 h and 24 h post-feeding (Figure 4), although low IGF1 may have done this more effectively, via enhanced JNK activation, than high IGF1 treatment (Figure 1). Regardless, inhibition of NOS activity by L-NAME reversed IGF1 inhibition of P. falciparum development (Figure 5). Parasite killing, therefore, is mediated through toxic RNOS [7] formed in the presence of IGF1-induced NO and ROS in the midgut lumen. Hence, although JNK activation may mediate anti-parasite defenses beyond those examined here, their contribution is clearly masked by a dominant NO-mediated defense.

While the mechanism of IGF1-mediated control of parasite infection was reasonably clear, our data suggested that the divergent effects of low and high IGF1 on A. stephensi survivorship and lifespan were more complex. Key observations included enhanced JNK activation that was specific to low IGF1 (Figure 1), significant inductions in mitochondrial ROS and peroxides produced in the midgut that were sustained only with high IGF1 (Figures 3, 6), and aberrant expression patterns of epithelial cell proliferation, differentiation, and autophagy biomarkers with high IGF1 treatment (Table 1). We also observed high IGF1-induced midgut caspase-3 activity for up to 3 weeks post-treatment (Figure 6), a time during which differences in survivorship of A. stephensi treated with low and high IGF1 were significant [17].

Importantly, these phenotypes correspond to the dichotomy in IGF1-stimulated phosphorylation of IIS proteins, namely, that only low IGF1 induced phosphorylation of FOXO and p70S6K in A. stephensi midguts [17]. We propose that FOXO and p70S6K activation induce cytoprotective responses that ameliorate damage and protect and expand mitochondrial function, resulting in increased mosquito lifespan. In particular, activation of p70S6K is critical to inhibiting BCL2-mediated apoptosis [21], which could explain the attenuation of midgut caspase-3 activity observed in A. stephensi following low dose IGF1 treatment. Further, only low IGF1 significantly activated JNK, which in turn could enhance pleiotropic cytoprotection beyond JNK-mediated midgut host defense, including targeting of Akt-phosphorylated FOXO to activate an antioxidant program that reduces cellular ROS [60], [61]. JNK-mediated cytoprotection would also be consistent with homeostatic ISC biomarker expression in the A. stephensi midgut following low IGF1 treatment. In Drosophila, ISCs are mostly quiescent and become highly proliferative following injury [30], [62]. In contrast, midgut epithelium from A. stephensi treated with low IGF1 showed a small but significant change in Esg expression, suggesting that midgut cells experience little, if any, injury from enhanced RNOS. Over the lifetime of A. stephensi, patterns of transient ROS induction coupled with early epithelial recovery could facilitate midgut homeostasis that would be consistent with enhanced survivorship in the context of parasite killing (Figure 8).

Figure 8. Proposed model: IGF1 fine-tunes the balance of epithelial homeostasis and midgut integrity, enhancing survival and anti-parasite resistance in A. stephensi.

Human IGF1 in the midgut initiates signaling cascades that repress phosphorylation of ERK, enhancing midgut RNOS that induce parasite death. Low IGF1 acts through FOXO, JNK and P70S6K to induce JNK/IIS-mediated cytoprotective responses that moderate levels of ROS and NO to initiate early mitophagy, transient apoptosis, and ISC homeostasis, which increase mosquito survivorship. In contrast, high IGF1 does not activate JNK/IIS compensatory signaling, resulting in delayed autophagy and sustained RNOS, which drive damage-associated apoptosis and midgut epithelial dysplasia.

