Abstract
Introduction
Hepatitis B surface antigen (HBsAg) level quantitation may be helpful for understanding the natural history of the disease and its response to treatment.
Aim
To determine the serum HBsAg levels during the different phases of chronic hepatitis B (CHB) infection and HBsAg levels of patients who are on treatment with oral antiviral drugs.
Material and methods
Patients were categorized in four groups: 9 patients in the immune clearance (IC) phase, 46 patients in the inactive carrier (INC) phase, 25 patients in the reactivated HBeAg negative disease (END) phase and 60 HBeAg negative patients who were on treatment with oral antiviral drugs. HBsAg levels were compared between all groups. HBsAg and HBV DNA levels were compared in three phases of HBV infection. Patients on treatment were divided into two groups, taking lamivudine (L) and taking tenofovir (T). HBsAg levels were compared between END, L and T groups.
Results
The HBsAg levels were different between each phase of CHB (p < 0.0001). HBsAg levels were highest in the IC phase and lowest in the INC phase. The HBsAg/HBV DNA ratio was significantly higher in the INC phase than the END and IC phases (p < 0.0001). HBsAg levels were higher in the END phase than the INC phase (p < 0.0001) and higher than the treatment group (p = 0.007). The HBsAg levels had a good correlation with HBV DNA in the natural course of CHB (r = 0.72, p < 0.0001). HBsAg levels were higher in the END group than the L and T groups (p < 0.05). HBsAg levels were higher in the L than the T group (p < 0.05).
Conclusions
This study demonstrates that HBsAg levels vary during the natural history of chronic hepatitis B infection. Also, the monitoring of HBsAg levels may help us to determine the best management strategy and to decide future treatment algorithms.
Keywords: chronic hepatitis B, hepatitis B surface antigen levels
Introduction
Hepatitis B surface antigen (HBsAg) was the first discovered hepatitis B virus (HBV) protein and the detection of HBsAg is the cornerstone of hepatitis B infection diagnosis [1]. Recent data from clinical trials have shown that quantitation of HBsAg levels may be helpful for understanding the natural history of the disease and its response to treatment.
The natural history of chronic hepatitis B (CHB) is divided into four different phases: the immune-tolerant phase, the immune-clearance phase (IC), inactive carrier (INC) and reactivated HBeAg negative disease (END) [2]. Several cross-sectional studies have compared HBsAg and HBV DNA levels during different phases of CHB. Both HBsAg and HBV DNA levels vary during the natural course of the infection. HBsAg and HBV DNA levels were highest during the immunotolerance phase and lowest in the inactive carrier phase [3, 4]. The HBeAg-negative patients have higher HBsAg and HBV DNA levels than inactive carriers [5]. A cutoff level lower than 2000 IU/ml HBV DNA is used to define the inactive carrier phase of hepatitis B infection but this cutoff level is still doubtful. Inactive carriers and END may be classified better by using cutoff levels of HBsAg and HBV DNA together. Inactive carriers can be classified with 94% to 100% accuracy by using a 1 to 2 × 103 IU/ml cutoff level for HBsAg and a 2 × 103 IU/ml cutoff level for HBV DNA together [6, 7].
HBsAg seroconversion is the ultimate goal of HBV infection therapy [2]. One important indicator of viral persistence is covalently closed circular DNA (cccDNA). cccDNA is considered as a surrogate marker of infected cells, which serves as the template for viral replication inside hepatocytes [8]. Reduction in cccDNA level after antiviral therapy is associated with a sustained virologic response [9]. Good correlations have been found between the absolute levels as well as the changes of serum HBsAg and cccDNA before and after antiviral therapy [10].
HBsAg quantitation may be a promising prognostic marker during the natural history of HBV infection and antiviral therapy.
Aim
We aimed to determine the serum HBsAg levels during the different phases of the natural history of CHB and also HBsAg levels of patients who are on treatment with oral antiviral drugs. We also aimed to investigate the correlation between HBsAg and HBV DNA levels.
