Figure 1. Effects of Toxoplasma infection on the expression of miR-132 and its target genes determined by qPCR.
(A) MiR-132 is highly expressed in human neuroepithelioma cells 20 hours following infection with 3 canonical Toxoplasma strains. (B) Four putative miR-132 targets were downregulated in infected cells compared to controls, regardless of the parasite genotype. The fold expression of miR-132 was normalized by an endogenous control (RNU48), while the putative targets were normalized by β-actin. Each strain n = 3; error bars = 1 SD. *P < 0.05, **P < 0.01.