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. Author manuscript; available in PMC: 2015 Jan 3.
Published in final edited form as: Chembiochem. 2013 Nov 20;15(1):135–145. doi: 10.1002/cbic.201300375

Figure 2.

Figure 2

Binding of Cy5-GLP-1/Glb 9 to βTC3 A) and INS-1F cells B & C). Cells were incubated with 25 nm ligand for 1 min then washed with images acquired 2 min following the wash. Surface plots to the right of each image show fluorescence intensity (y-axis) at each pixel within the image. At 25 nm ligand, fluorescence is high on the βTC3 cells A) but low for the INS-1F B), while at 250 nm ligand binding to the INS-1F cells is greatly increased C). Since the INS-1F cells only express the SUR1, the observation of binding only at 250 nm indicates that both receptors are required to bind significant ligand at lower concentrations; i.e., 25 nm.