Material and methods
One hundred and forty patients with chronic hepatitis B infection were included in this prospective study. The patients were recruited in İzmir Atatürk training and research hospital. No individuals were included who had hepatitis delta virus, hepatitis C virus or human immunodeficiency virus coinfection, acute infection with EBV or CMV, alcohol consumption (> 20 g/day), immunosuppressive drug use or malignant comorbidities. Patients with autoimmune hepatitis were excluded from this study.
Patients were classified into a phase of CHB according to the recently published European Association for the Study of the Liver (EASL) clinical practice guidelines [2].
Patients were categorized in four groups. Three different phases of CHB infection were included in the study: 9 patients in the IC phase (positive HBsAg for at least 6 months, positive HBeAg, persistent elevation of ALT, HBV DNA > 20 000 IU/ml), 46 patients in INC phase (positive HBsAg for at least 6 months, positive anti-HBeAg antibody, normal ALT levels more than two times in a year follow-up, HBV DNA < 2 000 IU/ml), 25 patients in END phase (positive HBsAg for at least 6 months, positive anti-HBeAg antibody, persistent elevation of ALT, HBV DNA > 20 000 IU/ml). Also, 60 HBeAg negative patients who were on treatment with oral antiviral drugs and had negative HBV DNA over one year were included in the treatment group. HBsAg levels were compared between all groups. The correlations between HBsAg and HBV DNA levels were determined in the natural course of HBV infection. Also, patients on treatment were divided into two groups, taking lamivudine (L) and taking tenofovir (T). HBsAg levels were compared between the END group, L group (n = 30) and T group (n = 21). Informed consent was obtained from all patients. The study was approved by the local Ethics Committees for Medical Research in accordance with the 1975 Declaration of Helsinki. Serum samples were stored at –70°C until use.
Quantitative serum HBsAg assay
Serum HBsAg levels were quantified using the Roche Elecsys HBsAg II Quant assay (Roche Diagnostics, Mannheim, Germany). Quantitative HBsAg levels were reported in IU/ml with a dynamic range of 0.05–52.000 IU/ml.
HBV DNA measurement
Serum HBV DNA was measured using an Abbott real-time HBV assay (Abbott Diagnostics, Santa Clara, CA) with a detection limit of 10–1 000 000 000 pg/ml (1 pg/ml = 3.4 copies/ml).
Statistical analysis
The Kolmogorov-Smirnov test was used to assess the normality of the data. Comparisons between the two groups were made using the Mann-Whitney U-test or independent sample T-test according to the distribution of the data. Comparisons between more than two groups were made using the Kruskal-Wallis test or ANOVA according to the distribution of the data. Correlations between groups were evaluated with Pearson's correlation test. A level of p less than 0.05 was considered statistically significant. All analyses were two-tailed and conducted using the computer-based statistics software program SPSS version 17.0 (SPSS Inc., Chicago, IL, USA).
Results
Patients in the IC phase were younger than patients in the INC (p < 0.01) or END phase (p < 0.01). HBsAg levels were different between each phase of CHB (between treatment-END groups; p = 0.007, treatment-INC groups; p < 0.0001, treatment-IC groups; p < 0.0001, END-INC groups; p < 0.0001, END-IC groups; p = 0.02, INC-IC groups; p < 0.0001). HBsAg levels were highest in the IC phase and lowest in the INC phase. The HBsAg/HBV DNA ratio was significantly higher in the INC phase than END (p < 0.0001) and IC phases (p < 0.0001) (Table I). HBsAg levels were higher in the END phase than the INC phase (p < 0.0001) and also higher than the treatment group (p = 0.007). HBsAg levels had a good correlation with HBV DNA in the natural course of CHB (r = 0.72, p < 0.0001) (Figure 1). HBsAg levels were compared between the END group, L group and T group. HBsAg levels were higher in the END group than the L group (p < 0.01) and T group (p < 0.01). HBsAg levels were higher in the L group than the T group (p < 0.01) (Table II).
Table I.
