Table 2. Migration patterns of the Atg proteins on SDS-PAGE.

Protein	m calculateda (kDa)	m SDS-PAGEb (kDa)	Times higher on SDS-PAGE than calculated
Atg3	36	46	1.28
Atg3ΔFR	28	28	1
Atg3FR	10	20	2
ATG3	36	42	1.17

^a The calculated mass of the particular protein was obtained from its corresponding amino acid sequence (Fig. S3). ^bData for migration on SDS-PAGE are from Figure 4A.