Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem Cells

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(Stem Cell Reports 2, 838–852, June 3, 2014)

An inadvertent production error occurred after the proof stage while setting Table 1 for print. As a result, the labels “GFP Negative,” “GFP Positive,” and “Total” were positioned incorrectly. We apologize for this mistake and provide a corrected version of Table 1 below. The online version has also been corrected.

Table 1.

WIBR3-hESC Derived: Teratoma-derived organoidsa
Teratomas	GFP Negative			GFP Positive			Total
	No. of organoids formed	No. of cells sorted	Cells forming organoids (%)	No. of organoids formed	No. of cells sorted	Cells forming organoids (%)	No. of organoids formed	No. of cells sorted	Cells forming organoids (%)
Lgr5-GFP N-term	0	1,000	0	23	1,000	2.3	∼50	>50,000	<0.1%
Lgr5-GFP N-term	0	1,710	0	101	1,710	5.91	∼50	>50,000	<0.1%
Lgr5-GFP N-term	0	1,675	0	21	1,675	1.25	∼50	>50,000	<0.1%
Lgr5-GFP C-term	0	4,203	0	38	4,203	0.9	∼50	>50,000	<0.1%
Lgr5-GFP C-term	0	6,613	0	81	6,613	1.22	∼50	>50,000	<0.1%
Lgr5-GFP N-term	0	6,046	0	3	6,046	0.05	∼50	>50,000	<0.1%
Organoid-rederived organoidsb
Organoids	GFP Negative			GFP Positive			Total
No. of organoids formed	No. of cells sorted	Cells forming organoids (%)	No. of organoids formed	No. of cells sorted	Cells forming organoids (%)	No. of organoids formed	No. of cells sorted	Cells forming organoids (%)
Lgr5-GFP N-term	0	1,500	0	7	1,500	0.47	7	15,000	0.05
Lgr5-GFP N-term	24	6,700	0.36	140	6,700	2.09	65	13,000	0.5
Lgr5-GFP N-term	3	7,960	0.04	20	7,960	0.26	15	38,450	0.04

^aSix independent teratomas FACS sorted and assayed for organoid formation.

^bThree organoids derived from three independent unsorted teratomas (dissociated, FACs sorted, and assayed for organoid formation).