Skip to main content
PMC home page
Asian-Australasian Journal of Animal Sciences logoLink to Asian-Australasian Journal of Animal Sciences
. 2012 Apr;25(4):479–485. doi: 10.5713/ajas.2011.11435

Improvement of Fermentation and Nutritive Quality of Straw-grass Silage by Inclusion of Wet Hulless-barley Distillers’ Grains in Tibet

Xianjun Yuan, Chengqun Yu 1, M Shimojo 2, Tao Shao *
PMCID: PMC4092902  PMID: 25049588

Abstract

In order to develop methods that would enlarge the feed resources in Tibet, mixtures of hulless-barley straw and tall fescue were ensiled with four levels (0, 10%, 20%, and 30% of fresh weight) of wet hulless-barley distillers’ grains (WHDG). The silos were opened after 7, 14 or 30 d of ensiling, and the fermentation characteristics and nutritive quality of the silages were analyzed. WHDG addition significantly improved fermentation quality, as indicated by the faster decline of pH, rapid accumulation of lactic acid (LA) (p<0.05), and lower butyric acid content and ammonia-N/total N (p<0.05) as compared with the control. These results indicated that WHDG additions not only effectively inhibited the activity of aerobic bacteria, but also resulted in faster and greatly enhanced LA production and pH value decline, which restricted activity of undesirable bacteria, resulting in more residual water soluble carbohydrates (WSC) in the silages. The protein content of WHDG-containing silages were significantly higher (p<0.05) higher than that of the control. In conclusion, the addition of WHDG increased the fermentation and nutritive quality of straw-grass silage, and this effect was more marked when the inclusion rate of WHDG was greater than 20%.

Keywords: Wet Hulless-barley Distillers’ Grains, Hulless-barley Straw, Tall Fescue, Mixed Silage, Fermentation Quality

INTRODUCTION

The Tibetan plateau located in southwest China and other countries with an average altitude of over 4,000 m (Duan et al., 2008), is regarded as the Earth’s third pole and the highest unique territorial unit in the world, thus, its climate and natural environment are inherently extreme and instable. The cold and arid continental climates and short growing seasons are considered to be the most important limiting factors for forage production. Due to seasonal changes throughout the year, the shortage of feedstuffs has resulted in the fluctuation in animal production and therefore most of Tibet’s yak farmers feed their cows with agricultural by-products (mainly various straws) during the cool/cold season. However, the use of such straws has limitations due to their low nutritive value indicated by their high cellulose, hemicellulose and lignin contents, and their low protein content and digestibility. Many methods including alkali treatment, radiation, grinding, and pelleting have been used to exploit the nutrient utilization of poor quality roughages, but being expensive and difficult to apply, are not likely to be acceptable to small-holder dairy farmers in Tibet. Ensiling could be a better option for forage production, as it not only could improve nutritive value of straws, but could also facilitate year-round fodder provision and avoid nutrition loss (Titterton and Maasdorp, 1997).

Straws are too low in water soluble carbohydrates (WSC) for extensive fermentation to produce well-preserved silage (McDonald et al., 1991). Recently, rice straw and sugar-rich materials have been used as dairy cattle feed in the form of roughage mixtures (Suksombat, 2004). Supplement or ensiling with other palatable materials, especially green forages or agroindustrial by-products, has been used in many developed countries in Asia for improving the available nutritients for maintenance of animals. Ensiling has been found to enhance fermentation quality and to produce a maintenance diet for rams or cattle (Cao et al., 2011; Ridla and Uchida, 1994). In our previous studies, it was found that ensiling straw with grass slightly increased the moisture content and main fermentation substrates such as WSC, which are generally high in tall fescue.

