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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2011 Aug 25;419(1):24–42. doi: 10.1016/j.virol.2011.07.017

Fig. 11.

Fig. 11

RhA3A does not inhibit retrotransposition. One μg of L1 constructs containing eGFP with or without the γ-globin intron in the opposite orientation and 200 ng of empty vector, hA3A, rhA3A or rhA3AΔSVR plasmid were co-transfected into 293T cells using Fugene 6 (Roche). At 4 days post-transfection, cells were harvested and analyzed for eGFP expression using a FACSCalibur machine (BD Biosciences) with 150,000 events. Panel A. Results of co-transfection of LINE 1 plasmid (with intron) with either the empty vector, hA3A or rhA3A plasmids. To minimize autofluorescence background, the percentage of eGFP+ cells from the untransfected control were gated against an empty fluorescence channel (FL3). Panel B. Results of co-transfection of eGFP control plasmid (no introns) with either the empty vector, hA3A or rhA3A plasmids.