Fig. 8.

GX-sPLA₂ deficiency increases T cell recruitment and immunoglobulin heavy chain mRNA expression in the lung tissue of mice during influenza infection. Day 0 and 3 pi with H1N1pdm (A/Mexico/4108/2009) influenza the lungs from GX^+/+ and GX^−/− mice (C3H/HeN background mice) were perfusion fixed in situ with 4% paraformaldehyde, sectioned and subject to immunofluorescence analysis with specific rabbit primary antibody against mouse CD3 antigen (marker for T-cell) (Ai). Representative images (at ×400 with 3.4 zoom factor) from five independent experiments are shown. Assessment of CD3 positive cells per high power field for day 0, 3, 6 and 14 days following infection with H1N1pdm influenza is shown (Aii). IgG (B) and CD8A (C) mRNA expression normalized to GAPDH were determined by quantitative real-time PCR in lung tissue of GX^+/+ and GX^−/− mice after H1N1pdm influenza infection. Scale bar: 10 μm. a, p<0.05 GX^+/+ or GX^−/− vs. base; b, p<0.05 GX^+/+ vs. GX^−/− at any time point, ANOVA followed by paired t-test, two tailed, assuming unequal variance. n≥8 per group. All the mice used in these experiments were genotyped littermates and grouped and analyzed by their genotype.