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. 1996 Jun;49(3):M166–M169. doi: 10.1136/mp.49.3.m166

Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene

J Polanec 1,2, Z P Pavelic 1,2, W L Myers 1,2
PMCID: PMC408044  PMID: 16696066

Abstract

Aims—To investigate the effect of Wnt-1 antisense RNA on the outgrowth of a mammary tumour cell line expressing that oncogene.

Methods—A plasmid (pMT 70), containing Wnt-1 cDNA, was cut with appropriate enzymes and inserted into a eukaryotic expression vector (pMAMneo). A mammary tumour cell line (CAC-L153) was transfected with the expression vector and cells with the vector in sense and antisense orientation were selected.

Results—Tumour cells with the expression vector in the antisense orientation had a notable reduction in expression of Wnt-1 protein and a considerable reduction in tumour outgrowth compared with controls.

Conclusions—The results indicate that the Wnt-1 proto-oncogene may be a possible target for antisense therapy.

Keywords: mammary adenocarcinoma

Keywords: cell lines

Keywords: Wnt-1 oncogene

Keywords: RNA

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Selected References

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