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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Chembiochem. 2014 Jul 7;15(10):1418–1421. doi: 10.1002/cbic.201402119

Table 1.

Kinetic parameters for CalO6 using variable SNAC-OSA (0.1 - 5 mM) and SAM (0.1 - 4 mM) in 25 mM Tris, 5 mM MgCl2, pH 8.0 at 35 °C.

Product Constant
Substrate		 Varied
Substrate		 kcat × 10−3
(min−1)		 KM (mM) kcat / KM × 10−3
(mM−1min−1)
P1 SAM SNAC-OSA 1.03 ± 0.03 1.27 ± 0.08 0.81
SNAC-OSA SAM 0.94 ± 0.03 0.30 ± 0.03 3.13
P2 SAM SNAC-OSA 0.33 ± 0.01 1.31 ± 0.11 0.25
SNAC-OSA SAM 0.36 ± 0.01 0.56 ± 0.06 0.64
Total SAM SNAC-OSA 1.35 ± 0.03 1.27 ± 0.07 1.06
SNAC-OSA SAM 1.29 ± 0.03 0.35 ± 0.04 3.69