The possibility that transient levels of ROS are beneficial to A. stephensi is supported by recent observations in D. melanogaster. In particular, Owusu-Ansah et al. [63] showed that moderate levels of oxidative stress in muscle mitochondria induced mitohormesis or adaptive cytoprotective responses that preserve mitochondrial function and extend lifespan in D. melanogaster. Importantly, these protective effects were due to the actions of (i) JNK, which induced the mitochondrial unfolded protein response (UPR) preserving mitochondrial function and (ii) induction of ImpL2 (the Drosophila homologue of IGF-binding protein 7), which enhanced muscle mitophagy and repressed systemic insulin signaling, preserving tissue integrity and contributing to extended lifespan. These observations suggest that JNK-mediated feedback to IIS could reduce mitochondrial ROS to “optimal” levels in the mosquito midgut in response to low IGF1 treatment, which would promote UPR and preserve mitochondrial integrity. Early induction of ATG8 would also facilitate timely removal of moderately dysfunctional mitochondria, thus protecting the pool of optimally functioning organelles. Finally, while insulin-like peptide (ILP) binding proteins have yet to be identified in A. stephensi, proteins that can bind to mosquito ILP3 and 4 have been identified in Aedes aegypti [64], suggesting that ImpL2-like function could contribute to IGF1-mediated, IIS-dependent effects on lifespan and immunity in A. stephensi.

Generating genetically modified fit mosquitoes that resist malaria parasite infection is a central goal of malaria transmission control research. Our efforts to genetically manipulate IIS to regulate mosquito immunity and fitness have resulted in both short- and long-lived mosquitoes that can resist P. falciparum infection, phenotypes that are defined by patterns of epithelial homeostasis and midgut integrity. The results detailed here provide evidence that IGF1 can fine-tune the balance of these processes in the mosquito midgut, effecting enhanced survival and enhanced anti-parasite resistance. Further, our studies provide critical insights into manipulation of conserved IIS regulation of mitochondrial integrity and epithelial homeostasis for concurrent optimization of fitness and anti-pathogen resistance that can be broadly applied to mosquito-focused control strategies for malaria and other vector-borne diseases.

Materials and Methods

Mosquito cell culture, mosquito rearing, and experimental treatments

Immortalized, ASE cell lines were maintained as previously described [9]. For in vivo studies, A. stephensi Liston (Indian wild-type strain) were reared and maintained at 27°C and 75% humidity. All mosquito rearing and feeding protocols were approved and in accordance with regulatory guidelines and standards set by the Institutional Animal Care and Use Committee of the University of California, Davis. For most experimental treatments, laboratory-reared 3–5 day old female mosquitoes were maintained on water for 24–48 h and then allowed to feed for 30 min on reconstituted human blood meals (healthy male adult, blood type O+) provided through a Hemotek Insect Feeding System (Discovery Workshops, Accrington, UK). Reconstituted blood meals contained washed human red blood cells (RBCs) and saline (10 mM, NaHCO₃, 15 mM NaCl, pH 7.0) as 50% RBCs and 50% saline with or without recombinant human IGF1. Human RBCs were purchased from Interstate Blood Bank (Memphis, TN, USA). Recombinant human IGF1 was purchased from R&D Systems (Minneapolis, MN, USA).

Western blotting

Female 3–5 day old mosquitoes were maintained on water for 24 h and then allowed to feed for 30 min on a reconstituted blood meal supplemented with low (0.013 µM) IGF1, high (0.13 µM) IGF1 or with phosphate-buffered saline (PBS) as a control. Protocols for analysis of protein phosphorylation have been described previously [17]. In brief, protein lysates from dissected midguts from 30 blood fed mosquitoes per treatment group were probed using primary and secondary antibodies at the following dilutions: 1∶1,250 phospho-JNK (1°; Biosource, Carlsbad, CA)/1∶20,000 rabbit anti-mouse IgG (2°; Sigma-Aldrich, St. Louis, MO) and 1∶10,000 GAPDH (1°; Abcam, San Francisco, CA)/1∶20,000 goat anti-rabbit IgG (2°; Cell Signaling Technology, Danvers, MA). Blots were developed using SuperSignal West Dura chemiluminescent reagent (Thermo Fisher Scientific, Rockford, IL), and visualized using the Kodak Image Station 4000MM Pro and Molecular Imaging software (Carestream Health, Rochester, NY). Experiments were replicated four times with separate cohorts of mosquitoes. Data were analyzed by Mann Whitney test (Graphpad Prism 6.0).

