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. 2014 Apr 8;11:29. doi: 10.1186/1742-4690-11-29

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Copyright © 2014 Schaller et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SAMHD1_WTand SAMHD1_K11Abind Vpx_MACwith similar efficiencies. 293 T cells were co-transfected with plasmids expressing GST, GST-SAMHD1 or GST-SAMHD1_K11A along with plasmids expressing Flag-tagged Vpx derived from HIV-2_ROD, SIV_MAC or SIV_RCM. GST-pull down was performed and samples were separated using 15% SDS-PAGE gels. Vpx proteins were detected using an anti-Flag antibody, and SAMHD1 was detected using an anti-GST antibody. Tubulin served as a loading control for the input.