Comparison between groups with regard to age, sex, ALT, HBsAg and HBV DNA levels
| Variable | Treatment group (n = 60) |
END phase (n = 25) |
INC phase (n = 46) |
IC phase (n = 9) |
Value of p |
|---|---|---|---|---|---|
| HBeAg status | (–) | (–) | (–) | (+) | |
| HBsAg levels | 5490 ±7180 | 8360 ±6562 | 2456 ±4055 | 59097 ±55093 | < 0.0001 |
| HBsAg log | 3.43 ±0.59 | 3.79 ±0.41 | 2.78 ±0.84 | 4.50 ±0.59 | < 0.0001 |
| HBV DNA | 13.9 ±44.9 | 0.9 × 107 ±2.5 × 107 | 912 ±1212 | 6.2 × 108 ±7.5 × 108 | < 0.0001 |
| HBV DNA log10 | 1.77 ±0.38 | 5.70 ±1.27 | 2.49 ±0.75 | 8.28 ±0.85 | < 0.0001 |
| HBsAg log/HBV DNA log10 | 1.88 ±0.61 | 0.69 ±0.16 | 1.18 ±0.42 | 0.55 ±0.06 | < 0.0001 |
| Age [year] | 47.8 ±13.0 | 39.3 ±10.8 | 45.3 ±11.4 | 35.3 ±16.1 | 0.004 |
| Gender (female/male) | 24/36 | 13/12 | 24/22 | 5/4 | > 0.05 |
| ALT [IU/ml] | 26.5 ±10.9 | 89 ±123 | 27.6 ±11.2 | 124 ±122 | < 0.0001 |
END phase – HBeAg negative disease phase, INC phase – inactive carrier phase, IC phase – immune clearance phase
Figure 1.
Relationship between HBsAg and HBV DNA levels during the CHB infection
Table II.
Comparison of HBsAg levels among the END, lamivudine and tenofovir groups
| Variable | Lamivudine group (n = 30) | Tenofovir group (n = 21) | END group (n = 25) | Value of p |
|---|---|---|---|---|
| HBsAg levels | 6224 ±7996 | 3895 ±6300 | 8630 ±6562 | < 0.01 |
| HBsAg log | 3.46 ±0.66 | 3.27 ±0.56 | 3.79 ±0.41 | < 0.01 |
| Age | 46.6 ±13.7 | 50.0 ±11.1 | 39.3 ±10.8 | < 0.01 |
| Gender (F/M) | 15/15 | 6/15 | 13/12 | > 0.05 |
| ALT [IU/ml] | 26.0 ±12.0 | 26.1 ±8.4 | 89.0 ±123 | < 0.0001 |
Discussion
The natural history of CHB is usually determined by using HBeAg status, ALT and HBV DNA levels [11]. There are limited data about the hepatitis B surface antigen levels in the natural history of CHB. In our study we found that HBsAg and HBV DNA levels differ during the natural history of chronic hepatitis B infection in parallel to each other. The INC phase was characterized by the highest HBsAg/HBV DNA ratio. Also the correlation of HBsAg and HBV DNA during the natural course of CHB was an interesting observation. These results suggest that HBsAg quantitation, combined with HBV DNA, may be useful for better determination of the phases of CHB infection.
Quantitative serum HBsAg was a good surrogate marker for both cccDNA and total intrahepatic HBV DNA [10]. Recent studies have suggested that the quantitation of HBsAg in HBeAg negative HBV infection or HBV/hepatitis delta virus dual infection has potential for monitoring the response to interferon and oral antiviral maintenance therapy [12, 13].
In interferon-based therapeutic regimens, the early drop in serum HBsAg levels has a high predictive value for a sustained virological response in both HBeAg-positive and HBeAg-negative patients [14, 15]. However, limited data are available on the potential usefulness of HBsAg titer monitoring during long-term NA treatment. Oral antiviral drugs may decrease the HBsAg levels [13, 16, 17]. We found that HBsAg levels are lower in HBeAg negative patients who are treated with an oral antiviral drug than treatment-naive HBeAg negative patients. Also, HBsAg titers are lower in tenofovir-treated patients than lamivudine-treated patients.
Conclusions
This study demonstrates that HBsAg levels differ during the natural history of chronic hepatitis B infection and HBsAg levels are associated with HBV DNA levels. These findings may help us to understand the natural course of hepatitis B infection, as an example for identification of true inactive carriers. Also, the monitoring of HBsAg levels may help us to determine the best management strategy and HBsAg quantification may help us to decide future treatment algorithms for both immune-modulator therapy and oral nucleos(t)ide analogue therapy.
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