Cell culture, transfection, and luciferase reporter assays

Defensin and Gambicin promoters were cloned into the promoter-less luciferase reporter pGL3 (Promega; Madison, Wisconsin). Transfections of A. stephensi ASE cells were performed using Effectene transfection reagent (Qiagen; Valencia, CA) as previously described [14]. At 24 h post-transfection, cells were challenged with 100 µg/ml LPS (Escherichia coli serotype 026:B6; Sigma-Aldrich) with or without 0.013–1.3 µM recombinant human IGF1 (Sigma-Aldrich). Luciferase activity was measured by microplate reader (Hidex Chameleon, LabLogic; Brandon, FL) using the Dual-Glo Luciferase Assay (Promega) at 24 h post-immune challenge. Data were analyzed by unpaired t-tests comparing each treatment to matched buffer controls. Experiments were replicated three (Gambicin) or four (Defensin) times.

Preparation of P. falciparum freeze-thaw parasite product (FTPP)

P. falciparum FTPP was used to induce uniform signaling events in A. stephensi cells in vivo. Preparation and feeding of FTPP to A. stephensi were described in detail in [9], [32]. In brief, FTPP was prepared from 15 day old cultures (or when stage V gametocytes were evident) of P. falciparum-infected RBCs subjected to three freeze/thaw cycles of −80°C for 10 min followed by 37°C for 10 min. To mimic parasite infection, FTPP was diluted with uninfected human RBCs and heat-inactivated human serum (type A+, Interstate Blood Bank). As a control, an equal volume of uninfected RBCs was similarly frozen and thawed (FT-RBC) and diluted with intact uninfected RBCs and heat-inactivated human serum (type A+). All blood meals were 50% FTPP/RBCs and 50% human serum. Female mosquitoes were fed with FTPP and FT-RBC supplemented with low (0.013 µM) and high (0.13 µM) IGF1 or with PBS as a control, and processed for immune gene expression analysis by qRT-PCR as described below. Experiments were replicated six times with separate cohorts of mosquitoes. Data were analyzed using Kruskal-Wallis followed by Dunn's post-test.

Quantitative reverse-transcriptase-PCR analyses (qRT-PCR)

Female mosquitoes (3–5 d old) were maintained on water for 24 h and then allowed to feed for 30 min on a reconstituted blood meal supplemented with low (0.013 µM) IGF1, high (0.13 µM) IGF1 or PBS as a control, or FTPP/FT-RBC similarly supplemented as described above. At various times post-blood feeding, a total of 25–30 midguts were dissected into RNA-later (Life Technologies, Grand Island, NY) from each treatment group per experiment. Dissected midguts were homogenized in TRIZOL (Life Technologies) and RNA was extracted according to the manufacturer's protocol. cDNAs were synthesized from RNA samples using the SuperScript III First-Strand Synthesis System (Life Technologies). Quantitative RT-PCR was performed using Maxima SYBR green/ROX qPCR Master Mix (Thermo Fisher Scientific) on an ABI 7300 Sequence Detection System (Life Technologies). Expression levels were calculated using the 2^−ΔΔCt method relative to the ribosomal protein s7 (RPS7) gene. Relative transcript levels for RPS7, LRIM, LRRD7, TEP1, APL1, and NOS were determined using primers and cycling conditions described in [32]. For qRT-PCR assays for transcript levels for ATG6, ATG8, Pros, and Esg, cDNA samples were pre-amplified using gene specific primers prior to qPCR as described in [31]. Experiments were replicated 4–6 times with separate cohorts of mosquitoes. Normally distributed data were analyzed by unpaired t-test, and non-normally distributed data were analyzed by Mann Whitney test relative to matched controls.