In the Tibetan plateau, the history of making and drinking barley wine dates back several thousand years, and there is a substantial amount of wet hulless-barley distillers’ grains (WHDG) produced annually leading to an ever increasing interest in the efficient use of these WHDG due to economic and environmental concerns. WHDG usually have a low crude fiber, high crude protein and high digestibility, but high water content limits the length of storage. Because of storage problems, WHDG is often dried, however, artificial drying increases the price of WHDG substantially. Hence, most of WHDG are discarded or fresh fed for limited periods of time in Tibet. Previous researchers reported that combining and ensiling wet brewers grains (by-product very similar to WHDG) with drier feedstuffs improved its digestibility and aerobic stability compared with wet brewers grains ensiled alone (Anderson et al., 2009; Nishino et al., 2003). Ridla and Uchida (1994) reported that ensiling barley straw with wet brewers’ grains improved the fermentation quality and nutritive value of the mixture. Chiou et al. (2000) found that adding wet sorghum distillers’ grains to napiergrass silage improved the fermentation quality and produced a higher Flieg score. However, there is limited information regarding the effects of WHDG addition to mixed silage, especially in Tibet.

In our previous studies, the optimum straw-grass ratio was investigated by adding tall fescue to hulless-barley straw in varying proportions, increasing from 10 to 80%. The results indicated that the proportion of tall fescue should be at least 40%. The present experiment was intended to evaluate the effects of WHDG on the fermentation characteristics and nutritive value of straw-grass mixed silage. The aim was to preserve a forage resource in the crop harvest season by incorporating WHDG to establish a total forage programme for yak farmers in Tibet.

MATERIALS AND METHODS

Silage making

The hulless-barley (Hordeum vulgare) and tall fescue (Festuca arundinacea) were cultivated in the experimental field of the Grassland Station of Rikaze, Tibet. Both of these crops were harvested by hand on 27 Sep, 2009; the tall fescue was at the heading stage of growth. Hulless-barley straw and tall fescue were collected and chopped into approximately 2 cm lengths by a forage chopper and immediately transported to the laboratory. WHDG was obtained from a private barley wine processing company at Rikaze, Tibet in China and subjected to mixed silages preparation on the same day of production.

Hulless-barley straw and tall fescue were mixed in the proportions of 40/60 (wet weight), and WHDG (0, 10%, 20% or 30% wet basis) was added to the mixed silage. After a thorough mixing, 70 g of the mixture was packed into a laboratory silo (110 ml capacity), followed by being sealed with a screw top and kept at the ambient temperature. Triplicates silos were made for each treatment and each sampling day. The silos for each mixture were opened on 7, 14 and 30 d after ensiling.

Chemical analyses

Samples of hulless-barley straw, tall fescue and wet hulless-barley distiller’s grains were taken before ensiling for determination of dry matter (DM), water soluble carbohydrate (WSC), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF). After the silos were opened, the contents were mixed thoroughly, a 35 g sample was taken from each silo. Seventy g of distilled water was added to the 35 g sample and left at 4°C for 24 h, the extracts were then filtered through double-layered cheesecloth and a filter paper (Xinhua Co, China). The filtrates were used for determining pH, ammonia-N (AN), lactic acid (LA) and volatile fatty acids (VFAs) concentrations. The pH of the silage was measured with a glass electrode pH meter (HANNA pH 211, Hanna Instruments Italia Srl, Italy). The LA was determined by the method of Barker and Summerson (1941). VFAs were determined with gas chromatography (Shimadzu GC-17A, Japan, with 12 m capillary column, condition: column temperature 130°C, injection temperature 220°C). Ammonia-N was determined using the phenol-hypochlorite reaction method (Broderick and Kang, 1980). The DM contents of the fresh material and silages were determined by drying in an oven at 65°C for at least 48 h (AOAC, 1984). Total nitrogen (TN) was analyzed by the Kjeldahl procedure (Krishnamoorthy et al., 1982), CP was determined as the TN multiplied by 6.25. The WSC contents were determined by colorimetric after reaction with anthrone reagent (Thomas, 1977). The contents of NDF and ADF were determined according to Van Soest et al. (1991).

Statistical analyses

The statistical analysis included two-way analysis of variance with additive and storage periods as factors and Fisher’s least significant difference test. These were performed using the GLM procedure of SAS, with the level of statistical significance preset at p<0.05 (SAS, 1999).

