Table 1.
Analysis of mutations in VL segments of medaka immunoglobulin light chain coding ESTs
| EST | Germline VL |
Mutations |
FR | FR-PMb | Mutations |
CDR | CDR-PMb | |||
|---|---|---|---|---|---|---|---|---|---|---|
| FR1 | FR2 | FR3 | R:Sa | CDR1 | CDR2 | R:Sa | ||||
| OLOVA49021 | V3-2 | 0 | 3 | 1 | 3:1 | 0.115 | 2 | 1 | 1:2 | 0.299 |
| OLKI56H12 | V5-2 | 3 | 0 | 1 | 2:2 | 0.000 | 5 | 0 | 4:1 | 0.001 |
| OLSP40B17 | V4-2 | 0 | 2 | 2 | 1:3 | 0.003 | 1 | 2 | 1:2 | 0.260 |
| OLGI48J17 | V4-2 | 1 | 7 | 2 | 5:5 | 0.002 | 2 | 0 | 2:0 | 0.145 |
| OLSP42K18 | V6b-2 | 4 | 3 | 1 | 2:6 | 0.000 | 5 | 4 | 4:5 | 0.019 |
| OLKI20G22 | V10-2 | 0 | 1 | 0 | 1:0 | 0.081 | 3 | 0 | 0:3 | 0.699 |
| OLKI23P18 | V10-2 | 0 | 2 | 0 | 2:0 | 0.164 | 3 | 0 | 0:3 | 0.735 |
| OLKI35K02 | V10-2 | 0 | 1 | 0 | 1:0 | 0.081 | 3 | 0 | 0:3 | 0.699 |
| OLSP13L18 | V7-3 | 2 | 0 | 0 | 1:1 | 0.055 | 2 | 0 | 1:1 | 0.141 |
| OLKI53D04 | V10d-3 | 2 | 5 | 5 | 3:9 | 0.003 | 0 | 0 | 0:0 | 0.860 |
| OLKI61N09 | V11-3 | 4 | 2 | 2 | 3:5 | 0.045 | 1 | 0 | 1:0 | 0.512 |
| OLKI33H22 | V12-3 | 0 | 1 | 2 | 1:2 | 0.001 | 4 | 2 | 5:1 | 0.001 |
| OLKI30J18 | V13-3 | 0 | 0 | 2 | 2:0 | 0.774 | 0 | 0 | 0:0 | 0.576 |
a
Number of replacement (R) mutations vs. silent (S) mutations found in each VL subregion. The CDR3 and FR4 were not included in the analysis of mutations
b
PM value, probability calculated by multinomial distribution model that excess (for CDR) or scarcity (for FR) of mutations occurred by chance. The level of significance was P≤0.05