ROS assays

For detection of superoxide, groups of 50 (3–5 d old) female A. stephensi mosquitoes were held on water overnight, then allowed to feed for 30 min on an ATP-saline solution (150 mM NaCl, 10 mM NaHCO₃ and 1 mM ATP) supplemented with equivalent volumes of one of the following: PBS as a control, rotenone (1 µM) to induce the formation of superoxide as a positive control, or human IGF1. Non-fed mosquitoes were removed at the end of 30 min. At 6 h post feeding, two pools of 10 midguts for each treatment were dissected and incubated in 150 µl of 5 µM MitoSOX Red (Life Technologies) in wells of a 96-well plate. The plate was incubated in the dark at room temperature for 30 min and then read at excitation 485 nm and emission 560 nm (Hidex Chameleon, LabLogic). Non-fed mosquito midguts were used to define baseline superoxide levels. Confocal images were prepared from additional midguts from the same feeding groups. For microscopy, midguts were mounted in Prolong Gold with DAPI (Life Technologies), imaged using an Olympus FV1000 confocal microscope (Olympus; Center Valley, PA) and processed using ImageJ [65] and Adobe Photoshop. Treatment data were analyzed by unpaired t-test relative to matched controls. Experiments were replicated four times with separate cohorts of mosquitoes. For detection of midgut peroxides, pools of 5 dissected midguts in 50 µL PBS (two per treatment) were homogenized by brief sonication and centrifuged to pellet debris. Peroxides were measured in 20 µL of sample supernatant in duplicate using the Pierce Quantitative Peroxide assay kit (Thermo Fisher Scientific) as per manufacturer's instructions. Samples were incubated for 20 min at room temperature prior to spectrophotometer reading. To quantify peroxide levels at 6 h and 24 h, when blood in the midgut would confound the assay, mosquitoes were fed on a ATP-saline solution (150 mM NaCl, 10 mM NaHCO₃ and 1 mM ATP) containing the following treatments: PBS control, low IGF1 (0.013 µM) or high IGF1 (0.13 µM). For each assay, three pools of five midguts per treatment were collected and processed as described above. Peroxide assays were replicated three times (blood-fed and saline-fed) with separate cohorts of mosquitoes and treatment data were analyzed by unpaired t-test relative to matched controls.

Caspase-3 activity assay

Female 3–5 day old A. stephensi were fed reconstituted human blood meals supplemented with equivalent volumes of PBS, low IGF1 (0.013 µM) or high IGF1 (0.13 µM) and midgut caspase-3 activity was measured at various times following treatment. For extended times (two and three weeks), mosquitoes were fed once per week on supplemented blood meals (PBS or IGF1) and allowed to oviposit once per week. Pools of 5 midguts per treatment were placed in 50 µL PBS; 3 pools were collected for 72 h and one week timepoints and 1–2 pools were collected for two and three week timepoints. Midguts were dissected, homogenized by brief sonication, and centrifuged to pellet debris. A total of 25 µL of supernatant was added to 25 µL PBS with 1 mM DTT, and 10 µL of the resulting solution was analyzed in duplicate using the caspase-3 fluorometric substrate DEVD-AFC (Biovision, Milpitas, CA) per manufacturer's instructions. An additional 10 µL of sample was treated with the caspase-3 inhibitor Z-DEVD-FMK (Biovision) for a negative control. Samples were incubated at 37°C in the dark and fluorescence readings were taken at 30 min and 2 h after reagents were added. Experiments were replicated 4–6 times with separate cohorts of mosquitoes. Caspase-3 activity among groups was compared using Kolmogorov-Smirnov test.

Functional assay of midgut permeability in IGF1-fed A. stephensi

Female 3–5 day old A. stephensi were kept on water for 48 h and then allowed to feed for 30 min on reconstituted human blood meals supplemented with equivalent volumes of low (0.013 µM) IGF1, high (0.13 µM) IGF1 or PBS as a control together with 1×10⁶ fluorescent beads/ml (3.0–3.4 µm, Sphero Rainbow Calibration particles RCP-30-5A-2; Spherotech, Lake Forest, IL) as described [31]. Non-fed mosquitoes were removed immediately after feeding. At 72 h post-feeding, samples of three whole mosquitoes or three dissected midguts were placed in PBS, pulse sonicated, and filtered through a 35 µm nylon mesh to remove tissue debris. Sample data were acquired with a FACScan flow cytometer (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (version 6.4.1; Tree Star). To remove the contribution of beads remaining in the midgut from the whole body bead count the beads in three midguts (pooled) were quantified and subtracted from each analyzed sample of three whole mosquitoes (pooled). Five replicates of three whole pooled mosquitoes were analyzed per experiment. Experiments were replicated with two separate cohorts of mosquitoes. Statistical significance was determined by one-way ANOVA followed by Fisher's LSD.

