Fig. 3.

Adenovirus based vaccine against Clostridium difficile toxin A is able to induce robust TA-specific T cell responses. WT BALB/c mice were IM injected (10¹⁰ vp/mouse) with Ad5-C. difficile-TA (n = 5). Mice were sacrificed at 14 dpi splenocytes prepared, and individually stimulated with pool of 12 peptides from the TA peptide library (each peptide 0.2 μg/well) and (A) IFNγ or (B) IL-2 ELISPOT was performed as described in Section 2. Naïve (n = 5) mice were stimulated with same individual peptides as baseline control. One of two representative experiments shown. Bars represent mean ± SD. Statistical analysis was completed using Two-Way ANOVA with a Bonferroni post hoc test (stimulations × treatments), p < 0.05 was deemed a statistically significant difference. *, **Statistically different from those in naïve mice (for the same stimulation), p < 0.05, p < 0.001, respectively.