RESULTS

The chemical compositions of hulless-barley straw, tall fescue and WHDG before ensiling are presented in Table 1. WHDG was high in CP and low in DM, NDF and ADF. The WSC content of tall fescue was relatively high compared to the hulless-barley straw and WHDG.

Table 1.

Chemical composition of hulless-barley straw, tall fescue and wet hulless-barley distillers’ grains before being ensiled

Ensilage materials Dry matter (g/kg FW) Crude protein (g/kg DM) WSC (g/kg DM) NDF (g/kg DM) ADF (g/kg DM)
Hulless-barley straw 353.38 134.10 102.68 513.02 270.55
Tall fescue 337.83 57.19 263.32 477.61 264.21
Wet hulless-barley distillers’ grains 155.86 259.47 92.21 438.21 107.94
Open in a new tab

FW = Fresh weight, WSC = Water soluble carbohydrate, NDF = Neutral detergent fiber, ADF = Acid detergent fiber.

Changes in pH, DM and LA contents of silages are presented in Table 2. The fermentation was more pronounced up to d 7 and then it slowed down in all the WDHG addition treatments. All WHDG addition silages showed basically a similar pH profile, which was significantly lower than that of the control (p<0.05). The pH value tended to decrease with increasing inclusion of WHDG (p>0.05), silage containing 30% WHDG had the lowest pH value (4.19), whereas that of the control was still 4.88 after 30 d of ensiling. LA was produced rapidly during the first 7 d in WHDG addition silages, with the LA content was more than double that of the control silage, and this difference was maintained up to 30 d of ensiling (p<0.05). The highest LA content was observed in the silages with 20% WHDG inclusion. The DM content decreased as the rate of inclusion of WHDG increased (p<0.05), and decreased slightly (p>0.05) from the 7th d of ensiling, reaching the lowest (p<0.05) at the end of ensiling (d 30).

Table 2.

Effect of adding WHDG on pH, DM and LA content of mixed silage of hulless-barley straw and tall fescue during ensiling

Items Ensiling days Control 10% WHDG 20% WHDG 30% WHDG
DM (g/kg FW) 7 346.62±5.08aA 332.69±9.67aB 317.90±4.80aC 305.98±1.34aD
14 333.01±3.00abA 321.28±7.30aB 310.92±1.17abC 300.41±1.16aD
30 318.95±11.03bA 315.63±17.91aA 300.16±8.36bAB 288.45±5.00bB
pH value 7 5.76±0.18aA 4.38±0.12aB 4.32±0.03aB 4.20±0.03aB
14 5.09±0.14bA 4.26±0.13aB 4.15±0.06bB 4.10±0.03bB
30 4.88±0.13bA 4.44±0.01aB 4.24±0.03aC 4.19±0.06aC
Lactic acid (g/kg DM) 7 22.70±4.24cC 50.33±5.89aB 62.34±2.53aA 66.45±1.06aA
14 39.42±6.03bC 55.10±4.43aB 64.45±6.40aAB 65.89±5.70aA
30 56.29±2.42aBC 52.53±5.27aC 70.37±8.54aA 64.11±5.04aAB
Open in a new tab

WHDG = Wet hulless-barley distillers’ grains. Average means±standard deviations.

Values with different lower case letters show significant differences among ensiling days in the same treatment.

Values with different capital letters show significant differences among treatments in the same ensiling day (p<0.05).

Acetic acid (AA) concentration increased gradually in all silages during the ensiling period (Table 3). WHDG addition silages showed somewhat (p>0.05) or significantly (p<0.05) higher AA content than the control, and the silage inclusion with 10% WHDG had the highest AA concentration during the full fermentation course. Changes in the total VFAs concentration showed a similar trend to the AA concentrations. Propionic acid and butyric acid concentrations tended to increase with prolonged storage, and were significantly (p<0.05) lower in silages containing WHDG as compared to the control (Table 3). The ratio of LA/AA in WHDG-containing silages reached a peak on day 7 of ensiling, and then gradually decreased until the end of the ensiling period (d 30). In contrast, the ratio of LA/AA in the control silage increased during the ensiling period and by the end of the ensiling period (d 30) was significantly (p<0.05) higher as compared to the WHDG-containing silages.

Table 3.

Effect of adding WHDG on volatile fatty acid content of mixed silages of hulless-barley straw and tall fescue during ensiling

Items Ensiling days Control 10% WHDG 20% WHDG 30% WHDG
Acetic acid (g/kg DM) 7 15.74±1.37bB 23.31±2.55bA 1.45cB±18.57 17.07±1.63bB
14 22.29±3.05aB 49.10±6.65aA 24.43±1.03bB 25.13±3.03aB
30 18.01±0.89bC 39.80±4.59aA 28.36±1.49aB 31.82±4.89aB
Propionic acid (g/kg DM) 7 7.26±0.60aA 0.33±0.18aB 0.42±0.02bB 0.54±0.11bB
14 9.87±1.43aA 0.38±0.21aB 0.44±0.19bB 0.20bB±0.59
30 8.20±2.42aA 0.66±0.19aB 1.44±0.18aB 1.13±0.09aB
Butyric acid (g/kg DM) 7 5.84±0.50aA 0.70±0.13bB 0.53±0.04aB 0.38±0.06aB
14 7.63±2.05aA 0.46±0.10bB 0.44±0.26aB 0.61±0.16aB
30 6.41±1.98aA 1.28±0.16aB 0.45±0.11aB 0.89±0.50aB
Total VFAs (g/kg DM) 7 28.84±0.28bA 25.23±2.28bB 20.03±1.41cC 17.99±1.75cC
14 39.78±3.84aB 50.33±6.88aA 25.31±1.34bC 26.33±3.20bC
30 32.61±5.16abB 41.95±4.41aA 31.25±1.46aB 34.31±4.19aAB
Lactic acid/Acetic acid 7 1.43±0.16bD 2.17±0.28aC 3.36±0.12aB 3.92±0.40aA
14 1.77±0.18bB 1.14±0.18bC 2.64±0.21bA 2.66±0.52bA
30 3.13±0.25aA 1.62±0.58abC 2.48±0.32bAB 2.04±0.33bcBC
Open in a new tab

WHDG = Wet hulless-barley distillers’ grains. Average means±standard deviations.

Values with different lower case letters show significant differences among ensiling days in the same treatment.

Values with different capital letters show significant differences among treatments in the same ensiling day (p<0.05).

The ratio of AN/TN slightly increased (p>0.05) during the first 14 d of ensiling and then significantly increased (p<0.05) at 30 d of ensiling (Table 4). The ratio of AN/TN in the control silage (163.88) was nearly three times that of the WHDG-containing silages at d 7 of ensiling and this difference was maintained for the rest of the duration of the ensiling period (p<0.05). The residual WSC decreased (p<0.05) over the first 14 d of ensiling from the original values of fresh forage, and then slowly declined until the end of the ensiling period (Table 4). At the 7th d of ensiling the control silage showed significantly (p<0.05) higher WSC content than WHDG-containing silages, however, this difference was reversed by the end of the ensiling period (p<0.05).

Table 4.

Effect of adding WHDG on WSC content and AN/TN of mixed silages of hulless-barley straw and tall fescue during ensiling

Items Ensiling days Control 10% WHDG 20% WHDG 30% WHDG
AN/TN (g/kg TN) 7 163.88±8.43bA 49.19±2.85bB 43.21±1.58bB 41.15±1.83bB
14 169.02±3.47bA 56.96±6.66abB 48.25±2.70bC 45.69±1.48bC
30 193.21±10.31aA 7.09aB±61.41 77.41±9.38abB 69.87±7.99aB
WSC (g/kg DM) 7 70.69±4.64aA 44.86±3.83aB 36.69±2.58aC 34.05±1.57aC
14 24.72±1.34bA 22.65±3.43bA 21.27±1.57bA 21.30±5.43bA
30 13.27±0.95cB 18.28±5.11bA 17.70±0.35cAB 18.26±0.41Ba
Open in a new tab

WHDG = Wet hulless-barley distillers’ grains. Average means±standard deviations.

