Skip to main content
NCBI home page
Clinical Molecular Pathology logoLink to Clinical Molecular Pathology
. 1996 Feb;49(1):M43–M45. doi: 10.1136/mp.49.1.m43

Accumulation of p53 protein in inverted transitional cell papilloma of the urinary bladder

K W Chan 1, K Y Lam 1, G Srivastava 1
PMCID: PMC408017  PMID: 16696044

Abstract

Aims—To study the possible accumulation of p53 protein in inverted papilloma of the urinary bladder.

Methods—Formalin fixed, paraffin wax embedded sections from 14 cases of inverted papilloma of the urinary bladder were studied retrospectively. Accumulation of p53 was detected by immunohistochemistry using a mouse monoclonal antibody directed against p53. p53 protein reactivity was scored as follows: 0 = 10%; 1 = 10% to <30%; 2 = 30% to <50%; and 3 = >50% of cells p53 positive.

Results—The 14 sections were scored as follows: 3 in four cases; 2 in four cases; 1 in one case; and 0 in five cases. Overall, nine (64%) of the 14 cases were positive for p53 protein.

Conclusions—The accumulation of p53 protein in inverted papilloma of the urinary bladder suggests that p53 may have has an important role in the neoplastic process of this tumour. However, the benign nature of inverted papillomas suggests that p53 protein accumulation is not related to tumour invasiveness and metastasis. p53 reactivity cannot be used as a marker of malignancy for urothelial neoplasia. Further studies are required to determine the role of p53 protein in the oncogenesis of urothelial neoplasms.

Keywords: immunohistochemistry

Keywords: p53

Keywords: inverted papilloma

Keywords: urothelial neoplasm

Full text

PDF
M43

Images in this article

M44Image
on p.M44

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes D. M., Hanby A. M., Gillett C. E., Mohammed S., Hodgson S., Bobrow L. G., Leigh I. M., Purkis T., MacGeoch C., Spurr N. K. Abnormal expression of wild type p53 protein in normal cells of a cancer family patient. Lancet. 1992 Aug 1;340(8814):259–263. doi: 10.1016/0140-6736(92)92354-i. [DOI] [PubMed] [Google Scholar]
  2. Esrig D., Elmajian D., Groshen S., Freeman J. A., Stein J. P., Chen S. C., Nichols P. W., Skinner D. G., Jones P. A., Cote R. J. Accumulation of nuclear p53 and tumor progression in bladder cancer. N Engl J Med. 1994 Nov 10;331(19):1259–1264. doi: 10.1056/NEJM199411103311903. [DOI] [PubMed] [Google Scholar]
  3. Esrig D., Spruck C. H., 3rd, Nichols P. W., Chaiwun B., Steven K., Groshen S., Chen S. C., Skinner D. G., Jones P. A., Cote R. J. p53 nuclear protein accumulation correlates with mutations in the p53 gene, tumor grade, and stage in bladder cancer. Am J Pathol. 1993 Nov;143(5):1389–1397. [PMC free article] [PubMed] [Google Scholar]
  4. Grainger R., Gikas P. W., Grossman H. B. Urothelial carcinoma occurring within an inverted papilloma of the ureter. J Urol. 1990 Apr;143(4):802–804. doi: 10.1016/s0022-5347(17)40101-7. [DOI] [PubMed] [Google Scholar]
  5. Hall P. A., McKee P. H., Menage H. D., Dover R., Lane D. P. High levels of p53 protein in UV-irradiated normal human skin. Oncogene. 1993 Jan;8(1):203–207. [PubMed] [Google Scholar]
  6. Kunimi K., Uchibayashi T., Egawa M. A case of inverted papilloma of the ureter: is the DNA ploidy pattern associated with occurrence of transitional cell carcinoma of the bladder? Int Urol Nephrol. 1994;26(1):17–22. doi: 10.1007/BF02768239. [DOI] [PubMed] [Google Scholar]
  7. Lane D. P., Benchimol S. p53: oncogene or anti-oncogene? Genes Dev. 1990 Jan;4(1):1–8. doi: 10.1101/gad.4.1.1. [DOI] [PubMed] [Google Scholar]
  8. Mellon K., Wilkinson S., Vickers J., Robinson M. C., Shenton B. K., Neal D. E. Abnormalities in p53 and DNA content in transitional cell carcinoma of the bladder. Br J Urol. 1994 May;73(5):522–525. doi: 10.1111/j.1464-410x.1994.tb07637.x. [DOI] [PubMed] [Google Scholar]
  9. Moch H., Sauter G., Moore D., Mihatsch M. J., Gudat F., Waldman F. p53 and erbB-2 protein overexpression are associated with early invasion and metastasis in bladder cancer. Virchows Arch A Pathol Anat Histopathol. 1993;423(5):329–334. doi: 10.1007/BF01607144. [DOI] [PubMed] [Google Scholar]
  10. Mulkens T. H., Vereycken H. A., Van Nueten J. C., Govaerts G. C., De Schepper A. M., Van Camp K. O., Van Marck E. A. Bilateral ureteral inverted papillomas in association with bladder carcinoma: a case report and review of the literature. Urol Radiol. 1990;12(3):154–156. doi: 10.1007/BF02923996. [DOI] [PubMed] [Google Scholar]
  11. Pignatelli M., Stamp G. W., Kafiri G., Lane D., Bodmer W. F. Over-expression of p53 nuclear oncoprotein in colorectal adenomas. Int J Cancer. 1992 Mar 12;50(5):683–688. doi: 10.1002/ijc.2910500503. [DOI] [PubMed] [Google Scholar]
  12. Sarkis A. S., Dalbagni G., Cordon-Cardo C., Melamed J., Zhang Z. F., Sheinfeld J., Fair W. R., Herr H. W., Reuter V. E. Association of P53 nuclear overexpression and tumor progression in carcinoma in situ of the bladder. J Urol. 1994 Aug;152(2 Pt 1):388–392. doi: 10.1016/s0022-5347(17)32745-3. [DOI] [PubMed] [Google Scholar]
  13. Schmitz-Dräger B. J., van Roeyen C. R., Grimm M. O., Gerharz C. D., Decken K., Schulz W. A., Bültel H., Makri D., Ebert T., Ackermann R. P53 accumulation in precursor lesions and early stages of bladder cancer. World J Urol. 1994;12(2):79–83. doi: 10.1007/BF00184241. [DOI] [PubMed] [Google Scholar]
  14. Talbert M. L., Young R. H. Carcinomas of the urinary bladder with deceptively benign-appearing foci. A report of three cases. Am J Surg Pathol. 1989 May;13(5):374–381. doi: 10.1097/00000478-198905000-00004. [DOI] [PubMed] [Google Scholar]
  15. Tenaud C., Negoescu A., Labat-Moleur F., Legros Y., Soussi T., Brambilla E. p53 immunolabeling in archival paraffin-embedded tissues: optimal protocol based on microwave heating for eight antibodies on lung carcinomas. Mod Pathol. 1994 Oct;7(8):853–859. [PubMed] [Google Scholar]

Articles from Clinical Molecular Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES