Abstract
Background:
Aberrant activation of Wnt/β-catenin has been implicated in various cancer-related processes, for example, proliferation or tumour cell survival. However, the exact mechanism by which β-catenin provides liver tumour cells with a selective advantage is still unclear. This study was aimed to analyse growth behaviour and survival of β-catenin-driven mouse liver tumours after β-catenin ablation.
Methods:
Transgenic mice with a controllable hepatocyte-specific knockout of Ctnnb1 (encoding β-catenin) were generated and liver tumours were induced by means of a N-nitrosodiethylamine/phenobarbital tumour initiation/promotion protocol, which leads to the outgrowth of hepatocellular tumours with activated β-catenin. Cre recombinase was activated and the effects of the knockout in the tumours were studied.
Results:
Activation of Cre recombinase led to the knockout of Ctnnb1 in a fraction of tumour cells, thus resulting in the formation of two different tumour cell subpopulations, with or without β-catenin. Comparative analysis of the two subpopulations revealed that cell proliferation was significantly decreased in Ctnnb1-deleted hepatoma cells, compared with the corresponding non-deleted cell population, whereas no increased rate of apoptosis after knockout of Ctnnb1 was observed.
Conclusions:
β-catenin-dependent signalling is an important regulator of hepatoma cell growth in mice, but not a crucial factor in the regulation of tumour survival.
Keywords: Wnt/β-catenin signalling, liver, hepatocellular tumour, Connexin 32, proliferation, Ctnnb1, human hepatocellular carcinomas
Up to 30% of human hepatocellular carcinomas and 80% of hepatoblastomas display aberrant activation of the Wnt/β-catenin signalling pathway (de La Coste et al, 1998; Lopez-Terrada et al, 2009; Schmidt et al, 2011). This is mainly caused by genetic alterations in the CTNNB1 gene, encoding β-catenin, an important transcription factor in canonical Wnt signalling. Mutations in CTNNB1 lead to amino acid exchanges near the N terminus of the β-catenin protein where important phosphorylation sites are located, which are essential for its proteasomal degradation. On accumulation in the cytoplasm, mutant β-catenin translocates into the nucleus to associate with T-cell factor/lymphoid enhancer factor proteins and to promote the expression of target genes (Lustig and Behrens, 2003).
In mouse liver, tumours harbouring a mutationally activated Wnt/β-catenin signalling pathway can be induced by an initiation/promotion regimen according to Moennikes et al (2000): 6-week-old mice are given a single dose of the liver carcinogen N-nitrosodiethylamine (DEN) followed by chronic treatment with phenobarbital (PB). Using this regimen, the tumour promoter PB selects for the outgrowth of Ctnnb1-mutated hepatoma cells (Ctnnb1 is the mouse orthologue to CTNNB1 in humans) and the frequency of liver tumours mutated in Ctnnb1 is about 80% (Aydinlik et al, 2001).
There is evidence that aberrant β-catenin-dependent signalling promotes cell proliferation and inhibits apoptosis, thus providing cancer cells with a selective advantage. Expression of an oncogenic form of β-catenin leads to hepatomegaly in transgenic mice due to increased hepatocyte proliferation (Cadoret et al, 2001). Furthermore, several known target genes of β-catenin are implicated in cell cycle progression, such as cyclin D1 and c-myc (He et al, 1998; Shtutman et al, 1999), or in the regulation of apoptosis (Zhang et al, 2001). Activation of β-catenin in different cell lines results in increased proliferation, while at the same time inhibition of apoptosis is observed (Orford et al, 1999; Shang et al, 2004). Aberrant Wnt signalling is sufficient to generate intestinal lesions and adenomas (Shibata et al, 1997; Romagnolo et al, 1999) and is also required for maintenance of colorectal tumour xenografts in mice (Scholer-Dahirel et al, 2011). In liver, β-catenin-positive cell islets, which have evaded the hepatocyte-specific knockout of Ctnnb1 in respective transgenic mice, show a higher proliferative index in presence of PB and in livers with a pre-cirrhotic phenotype, as compared with surrounding hepatocytes lacking β-catenin (Braeuning et al, 2010). This suggests that, at least under certain conditions, β-catenin provides a growth advantage to hepatocytes, thereby contributing to liver carcinogenesis. Respective cell islets also exhibit reduced levels of the gap junction-forming protein connexin 32 (Cx32), similar to what had been observed earlier in Ctnnb1-mutated liver tumours (Moennikes et al, 2000; Marx-Stoelting et al, 2008) Thus, inhibition of cell–cell communication might be involved in the regulation of hepatoma cell proliferation by β-catenin.
In a study conducted by Malanchi et al (2008), transgenic mice (referred to as K14-creERT2:β-cateninlox/lox mice) were used to investigate the role of β-catenin in established chemically induced skin tumours with increased Wnt/β-catenin signalling. Expression of Cre recombinase under the control of the keratin 14 (K14) locus resulted in the deletion of Ctnnb1 in epidermal cells and subsequent complete tumour regression within 5–6 weeks, clearly indicating the important role of the signalling protein for tumour cell survival in this model.
Despite the impressive results from the above-mentioned experiment, the relevance of β-catenin activation for proliferation and survival of tumour cells in liver is not well understood. We have therefore now performed an experiment similar to that of Malanchi et al (2008), using a genetically modified mouse line that enabled us to study the effect of a tamoxifen-induced, hepatocyte-specific knockout of Ctnnb1 during chemical hepatocarcinogenesis. The results of our study show that Ctnnb1 ablation negatively affects liver tumour cell proliferation but has no significant influence on their survival.
Materials and methods
Animal breeding
Transgenic Ctnnb1loxP/loxP mice carrying loxP sites flanking the exons 3 and 6 in the Ctnnb1 gene (Huelsken et al, 2001) were interbred with TTR-Cre-Tam mice expressing a tamoxifen-inducible modified Cre recombinase (MerCreMer) under the control of the hepatocyte-specific transthyretin promoter (Tannour-Louet et al, 2002; Anson et al, 2012) to obtain Ctnnb1loxP/loxP, TTR-Cre-Tam mice (Figure 1A). Genotyping of mice for Ctnnb1loxP/loxP and Cre was performed by standard PCR as recently described (Braeuning et al, 2009). Mice carrying Ctnnb1loxP/loxP and one Cre allele are referred to as ‘Ctnnb1 KO mice' in the following text, the respective Cre-null mice are called ‘Ctnnb1 WT mice', as they are phenotypically normal. Mice were kept on a 12-h dark/light cycle and had access to food and tap water ad libitum. Animals received humane care and protocols complied with institutional guidelines.
Animal experiment
For the induction of liver tumours (Figure 1B), we followed a standard initiation/promotion protocol (Moennikes et al, 2000). Six-week-old male C3H Ctnnb1 WT (n=16) and KO (n=35) mice were given a single intraperitoneal injection of DEN (90 μg g−1 body weight; dissolved at 9 mg ml−1 in 0.9% NaCl solution, injected volume 10 μl g−1 body weight). After a treatment-free interval of 3 weeks, mice were kept on a diet containing 0.05% PB (Ssniff, Soest, Germany) for a time period of 25 weeks. After 1 week on a PB-free control diet, mice were then given five intraperitoneal injections of each 1.5 mg tamoxifen for 5 consecutive days. Tamoxifen (Sigma, Taufkirchen, Germany) was dissolved in ethanol (67 mg ml−1) and further diluted in corn oil to a final concentration of 10 mg ml−1 as previously described (Ganzenberg et al, 2013). Mice were killed 1–7 weeks after the last tamoxifen treatment. 5-bromodeoxyuridine (BrdU; AppliChem, Darmstadt, Germany) was dissolved in the drinking water (1 mg ml−1) and given orally to the animals for 72 h before killing. Killing was always between 0900 and 1100 h to avoid circadian influences. Livers were excised and the number and size of macroscopically visible tumours were recorded. Liver aliquots were frozen on dry ice and stored at −70 °C for further analyses or were fixed in 4% paraformaldehyde and embedded in paraffin for the preparation of TUNEL (TdT-mediated dUTP nick end labelling) stainings (see section below).
Immunohistochemical staining
Slices from frozen livers (10 μm thickness) were prepared and immunohistochemically stained as previously described (Braeuning et al, 2010), using antibodies against glutamine synthetase (GS; 1 : 1000, Sigma), β-catenin (1 : 50, Cell Signaling, Danvers, MA, USA) or BrdU (1 : 50, Dako, Glostrup, Denmark) in combination with horseradish peroxidase-conjugated secondary antibodies directed against rabbit (1 : 100, Dako) or mouse (1 : 20, Sigma) immunoglobulins with 3-amino-9 ethylcarbazole/H2O2 as substrates. For double staining of GS and Cx32, GS was visualised by the use of β-galactosidase-conjugated secondary antibodies (1 : 50, American Qualex, San Clemente, CA, USA) in combination with an antibody against Cx32 (1 : 250, Invitrogen/Zymed, Darmstadt, Germany) and horseradish peroxidase-conjugated secondary antibodies directed against rabbit (1 : 100, Dako) immunoglobulins with 3-amino-9-ethylcarbazole/H2O2 as substrates.
Tumour analyses
An Axio Imager light microscope (Imager.M1, Zeiss, Göttingen, Germany) with Axiovision Rel. 4.5 software (Zeiss) was used for acquisition of photographs and for further analysis of tumours. Counting of GS- and BrdU-positive cells within tumour subpopulations, grading of Cx32 levels and determination of tumour area fractions, which are equivalent to the tumour volume fractions, were performed using images of stained liver sections.
PCR analyses
Liver slices were stained for GS and respective tumour areas were punched out using a sharpened cannula. The tissue samples were digested with proteinase K and DNA was amplified by standard PCR methods. Tamoxifen treatment-induced Cre-mediated recombination was verified by screening for deletion of the loxP-flanked parts within the Ctnnb1 gene of four representative tumours (Figure 1C; for further details see Huelsken et al (2001)). Hot spot mutations in exon 3 of the Ctnnb1 gene in GS-positive tumours were detected by standard sequencing (Braeuning et al, 2010).
TUNEL assay
Apoptotic cell death was determined by TUNEL staining using the In Situ Cell Death Detection Kit, POD (Roche, Mannheim, Germany) according to the manufacturer's instructions for paraffin-embedded tissue sections. To induce DNA strand breaks in positive controls, sections were incubated with benzonase nuclease (Sigma) before labelling procedures.
Statistical analyses
The percentages of BrdU-labelled tumour cells were determined for the GS-negative and -positive tumour cell subpopulations within each tumour and the paired Student's T-test was used for statistical analyses. Differences were considered significant when P<0.05. Statistical significance is indicated by asterisks (*P<0.05; ***P<0.001).
Results
Tamoxifen-induced recombination of Ctnnb1 in transgenic mice
Following the induction of Cre recombinase by tamoxifen according to Ganzenberg et al (2013), PCR analyses of tumour tissue samples demonstrated Ctnnb1 deletion exclusively in the Cre-positive mice. Accordingly, the levels of non-recombined floxed Ctnnb1 were reduced in these mice (Figure 1C). Residual floxed Ctnnb1 in tumour cells from KO animals may derive from the non-parenchymal cells not expressing Cre, or from incomplete recombination in the hepatoma cells. Hepatic tumour burden (measured as the tumour volume fraction) at the time point of tamoxifen injection was ∼3%, as can be estimated from the observed tumour volume determined 1 week later at the time of killing of the first study group (compare Figure 2B).
Tumour phenotype after tamoxifen-induced KO of Ctnnb1
Morphologically, the majority (∼90% in number and size) of liver tumours were eosinophilic, well-differentiated hepatocellular adenoma. Expression of the direct β-catenin target GS is one of the most reliable markers for β-catenin activation in murine liver. It is homogeneously expressed in chemically induced mouse liver tumours and there is an almost 100% concordance between activated β-catenin and GS expression (Loeppen et al, 2002; Chafey et al, 2009). Furthermore, GS is absent from Ctnnb1 KO hepatocytes (Braeuning et al, 2010). To confirm the correlation between high GS expression and activating mutations in the Ctnnb1 gene, tumour mutation analyses were performed. Twelve out of 13 analysed GS-positive tumours (92.3%) from Ctnnb1 WT (5 out of 5 tumours Ctnnb1 mutated) or KO (7 out of 8) animals were point mutated in the hot spot regions of the Ctnnb1 gene.
Immunostains of tumours from tamoxifen-treated Ctnnb1 WT mice revealed homogeneous GS expression throughout the tumours (Figure 1D, left image), indicative of active β-catenin-dependent signalling. Accordingly, high levels of the β-catenin protein were present in the respective GS-positive tumour cells (Figure 1E). In contrast, tumours from KO animals were composed of a mixture of GS-negative and GS-positive tumour cell subpopulations (Figure 1D, images 2–4). Of note, immunostaining for β-catenin was mostly membranous, as can be expected from chemically induced mouse liver tumours that contain rather low levels of nuclear β-catenin (Devereux et al, 1999; Aydinlik et al, 2001; Braeuning et al, 2007). The proportion of GS-negative and -positive cells was at great variance between individual tumours, and no clear-cut correlation between the time point of killing and the proportion of GS-negative hepatocytes was apparent. A comparable scattered pattern of expression was seen in tumours stained for β-catenin (Figure 1E). Obviously, the recombination of floxed Ctnnb1 alleles by Cre was incomplete, leading to a situation where one fraction of tumour cells is Ctnnb1 KO and therefore GS negative, whereas the other fraction of cells still possesses a non-recombined, mutationally activated Ctnnb1 allele that drives the expression of the marker protein GS. This heterogeneous phenotype allowed us to investigate two corresponding cell populations within one and the same tumour.
Effect of β-catenin ablation on tumour cell proliferation and apoptosis
For the analysis of tumour cell proliferation, the tumours were first categorised into two groups based on the efficiency of the β-catenin KO, evidenced by loss of the marker protein GS (25–50% or >50% β-catenin-/GS-negative cells, respectively). Tumour by tumour, the fractions of BrdU-incorporating cells were determined separately for both the GS-positive and the GS-negative hepatoma cell subpopulations. Very small tumours and tumours with a poor recombination rate (that is, <25%) were omitted from the analysis, as a too low number of cells in either of the two subpopulations would lead to inconclusive results. The results of this analysis clearly demonstrated that tumour cells negative for GS exhibit a lower proliferative index as compared with their GS-positive counterparts (Figure 2A). For comparison, GS-positive cells from Ctnnb1 WT tumours had a mean BrdU-labelling index of 10.3% (data not shown), significantly higher (P<0.05; Student's t-test) than the values obtained with the GS-positive sub-populations from partially Ctnnb1-ablated tumours (4.0% for 25–50% Ctnnb1 KO; 2.2% for >50% Ctnnb1 KO). BrdU labelling in surrounding normal tissue was always <1%, irrespective of the genotype. In addition, the tumour volume fraction of Ctnnb1 WT and KO mice 1 and 7 weeks after tamoxifen treatment was determined (Figure 2B). After 7 weeks, tumour burden in livers from Ctnnb1 WT mice was significantly increased, as compared with the tumour burden 6 weeks earlier. In contrast, Ctnnb1 KO mice showed no obvious alteration in tumour volume fraction over time.
Furthermore, the impact of Ctnnb1 KO on tumour cell death was investigated 7 weeks after β-catenin ablation. At this time point, the tumour tissue showed intact hepatoma cell nuclei with no obvious signs of necrosis or inflammation. Apoptosis was also almost entirely absent from the tumours in our experiment. TUNEL-stained liver sections revealed no increase in apoptotic cell nuclei after tamoxifen-induced deletion of Ctnnb1. Figure 3 shows representative non-apoptotic tumour tissue with residual GS-positive cells from an animal killed 3 weeks after tamoxifen treatment.
Cx32 levels in different tumour subpopulations
Immunohistochemical stainings were performed to analyse the localisation of Cx32, the major gap junction-forming protein in mouse liver, mediating gap junctional intercellular communication (GJIC) (Kumar and Gilula, 1996). Clearly reduced membranous Cx32 was found in GS-positive hepatomas from WT mice (Figure 4A), whereas hepatocytes from normal liver tissue exhibited physiological immunoreactivity for Cx32 in plaque-like structures at the cell membranes (Figure 4B). Interestingly, Cx32 appeared at cell membranes of GS-negative tumour subpopulations after KO of Ctnnb1 (Figure 4C and D).
Discussion
In the present work, we investigated the impact of a conditional KO of Ctnnb1 on the growth behaviour of mouse liver tumour cells with an activated Wnt/β-catenin signalling pathway.