Malaria parasite infection/L-NAME treatment

P. falciparum NF54 strain was cultured in 10% heat-inactivated human serum and 6% washed human RBCs in RPMI 1640 with HEPES (Life Technologies) and hypoxanthine for 15 days or until stage V gametocytes were evident. Exflagellation rates of mature gametocytes were evaluated on the day prior to and the day of mosquito infection. Mosquitoes were fed on mature gametocyte cultures diluted with human RBCs and heat-inactivated human serum (final concentration 50% RBCs, 50% serum) for 30 min. Protocols for the culture and handling of P. falciparum for mosquito feeding were approved and in accordance with regulatory guidelines and standards set by the Biological Safety Administrative Advisory Committee of the University of California, Davis.

For NOS inhibition, 125 female A. stephensi were provided with water only, with 3.7 µM L-NAME (Sigma-Aldrich), or with 0.13 µM IGF1 plus L-NAME from 72 h before blood feeding, at the time of P. falciparum infection (supplemented into the blood meal), and thereafter until dissection. After 10 days, fully gravid females were dissected and midguts were stained with 0.1% mercurochrome to visualize P. falciparum oocysts. Oocysts were counted for each midgut and mean oocysts per midgut (infection intensity) and percentages of infected mosquitoes (infection prevalence; infection defined as at least one oocyst) were calculated for all dissected mosquitoes. This experiment was repeated four times with separate cohorts of mosquitoes. Infection intensity data were analyzed by Kruskal-Wallis test to determine whether the oocysts per midgut in the controls differed among replicates. Since no differences were evident, the data were pooled across replicates. Oocyst counts were compared using the Mann-Whitney test for nonparametric data. Infection prevalence data were analyzed by Fisher's exact test to determine whether infection status differed between treatment conditions.

Oxidative stress survivorship assays

100 female 3–5 day old A. stephensi were kept on water for 48 h, and then fed for 30 min on a saline/ATP solution (150 mM NaCl, 10 mM NaHCO₃, 1 mM ATP) supplemented with 1 mM paraquat (Sigma-Aldrich) and equivalent volumes of PBS, low IGF1 (0.013 µM) or high IGF1 (0.13 µM). Dead mosquitoes were removed and counted at various times between 8 h and 72 h. The experiment was replicated twice with separate cohorts of mosquitoes. Survival analyses were performed using the Kaplan Meier method and differences between survival curves calculated using the Wilcoxon test.

Supporting Information

Figure S1

Raw (non-normalized) data from Figure 2A . Human IGF1 did not alter antimicrobial peptide (AMP) promoter activity in immune-activated ASE cells. Graphs depicts relative light units (RLU) from promoter-reporter assays in transfected cells stimulated with or without LPS and with or without IGF1.

(TIF)

Figure S2

Raw (non-normalized) data from Figure 6 . Peroxide levels in midguts at 72 h (A) and one week (C) following IGF1 treatment. Peroxides were quantified in pools of five midguts; three pools were collected per treatment per experiment. Experiments were replicated three times with separate cohorts of mosquitoes. Graphs show the µM H₂O₂/midgut. Midgut caspase-3 activity at 72 h (B), one week (D), two weeks (E), and three weeks (F) following IGF1 treatment. Graphs show AFC fluorescence (caspase-3 activity). Three pools of five midguts per treatment were collected for 72 h and one week timepoints and 1–2 pools were collected for two and three week timepoints. Experiments were replicated 4–6 times with separate cohorts of mosquitoes.

(TIF)

Funding Statement

This work was funded by support from the National Institutes of Health (AI073745 to MAR, SL; AI080799 to SL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.