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. 2014 Jun 16;3(3):109–114. doi: 10.1002/open.201402002

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© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Unfolding kinetics of Cyt c monitored by time-resolved mass spectrometry. A) The kinetic profile of acid induced denaturation of Cyt c. The depletion of folded Cyt c () and the formation of unfolded species () is demonstrated. The plot was generated by measuring the signal intensity of charge states 7–20⁺ at different time points after mixing Cyt c with 5.0 % AcOH. Error bars represent the average error of three independent experiments. Solid lines are fits to the experimental data. B) Representative ESI-MS spectra recorded at different time points, 1.06, 2.14 and 5.95 s, during unfolding.

Inline graphic — Unfolding kinetics of Cyt c monitored by time-resolved mass spectrometry. A) The kinetic profile of acid induced denaturation of Cyt c. The depletion of folded Cyt c () and the formation of unfolded species () is demonstrated. The plot was generated by measuring the signal intensity of charge states 7–20⁺ at different time points after mixing Cyt c with 5.0 % AcOH. Error bars represent the average error of three independent experiments. Solid lines are fits to the experimental data. B) Representative ESI-MS spectra recorded at different time points, 1.06, 2.14 and 5.95 s, during unfolding.