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. Author manuscript; available in PMC: 2014 Jun 28.
Published in final edited form as: Biochemistry. 2010 Jun 22;49(24):4977–4986. doi: 10.1021/bi1001739

Figure 3.

Figure 3

A. The effects of mutations that reduce steric hindrance by His64 on bound CNC4. In P6 crystals at pH 9.0, the bound ligand changed from the in conformation for wt MbCNC4 to the out conformation for both the H64A (bottom left) and F46V (bottom right) MbCNC4 complexes. B. The effects of the same mutations on the νCN bands in FTIR spectra. The IR spectra were collected at pH 7.0 in 0.1 M phosphate buffer.