Values with different lower case letters show significant differences among ensiling days in the same treatment.

Values with different capital letters show significant differences among treatments in the same ensiling day (p<0.05).

The chemical compositions of the silages after 30 d of ensilage are presented in Table 5. The addition of WHDG to silage resulted in a significant increase (p<0.05) in CP contents, with the silage containing 30% WHDG having the highest CP content, however, when the inclusion level of WHDG increased from 10% to 20% there was no effect (p>0.05) on the CP content of the silage. While WHDG addition tended to decrease NDF and ADF content of mixed silages (p>0.05), silage containing 30% WHDG had the lowest NDF content (p<0.05) and when the inclusion level of WHDG was above 20% the ADF content was significantly higher (p<0.05) than that of the control.

Table 5.

The chemical composition of mixed silage of hulless-barley straw and tall fescue after 30 days ensiling

Item Control 10% WHDG 20% WHDG 30% WHDG
Crude protein (SD) (g/kg DM) 88.97±2.93C 103.41±1.00B 106.43±1.09B 114.62±2.37A
NDF (SD) (g/kg DM) 607.46±23.56A 599.14±2.72A 579.60±7.30A 539.46±18.49B
ADF (SD) (g/kg DM) 361.70±9.39A 338.58±6.96AB 328.17±31.37B 316.21±11.09B
Open in a new tab

WHDG = Wet hulless-barley distillers’ grains. Average means±standard deviations.

Values with different capital letters show significant differences among treatments (p<0.05).

DISCUSSION

After 30 d of ensiling, the pH of silages containing 10 or 20% WHDG was not below 4.2, which is a target pH for well-preserved silage, however, this critical pH value varies with moisture content (McDonald et al., 1991). According to Weissbach (1996), when the DM content of an ensiled crop was 320 g/kg, a pH of 4.53 could ensure effective preservation. Meeske et al. (2002) also reported that oat silages were well preserved at a pH of 4.56 and 4.52 when the DM was 317 and 328 g/kg, respectively. In this study, the DM content of the WHDG-containing silages was above 303.01 g/kg, and the pH value of these silages was lower than 4.4, while that of control silage was 4.88, suggesting that the WHDG-containing silages had effective preservation. Other indicators of effective preservation include AN being less than 100 g/kg TN (Haigh and Hopkins, 1977), butyric acid lower than 10 g/kg DM (Nilsson and Nilsson, 1956), and an efficient conversion of WSC to lactic acid (Parker and Crawshaw, 1982). On the basis of these parameters the mixed silages containing WHDG were well preserved, especially the silages containing 20 or 30% WHDG.

The DM contents of the WHDG-containing silages were significantly lower compared with the control and decreased as the inclusion rate of WHDG increased (p<0.05). This was due to the high moisture content of WHDG.

At 7 d of ensiling, the pH value of all the WHDG-containing silages was lower than 4.38, while that of the control silage was still 5.76. Correspondingly, the LA concentration of the WHDG-containing silages was significantly higher (p<0.05) than that of the control silage. After 7 d, WHDG-containing silages reached a stable condition, with the pH value and the LA concentration remaining almost unchanged until the fermentation was terminated at 30 d. The WHDG-containing silages showed more extensive fermentation than the control silage, especially during the first 7 d of ensiling. Although there was a gradual increase in LA concentrations in the control silage after 7 d of ensiling, the pH value and LA concentrations remained significantly different (p<0.05) between the control silage and 20% or 30% WHDG-containing silages. This indicated that WHDG addition accelerated the onset of LA fermentation. There may be several reasons for this. The inclusion of WHDG would have a dual action in the mixed silage. Firstly, there were large amounts of O2 trapped in silos and aerobic bacteria could dominate the fermentation during the initial period of ensiling, however, residual ethanol contained in WHDG not only inhibited the activity of undesirable (aerobic) microorganisms, but also accelerated the onset of LA fermentation. On the other hand, the higher moisture content contained in WHDG also could stimulate the growth of LA-producing bacteria and accelerate the rate of fermentation. Zhang et al. (2011) reported that ethanol addition could inhibit the activity of aerobic microorganisms and depress the losses of WSC during early stages of ensiling and result in more WSC for LA production in elephant grass silage. This result is also consistent with the reports of Ridla and Uchida (1994), who observed that fermentation was stimulated and rapidly achieved acid condition by mixing barley straw with wet brewers’ grains.

