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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Immunobiology. 2014 Apr 6;219(9):653–660. doi: 10.1016/j.imbio.2014.03.020

Fig. 4.

Fig. 4

The role of CSL and Notch1 in regulating Akt phosphorylation. (A) The RAW264.7 cell line was transfected with the control scrambled siRNA or notch1-specific siRNA. After 24 h of transfection, the cells were stimulated with LPS (100 ng/ml) for the indicated time, and phosphorylated and total Akt were detected using Western blotting. (B) BMMs from CSL conditional knockout mice were stimulated with LPS (100 ng/ml) for the indicated times, and the protein lysates were harvested to investigate the phosphorylation status of Akt.