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. 1995 Dec;48(6):M342–M346. doi: 10.1136/mp.48.6.m342

Circulating IgG autoanti-IgE antibodies in atopic patients block the binding of IgE to its low affinity receptor (CD23)

S J Smith 1,2, N S Jones 1,2, F Shakib 1,2
PMCID: PMC408002  PMID: 16696035

Abstract

Aims—To investigate the ability of circulating IgG autoanti-IgE antibodies from atopic rhinitis patients to block the binding of IgE to its low affinity receptor (FcεRII), also termed CD23.

Methods—This involved the use of a well validated flow cytometric method to detect inhibition of FITC labelled IgE binding to human B cells expressing CD23 (RPMI 8866 cell line).

Results—Taking inhibition values greater than 20% as being significant, 15 out of 20 IgG anti-IgE containing sera inhibited the binding of IgE-FITC to the RPMI 8866 cells. The inhibitory effect was recoverable in the IgG fraction of serum, but was not related to the titre of either IgG1 anti-IgE or IgG4 anti-IgE, thus suggesting that it might be related to epitope specificity. No such inhibition was demonstrable with rheumatoid sera containing autoanti-IgG (that is, rheumatoid factor), but lacking autoanti-IgE.

Conclusions—The capacity of anti-IgE to block the binding of IgE to CD23 has important implications, particularly in terms of upregulation of IgE synthesis and the consequent perpetuation of the inflammatory response.

Keywords: Atopy

Keywords: autoanti-IgE

Keywords: CD23

Keywords: IgE

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M342

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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