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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Oncogene. 2012 Feb 13;32(2):151–159. doi: 10.1038/onc.2012.39

Figure 6.

Figure 6

Enforcing expression of IKBKE induces chemoresistance whereas knockdown of IKBKE sensitizes NSCLC cells to chemotherapy and reduces anti-apoptotic function of STAT3- and nicotine. (a) Expression of IKBKE renders cells resistance to chemotherapeutic drug-induced apoptosis. H661 cells expressing low endogenous IKBKE were transfected with IKBKE and then treated with the indicated agents (e.g., cisplatin 20 μM, gemcitabine 10μM and doxorubicin 1.0 μM) for 36 h (top panel). Apoptosis was assayed by PARP cleavage (panel 2) and TUNEL (bottom panel). (b) Depletion of IKBKE enhances apoptosis induced by chemotherapeutic drugs. IKBKE was knocked down in high-IKBKE H292 cells. After treatment with the indicated agents, the programmed cell death was evaluated by PARP cleavage and TUNEL assay for three times in triplicate. (c, d) STAT3- and nicotine-induced cell survival was largely abrogated by knockdown of IKBKE. A549 (c) and H1299 (d) cells were transfected and treated with the indicated plasmids and agents and then subjected to TUNEL assay. Single asterisk represents P<0.05 and double asterisks indicate P<0.01.