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. 2013 Oct 8;8(12):e26664. doi: 10.4161/psb.26664

Nitric oxide mediates Cd-dependent induction of signaling- associated genes

Jagna Chmielowska-Bąk 1, Joanna Deckert 1,*
PMCID: PMC4091360  PMID: 24105301

Abstract

The first reaction of plants to stress factors, including cadmium, is activation of signal transduction pathways leading to the mobilization of defense mechanisms. In a recent study we have demonstrated that cadmium causes a rapid induction of several genes associated with cellular signaling in soybean seedlings. Here we show that nitric oxide can be an important signaling molecule mediating observed increase in genes expression under short-term cadmium stress.

Keywords: cadmium, heavy metal stress, gene expression, signaling, soybean

Cadmium is a heavy metal that exhibits deleterious effect in all organisms. The earliest response to this stress factor is activation of signal transduction pathways leading to the changes in genes expression crucial for acquisition of tolerance. In a recent study1 we have shown that cadmium causes increase in expression of several signaling-associated genes in the roots of soybean seedlings. A Cd-dependent induction of genes encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS), mitogen-activated protein kinase2 (MAPKK2), and DOF1 and MYBZ2 transcription factors was noted after 3 h, while 6 h-long treatment led to the increase in the levels of mRNA encoding nitrate reductase (NR), ACS, MYBZ2, and bZIP62 transcription factors.1 The obtained results raise the question about signaling molecules mediating the rapid Cd-dependent induction of analyzed genes.

One of the potential candidates is nitric oxide, an important signaling molecule involved in the regulation of the expression of numerous genes.2-4 Increase in NO levels in response to short-term cadmium stress has been observed in various plant species.4-8 Moreover, it has been shown that nitric oxide is engaged in stimulation of cadmium uptake,4-6 mediation of Cd-dependent programmed cell death,5,6 and regulation of antioxidant enzymes activity,9,10 reactive oxygen species levels,9 and phytochelatin synthesis10 in plants exposed to cadmium.

The results of the present study show that treatment with nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), reversed the observed increase in expression of all genes induced by cadmium stress in the roots of soybean seedlings after 3 h of treatment (Fig. 1A-D). Interestingly, in the case of genes induced after 6 h of cadmium application, this effect was not observed (Fig. 1E-H). In order to examine if the impact of PTIO on genes expression depended on changes in Cd uptake, Cd accumulation has been evaluated in the roots of soybean seedlings. Histochemical staining with Dithizone reagent showed that after 3 h of treatment PTIO slightly increased the amount of accumulated metal (Figs. 2A and 3A), while after longer treatment periods (6 h) it strongly attenuated Cd uptake (Figs. 2B and 3B). The obtained results suggest that, after short treatment periods (3 h), NO mediates Cd-dependent induction of several genes associated with signaling pathways.

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Figure 1. Relative expression of Cd-inducible genes after 3 (A-D) and 6 (E-H) hours of treatment with distilled water (control), 50μM PTIO, CdCl2 with cadmium in the concentration of 25 mg L-1 (corresponding to 223μM), or CdCl2 with cadmium in the concentration of 25 mg L-1 and 50μM PTIO. Genes expression has been measured with the use of real-time PCR technique. The results are means of 2–3 independent experiments ± SE.

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Figure 2. Accumulation of cadmium in the roots of soybean seedlings detected by histochemical staining with Dithizone reagent. The seedlings were treated with distilled water (control), 50 μM PTIO (PTIO), CdCl2 with cadmium in the concentration of 25 mg L-1 (Cd25), or CdCl2 with cadmium in the concentration of 25 mg L-1 and 50 μM PTIO (Cd25+PTIO) for 3 (A) or 6 (B) hours.

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Figure 3. Relative staining intensity of soybean seedlings roots. The seedlings were treated according to the description in Figure 2 for 3 (A) or 6 (B) hours. Densitometric quantification was performed with the use of Science Lab Software. The results are means of 2 independent experiments ± SE.

On the basis of presented research, earlier studies performed in our laboratory, and literature data, it can be concluded that early signaling events triggered by cadmium stress involve accumulation of NO11 mediating induction of ACS, MAPKK2, DOF1, and MYBZ2 genes. Exposure to Cd also causes rapid release of Ca2+ to cytoplasm12,13 leading to the activation of NADPH oxidase and generation of reactive oxygen species (ROS).12 All mentioned signaling molecules, NO, Ca2+, and ROS, are engaged in the stimulation of MAPK cascades observed in response to short-term cadmium stress on both transcription and post-transcription levels.1,14-17 Increase in ACS expression in turn leads to the induction of ethylene biosynthesis.1 The rapid response to Cd stress also includes stimulation of several transcription factors18 among others DOF1, MYBZ2, and bZIP62.1

Acknowledgments

The project was financed by the National Science Center (Poland) granted on the basis of decision number DEC-2011/03/N/NZ9/00214. Chmielowska-Bąk J is a scholarship holder within the project “Scholarship support for PhD students specializing in majors strategic for Wielkopolska’s development,” Sub-measure 8.2.2 Human Capital Operational Programme, co-financed by the European Union under the European Social Fund.

10.4161/psb.26664

Disclosure of Potential Conflicts of Interests

No potential conflicts of interests were disclosed.

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