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Study of inhibitory effects of CCCP (0.1 mM) on efflux function of MexAB-OprM and MexA-(EGFP-MexB)-OprM transporters in live cells. Time-dependent fluorescence intensity of EtBr (15 μM) incubated with the cells (OD600 nm = 0.1 in PBS buffer, pH 7.2): (A) WT; (B) MexA-(EGFP-MexB)-OprM; (C) ΔMexB; (D) ΔABM; and (E) nalB1 strains, in (a) the presence and (b) absence of CCCP.
