Table 3. Fluorescence Quenching of MftR and Mutants with the Addition of Urate.
K (μM) | nH | |
---|---|---|
MftR | 6.1 ± 2.1 | 0.8 ± 0.1 |
W11F | 2.4 ± 2.0 | 0.7 ± 0.2 |
D56S | 8.4 ± 2.5 | 0.8 ± 0.2 |
R63S | 6.6 ± 0.5 | 2.9 ± 1.3 |
R89N | 4.8 ± 2.0 | 1.0 ± 0.2 |
K (μM) | nH | |
---|---|---|
MftR | 6.1 ± 2.1 | 0.8 ± 0.1 |
W11F | 2.4 ± 2.0 | 0.7 ± 0.2 |
D56S | 8.4 ± 2.5 | 0.8 ± 0.2 |
R63S | 6.6 ± 0.5 | 2.9 ± 1.3 |
R89N | 4.8 ± 2.0 | 1.0 ± 0.2 |