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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2011 Aug 25;419(1):24–42. doi: 10.1016/j.virol.2011.07.017

Fig. 8.

Fig. 8

Sensitivity of rhA3A to lentivirus Vif-mediated degradation. Panel A. SHIV Vif mediates rhA3A degradation. SHIV and SHIVΔvif molecular clones were co-transfected with rhA3G, hA3A, rhA3A or rhA3AΔSVR. At 24 h post-transfection, cells were lysed and proteins were precipitated using methanol. Proteins were separated on a 12%-SDS-PAGE gel and probed using an antibody directed against the HA-tag. All samples were normalized to the amount of β-actin. Panel B. HIV-1 Vif mediates rhA3A degradation. HIV-1 and HIV-1Δvif molecular clones were co-transfected with rhA3G, hA3A, rhA3A and rhA3AΔSVR. In both panels, the blots were stripped and re-probed with an antibody against β-actin. All assays were performed twice.