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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Genesis. 2014 May 2;52(7):657–670. doi: 10.1002/dvg.22781

Figure 7. Egfl7:eGFP+ cells contribute to neovasculature.

Figure 7

a) Aortic Ring assay. Aortae from Egfl7:eGFP transgenic adult mice embedded in collagen I gels for eight days develop eGFP+ endothelial sprouts from the quiescent aorta. Endogenous eGFP (green), anti-eGFP immunofluorescence (red) (scale bar = 100 μm). b) Enlargement of boxed area in panels in A (scale bar = 100 μm). c) Anti-eGFP immunofluorescence (green) and DyLight-594-conjugated isolectin B4 labeling (red) (scale bar = 100 μm). d) Matrigel implants. Day 10 Matrigel implants harvested from Egfl7:eGFP transgenic mice. Anti-eGFP immunofluorescence (green) and DyLight-594-conjugated isolectin B4 labeling (red) (scale bar = 20 μm).