Figure 3.

Integrin α and β expression in KTC^par and KTC^res cells. A depicts the FACS results given as mean fluorescence units. Tumor cells were washed in blocking solution and then stained with specific mAbs as listed in Materials and Methods section. To evaluate background staining of PE-conjugated antibodies, goat anti-mouse IgG1-PE or IgG2a-PE was used. Fluorescence was measured using a FACScan flow cytometer. * indicates significant difference between the resistant and the sensitive tumor subline. (B) Modification of intracellular integrin protein level. Tumor cell lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and blotted on the membrane incubated with appropriate mAbs. β-Actin served as the internal control. The figure shows one representative from three separate experiments.