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. 2014 Apr 2;3(3):288–298. doi: 10.1002/mbo3.167

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© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Chronological life span and petite formation. Panel A: comparison of chronological viability of WT strains grown in YP liquid medium containing 2% glucose, reported as percentages of colony forming units. Stationary phases were taken as 100%. Panel B: comparison of petite colonies formation during chronological survival as in panel A. For each analysis (A, B) the same aliquots of cultures were diluted and plated on solid medium. Large and petite colonies forming units were counted after 3 days. Panel C: comparison of chronological viability of rho° strains performed as in panel A. Data are means of at least three independent experiments, with reported standard deviations bars.