The concentration of AA increased with the ensiling period, and the ratio of LA/AA tended to decline in the WHDG-containing silages after 7 d of ensiling. This indicated that the fermentation pattern was changed from homofermentation to heterofermentation and is in agreement with other studies as reported by Shao et al. (2002; 2005), who found that there was a significant shift from homofermentative to heterofermentative activity after 5 d of ensiling. McDonald et al. (1991) also reported that in well-preserved silages, acidification was initiated by homofermentative strains, but after only 4 d 85% of the strains were heterofermentative, whose tolerance to acetic acid was greater than homofermentative strains. The WHDG-containing silages showed somewhat (p>0.05) or significantly (p<0.05) higher AA concentrations as compared with the control silage, with the silage containing 10% WHDG having the highest AA concentrations throughout the ensiling period (p<0.05). This may be explained as follows. WHDG probably contained some AA-producing bacteria, the majority of which were aerobes. These microbes can utilize fructose and glucose as energy sources via the phosphate pentose pathway, with acetaldehyde as an intermediate, producing acetic acid before complete exhaustion of fresh air from the silage mass (McDonald et al., 1991). However, ethanol could inhibit the activity of aerobic microorganism (including AA-producing bacteria), and these effects may become more pronounced as the proportion of WHDG increased in the silage mixture.

The ratio of AN/TN in silages indicated the extent of proteolysis. Kemble (1956) reported that proteolysis began immediately at harvest, with the majority occurring during the first week of ensiling. Our observations are consistent with this, the proteolysis-inhibited effect of WHDG in the silage mixtures was pronounced during the ensiling especially the initial stages of ensiling. It is generally recognized that protein is degraded into amino acids by microorganisms and plant enzymes, and further broken down into ammonia or amines, resulting in a loss of silage protein. In the present study, the ratio of AN/TN in the WHDG-containing silages was significantly lower than the control silage after 7 d. This may be due to ethanol contained in WHDG inhibiting the degradation of protein by aerobic bacteria during the early stages of ensiling. Zhang et al. (2011) found that ethanol could act as a silage fermentation inhibitor to effectively reduce the ammonia-N content of silage. The significant difference of butyric acid concentration between WHDG-containing and the control silages also indicated that the activity of clostridial organisms (which produce butyric acid) was inhibited by residual ethanol of WHDG. Chiou et al. (2000) also reported that including wet sorghum distillers’ grains in silage made from Napier grass significantly lowered ammonia-N and butyric acid concentration as compared to the control.

The content of residual WSC in WHDG-containing silages was significantly lower than that of the control silage, and declined with the increasing levels of WHDG within 7 d of ensiling. This indicated that the WHDG-containing silages had more extensive fermentation than the control silage during the first 7 d of ensiling, and more WSC was consumed by the LA-producing bacteria. This result is consistent with higher concentrations of LA in the WHDG-containing silages. Ultimately, the concentrations of WSC and LA were significantly higher in the silages containing 10 and 30% WHDG as compared to the control silage indicating that WHDG improved the transformation of WSC to LA and AA. This may have been due to residual ethanol contained in WHDG depressing the loss of WSC consumed by undesirable bacteria, so that more residual WSC was present in the final silages resulting in a greater amount of substrate available for LA-producing bacteria. This is in agreement with Ohba et al. (2002), who observed that 3% ethanol promoted a more efficient fermentation, as indicated by a faster pH decline, higher concentration of LA and residual WSC. Chiou et al. (2000) also demonstrated that increased levels of inclusion of wet sorghum distillers’ grains in Napier grass silage not only increased the WSC content but also increased the titratable acidity, providing a better fermentation environment for LA-production bacteria.

