. Author manuscript; available in PMC: 2014 Sep 1.

Published in final edited form as: Glia. 2013 Jul 8;61(9):1456–1470. doi: 10.1002/glia.22534

Table 2. Quantitative PCR analysis demonstrates robust expression of Klk6, PAR1 and PAR2 by murine primary and Oli-neu oligodendrocytes.

The mean number of RNA copies encoding for Klk6, PAR1 and PAR2 RNA in 0.5 μg of total RNA isolated from immediately post-purification OPCs, mature oligodendrocytes (3DIV), undifferentiated (Oli-neu) or differentiated Oli-neu (Oli-neu + dbcAMP) oligodendrocytes is provided (± SEM). Amplification of GAPDH was carried out to verify equal loading.

	RNA Copy Number

Gene	Primary Oligodendrocyte Progenitor Cell	Primary Differentiated Oligodendrocyte	Oli-neu	Oli-neu + dbcAMP

Klk6	6.0E+03 (± 2.2E+02)	5.6E+03 (± 8.7E+01)	6.4E+04 (± 2.2E+04)	2.0E+04 (± 4.0E+03)
PAR1	1.1E+06 (± 5.7E+04)	8.6E+05 (± 3.6E+04)	1.5E+06 (± 2.2E+05)	1.3E+06 (± 3.3E+05)
PAR2	2.5E+02 (± 2.3E+01)	2.9E+02 (± 2.1E+01)	3.1E+03 (± 1.6E+02)	3.5E+03 (± 2.3E+01)
GAPDH	6.0E+06 (± 1.3E+05)	6.5E+06 (± 2.9E+05)	2.4E+07 (± 6.7E+05)	1.8E+07 (± 2.9E+05)