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. 2014 Jun 19;4(2):566–584. doi: 10.3390/biom4020566

Figure 1.

Figure 1

Effect of PA28α, PA28αβ and PA28β on the chymotrypsin-like activity of 20S immunoproteasome. Assays were carried out in continuo at 37 °C in 500 μL of buffer reaction 20 mM Tris-HCl pH 7.5, 0.2% (w/v) BSA, containing 100 μM of Suc-LLVY-AMC. For each assay, 4 ng of 20S were used.