In a recent study conducted by Ganzenberg et al (2013), tamoxifen-induced, Cre-mediated recombination of the Ctnnb1 gene led to β-catenin ablation in >99% of hepatocytes in livers from transgenic mice. Using the same mouse strain and treatment protocol, tamoxifen application was less effective in our experiment and the degree of Ctnnb1 KO varied considerably between different tumours. The reason for this phenomenon is not known. Transthyretin, the gene whose promoter was used to drive the expression of the Cre recombinase in our transgenic system, is not significantly altered in expression in Ctnnb1-mutated mouse liver tumours (Stahl et al, 2005) and hence altered expression of Cre is most likely not the underlying reason for incomplete recombination. Rather, we suspect that the different age of the mice at the time point of treatment might have had a role. Even more likely, pre-treatment with PB, an inducer of cytochrome P450 3A family enzymes involved in xenobiotic metabolism, might have contributed to the observed effect by inducing tamoxifen metabolism (Kivistö et al, 1998).
Nonetheless, although certainly of disadvantage for analyses in tissue homogenates, the coexistence of β-catenin-positive and -negative tumour cells allowed us a direct comparison of the two populations in individual tumours. Our results show that the proliferative index of GS-negative tumour cells was significantly lower than that of the neighbouring GS-positive tumour cells (Figure 2A). This suggests that β-catenin-dependent signalling has a role in regulating cell proliferation in these tumours. Malanchi et al (2008) showed that the survival of skin tumours crucially depends on the presence of active β-catenin: the KO of Ctnnb1 in murine skin tumours with aberrant β-catenin activation resulted in complete tumour regression within 6 weeks. By contrast, however, no indication for the death of hepatoma cells was observed in our analyses (Figure 3). Rather, the loss of β-catenin slowed down the proliferation of the tumour cells. Subsequently, the tumour volume fraction in livers from Ctnnb1 KO mice did not significantly change within a time period of 6 weeks (Figure 2B). This result points towards differences in the role of β-catenin-dependent signalling for the survival of tumour cells in liver and skin.
An important process involved in the regulation of cell proliferation is Cx32-mediated GJIC. Downregulation of GJIC often occurs during tumorigenesis and triggers proliferation of cancer cells (Chipman et al, 2003). Cx32, the major gap junction-forming protein in mouse liver, is absent from the membranes of Ctnnb1-mutated tumour cells (Moennikes et al, 2000; Marx-Stoelting et al, 2008). Our present results confirm that membranous Cx32 protein is decreased in GS-positive tumour cells (Figure 4A). After KO of Ctnnb1, Cx32 reappeared at the cell membrane of GS-negative subpopulations (Figure 4C). This indicates that β-catenin-dependent signalling has a role in Cx32-mediated GJIC. Furthermore, it has been reported that the inhibition of GJIC can make tumour cells independent from growth-restraining factors from neighbouring cells (Yamasaki and Naus, 1996; Chipman et al, 2003). This raises the possibility that there is a relationship between cell–cell communication and cell proliferation in the examined tumours. Reduced proliferation in the GS-negative hepatoma cell population could be a consequence of recurring Cx32-mediated GJIC. In tumours with more Cx32-positive cells, the remaining β-catenin-positive cells might receive stronger growth-restricting signals from surrounding cells, possibly leading to a stronger inhibition of cell proliferation.
In summary, the present results show that the ablation of β-catenin in mouse liver tumours leads to a significant attenuation of tumour cell proliferation but not to an induction of tumour cell death and hence not to tumour regression. Therefore, β-catenin-dependent signalling seems to be an important factor involved in the regulation of cell proliferation in this experimental system, but its loss is not sufficient to trigger cell death in those tumours.
In addition, GS-positive tumours from WT mice showed massive BrdU incorporation several weeks after the animals were set on a PB-free diet and the tumour volume fraction in respective livers significantly increased over time. This indicates that PB, as a proliferative stimulus, is only required at early stages of tumorigenesis but is no longer needed for maintenance of tumour growth in established Ctnnb1-mutated mouse liver tumours.
Acknowledgments
This work was supported by the Deutsche Forschungsgemeinschaft (grant SFB773). We acknowledge the excellent technical assistance by Johanna Mahr and Elke Zabinsky. We also thank Dr Sabine Colnot (Paris, France) who kindly provided the ApcloxP/loxP/TTR-Cre-Tam mice and Dr Joerg Huelsken (Lausanne, Switzerland) for the kind gift of Ctnnb1loxP/loxP mice.
The authors declare no conflict of interest.
Footnotes
This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License.
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