Skip to main content
. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Immunity. 2014 May 22;40(6):865–879. doi: 10.1016/j.immuni.2014.03.014

Figure 6. Absence of Jarid2 in CD4+ T cells results in enhanced Th17 development.

Figure 6

(A) FACS analysis of IL-17A and IFN-γ expression in CD4+CD44+ cells from Jarid2fl/fl; CD4-cre mice and littermate controls cultured under Th17 polarizing conditions without IL-1β and (B) the corresponding percentages of CD4+CD44+IL-17A+ T cells. (C) Relative expression of Il17a, Il17f, Atf3, Rorc, Irf4, Batf mRNA by RT-qPCR from WT and Jarid2-deficient Th17 cultures. (D) Relative expression of Il22, Il9 and Il10 mRNA in Jarid2-deficient and WT Th17 cultures was determined by RT-qPCR. (E) Cytokine concentrations in supernatant of Th17 cultures were determined by ELISA. (F) H3K27me3 at promoters of Il22, Il10, Il9 and Hprt determined by ChIP-qPCR. (G) RT-qPCR of Il22 intron 4 and Il9 intron 3 of Jarid2-deficient and WT Th17 cultures ± reverse transcriptase (RT).