The difference between the control and WHDG-containing silages with regard to chemical composition depended on the chemical characteristics of the WHDG added. The significantly higher CP concentrations in the WHDG-containing silages compared with the control silage are consistent with the inclusion of WHDG, which is higher in CP than hulless-barley straw and tall fescue. The addition of WHDG to mixed silage resulted in decreased ADF and NDF contents, due to the low ADF and NDF content in WHDG. These results are in agreement with Ridla and Uchida (1994) who found significant increase in silage CP content and reduction in fibrous components with increasing proportion of wet brewers’ grains.

CONCLUSIONS

In conclusion, in this study WHDG inclusion accelerated the initiation LA fermentation and inhibited the activity of aerobic bacteria, which could compete with desirable LA-producing bacteria for WSC and protein. The addition of WHDG not only improved fermentation quality of mixed silage of hulless-barley straw and tall fescue, but also increased their CP content and decreased the ADF and NDF contents. Therefore, addition of WHDG to mixed silage of hulless-barley straw and tall fescue can be a good approach toward solving the forage shortage during the insufficiency periods of forage in Tibet.

ACKNOWLEDGEMENTS

This work was supported by the grant for key techniques research in straw-grass mixed silage from Tibet (XZ20093ZD) and the grant of National “12th Five-Year” Plan for Science and Technology Support project: Comprehensive treatment key technology and demonstration project of the Degraded grassland in north Tibetan Plateau (2010BAE00739-03).

