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. 1995 Oct 24;92(22):10104–10108. doi: 10.1073/pnas.92.22.10104

A detergent-free method for purifying caveolae membrane from tissue culture cells.

E J Smart 1, Y S Ying 1, C Mineo 1, R G Anderson 1
PMCID: PMC40744  PMID: 7479734

Abstract

Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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