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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Hypertension. 2011 Aug 1;58(3):431–438. doi: 10.1161/HYPERTENSIONAHA.111.172338

Figure 4.

Figure 4

Effect of acute Pin1 inhibition on vascular endothelial NO synthase (eNOS) function. Isolated aortas from control mice were treated with either juglone (A and B: 10 μmol/L; C: 0.1 to 10.0 μmol/L) or vehicle for 30 minutes. A, Representative Western blot showing eNOS Ser116 phosphorylation. B, NO production as measured by Nω-nitro-l-arginine (LNNA)–sensitive 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) fluorescence. C, Relaxation responses to acetylcholine after contraction with phenylephrine. Relaxation to 100% represents a full return to a passive tension of 0.75 g. Results are expressed as mean±SEM (n=3 independent experiments for A and n=6 to 7 aortas in each group for B and C). *P<0.05 vs control.