REFERENCES

  1. Anderson JL, Kalscheur KF, Garcia AD, Schingoethe DJ, Hippen AR. Ensiling characteristics of wet distillers grains mixed with soybean hulls and evaluation of the feeding value for growing Holstein heifers. J Anim Sci. 2009;87(6):2113–2123. doi: 10.2527/jas.2008-1607. [DOI] [PubMed] [Google Scholar]
  2. AOAC. Official methods of Analysis. 14th ed. Association of Official and Analytical Chemists; Arlington, Virginia, USA: 1984. [Google Scholar]
  3. Barker SB, Summerson WH. The colorimetric determination of lactic acid in biological material. J Biol Chem. 1941;138:535–554. [Google Scholar]
  4. Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci. 1980;63(1):64–75. doi: 10.3168/jds.S0022-0302(80)82888-8. [DOI] [PubMed] [Google Scholar]
  5. Cao Y, Cai Y, Hirakubo T, Fukui H, Matsuyama H. Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons. Anim Sci. 2011;82(2):259–266. doi: 10.1111/j.1740-0929.2010.00840.x. [DOI] [PubMed] [Google Scholar]
  6. Chiou PWS, Chang SH, Yu B. The effects of wet sorghum distillers’ grains inclusion on napiergrass silage quality. J Sci Food Agric. 2000;80(8):1199–1205. [Google Scholar]
  7. Duan YH, Tan ZF, Wang YP, Li ZW, Li ZY, Qin GY, Huo YP, Cai YM. Identification and characterization of lactic acid bacteria isolated from Tibetan Qula cheese. J Gen Appl Microbiol. 2008;54(1):51–60. doi: 10.2323/jgam.54.51. [DOI] [PubMed] [Google Scholar]
  8. Haigh PM, Hopkins JR. Relationship between oven and toluene dry matter in grass silage. J Sci Food Agric. 1977;28(6):477–480. [Google Scholar]
  9. Kemble AR. Studies on the nitrogen metabolism of the ensilage process. J Sci Food Agric. 1956;7(2):125–130. [Google Scholar]
  10. Krishnamoorthy U, Muscato TV, Sniffen CJ, Van Soest PJ. Nitrogen fractions in selected feedstuffs. J Dairy Sci. 1982;65(2):217–225. [Google Scholar]
  11. McDonald P, Henderson AR, Heron SJE. The biochemistry of silage. 2th ed. Cambrian Printers Ltd; Aberystwyth: 1991. [Google Scholar]
  12. Meeske R, van der Merwe GD, Greyling JF, Cruywagen CW. The effect of adding an enzyme containing lactic acid bacterial inoculant to big round bale oat silage on intake, milk production and milk composition of Jersey cows. Anim Feed Sci Technol. 2002;97(3–4):159–167. [Google Scholar]
  13. Nilsson G, Nilsson PE. Silage studies. IV. The microflora on the surface of some fodder plants at different stages of maturity. Arch Microbiol. 1956;24(4):412–422. doi: 10.1007/BF00693107. [DOI] [PubMed] [Google Scholar]
  14. Nishino N, Hiroaki H, Sakaguchi E. Evaluation of fermentation and aerobic stability of wet brewers’ grains ensiled alone or in combination with various feeds as a total mixed ration. J Sci Food Agric. 2003;83(6):557–563. [Google Scholar]
  15. Ohba N, Tobisa M, Shimojo M. Effect of ethanol addition on ensiling of forage oats and italian ryegrass. Sci Bull Faculty Agric Kyushu Univ. 2002;57:11–15. [Google Scholar]
  16. Parker JWG, Crawshaw R. Effects of formic acid on silage fermentation, digestibility, intake and performance of young cattle. Grass Forage Sci. 1982;37(1):53–58. [Google Scholar]
  17. Ridla M, Uchida S. Fermentation quality and silage additives value of barley straw and wet brwers’grains silage. Asian-Aust J Anim Sci. 1994;7:517–522. [Google Scholar]
  18. SAS. SAS/STAT user’s guide: (Version 8.01th ed) SAS Institute Inc; Cary, North Carolina, USA: 1999. [Google Scholar]
  19. Shao T, Ohba N, Shimojo M, Masuda Y. Dynamics of early fermentation of Italian ryegrass (Lolium multiflorum Lam.) silage. Asian-Aust J Anim Sci. 2002;15:1606–1610. [Google Scholar]
  20. Shao T, Zhang ZX, Shimojo M, Wanga T, Masuda Y. Comparison of fermentation characteristics of italian ryegrass (Lolium multiflorum Lam.) and guineagrass (Panicum maximum Jacq.) during the early stage of ensiling. Asian-Aust J Anim Sci. 2005;18:1727–1734. [Google Scholar]
  21. Suksombat W. Silage from agricultural by-products in Thailand: processing and storage. Asian-Aust J Anim Sci. 2004;17:473–478. [Google Scholar]
  22. Thomas TA. An automated procedure for the determination of soluble carbohydrates in herbage. J Sci Food Agric. 1977;28(7):639–642. [Google Scholar]
  23. Titterton M, Maasdorp BV. Nutritional improvement of maize silage for dairying: mixed crop silages from sole and intercropped legumes and a long season variety of maize. 2. Ensilage Anim Feed Sci Technol. 1997;69(1–3):263–270. [Google Scholar]
  24. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991;74(10):3583–3597. doi: 10.3168/jds.S0022-0302(91)78551-2. [DOI] [PubMed] [Google Scholar]
  25. Weissbach PJ. New developments in crop preservation. Proceedings of 11th International Silage Conference; Aberystwyth. 1996. [Google Scholar]
  26. Zhang L, Yu CQ, Shimojo M, Shao T. Effect of different rates of ethanol additive on fermentation quality of napiergrass (pennisetum purpureum) Asian-Aust J Anim Sci. 2011;24:636–642. [Google Scholar]

Articles from Asian-Australasian Journal of Animal Sciences are provided here courtesy of Asian-Australasian Association of Animal Production Societies (AAAP)

RESOURCES