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. 1995 Oct 10;92(21):9465–9469. doi: 10.1073/pnas.92.21.9465

A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defense.

B Lemaitre 1, E Kromer-Metzger 1, L Michaut 1, E Nicolas 1, M Meister 1, P Georgel 1, J M Reichhart 1, J A Hoffmann 1
PMCID: PMC40822  PMID: 7568155

Abstract

In this paper we report a recessive mutation, immune deficiency (imd), that impairs the inducibility of all genes encoding antibacterial peptides during the immune response of Drosophila. When challenged with bacteria, flies carrying this mutation show a lower survival rate than wild-type flies. We also report that, in contrast to the antibacterial peptides, the antifungal peptide drosomycin remains inducible in a homozygous imd mutant background. These results point to the existence of two different pathways leading to the expression of two types of target genes, encoding either the antibacterial peptides or the antifungal peptide drosomycin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bulet P., Dimarcq J. L., Hetru C., Lagueux M., Charlet M., Hegy G., Van Dorsselaer A., Hoffmann J. A. A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution. J Biol Chem. 1993 Jul 15;268(20):14893–14897. [PubMed] [Google Scholar]
  2. Dimarcq J. L., Hoffmann D., Meister M., Bulet P., Lanot R., Reichhart J. M., Hoffmann J. A. Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. A study in insect immunity. Eur J Biochem. 1994 Apr 1;221(1):201–209. doi: 10.1111/j.1432-1033.1994.tb18730.x. [DOI] [PubMed] [Google Scholar]
  3. Engström Y., Kadalayil L., Sun S. C., Samakovlis C., Hultmark D., Faye I. kappa B-like motifs regulate the induction of immune genes in Drosophila. J Mol Biol. 1993 Jul 20;232(2):327–333. doi: 10.1006/jmbi.1993.1392. [DOI] [PubMed] [Google Scholar]
  4. Fehlbaum P., Bulet P., Michaut L., Lagueux M., Broekaert W. F., Hetru C., Hoffmann J. A. Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides. J Biol Chem. 1994 Dec 30;269(52):33159–33163. [PubMed] [Google Scholar]
  5. Georgel P., Meister M., Kappler C., Lemaitre B., Reichhart J. M., Hoffmann J. A. Insect immunity: the diptericin promoter contains multiple functional regulatory sequences homologous to mammalian acute-phase response elements. Biochem Biophys Res Commun. 1993 Dec 15;197(2):508–517. doi: 10.1006/bbrc.1993.2508. [DOI] [PubMed] [Google Scholar]
  6. Hoffmann J. A. Innate immunity of insects. Curr Opin Immunol. 1995 Feb;7(1):4–10. doi: 10.1016/0952-7915(95)80022-0. [DOI] [PubMed] [Google Scholar]
  7. Hultmark D. Immune reactions in Drosophila and other insects: a model for innate immunity. Trends Genet. 1993 May;9(5):178–183. doi: 10.1016/0168-9525(93)90165-e. [DOI] [PubMed] [Google Scholar]
  8. Ip Y. T., Levine M. Molecular genetics of Drosophila immunity. Curr Opin Genet Dev. 1994 Oct;4(5):672–677. doi: 10.1016/0959-437x(94)90133-n. [DOI] [PubMed] [Google Scholar]
  9. Ip Y. T., Reach M., Engstrom Y., Kadalayil L., Cai H., González-Crespo S., Tatei K., Levine M. Dif, a dorsal-related gene that mediates an immune response in Drosophila. Cell. 1993 Nov 19;75(4):753–763. doi: 10.1016/0092-8674(93)90495-c. [DOI] [PubMed] [Google Scholar]
  10. Kappler C., Meister M., Lagueux M., Gateff E., Hoffmann J. A., Reichhart J. M. Insect immunity. Two 17 bp repeats nesting a kappa B-related sequence confer inducibility to the diptericin gene and bind a polypeptide in bacteria-challenged Drosophila. EMBO J. 1993 Apr;12(4):1561–1568. doi: 10.1002/j.1460-2075.1993.tb05800.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kylsten P., Samakovlis C., Hultmark D. The cecropin locus in Drosophila; a compact gene cluster involved in the response to infection. EMBO J. 1990 Jan;9(1):217–224. doi: 10.1002/j.1460-2075.1990.tb08098.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lemaitre B., Coen D. P regulatory products repress in vivo the P promoter activity in P-lacZ fusion genes. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4419–4423. doi: 10.1073/pnas.88.10.4419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lemaitre B., Meister M., Govind S., Georgel P., Steward R., Reichhart J. M., Hoffmann J. A. Functional analysis and regulation of nuclear import of dorsal during the immune response in Drosophila. EMBO J. 1995 Feb 1;14(3):536–545. doi: 10.1002/j.1460-2075.1995.tb07029.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meister M., Braun A., Kappler C., Reichhart J. M., Hoffmann J. A. Insect immunity. A transgenic analysis in Drosophila defines several functional domains in the diptericin promoter. EMBO J. 1994 Dec 15;13(24):5958–5966. doi: 10.1002/j.1460-2075.1994.tb06941.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Connell P. O., Rosbash M. Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene. Nucleic Acids Res. 1984 Jul 11;12(13):5495–5513. doi: 10.1093/nar/12.13.5495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reichhart J. M., Georgel P., Meister M., Lemaitre B., Kappler C., Hoffmann J. A. Expression and nuclear translocation of the rel/NF-kappa B-related morphogen dorsal during the immune response of Drosophila. C R Acad Sci III. 1993 Oct;316(10):1218–1224. [PubMed] [Google Scholar]
  17. Reichhart J. M., Meister M., Dimarcq J. L., Zachary D., Hoffmann D., Ruiz C., Richards G., Hoffmann J. A. Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter. EMBO J. 1992 Apr;11(4):1469–1477. doi: 10.1002/j.1460-2075.1992.tb05191.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rubin G. M., Spradling A. C. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 1983 Sep 24;11(18):6341–6351. doi: 10.1093/nar/11.18.6341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tryselius Y., Samakovlis C., Kimbrell D. A., Hultmark D. CecC, a cecropin gene expressed during metamorphosis in Drosophila pupae. Eur J Biochem. 1992 Feb 15;204(1):395–399. doi: 10.1111/j.1432-1033.1992.tb16648.x. [DOI] [PubMed] [Google Scholar]
  20. Wicker C., Reichhart J. M., Hoffmann D., Hultmark D., Samakovlis C., Hoffmann J. A. Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides. J Biol Chem. 1990 Dec 25;265(36):22493–22498. [PubMed] [Google Scholar]

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