Figure 2. KSHV vFLIP-induced G1 arrest is NF-κB-dependent.
(A) HeLa-G and its ΔN-I-κBα-expressing derivative, ΔN-I-κBαN4, were transduced with LV-vFLIP-puro and selected with puromycin as in Fig. 1. Cells were photographed at day 2 and 5 after puromycin selection. (B) Cells transduced with LV-puro or LV-vFLIP-puro were harvested four days after puromycin selection and analyzed by immunoblotting using the indicated antibodies.