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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Oncogene. 2014 Jan 27;34(4):496–505. doi: 10.1038/onc.2013.567

Figure 4. Characterizations of HeLa cells stably expressing KSHV vCyclin.

Figure 4

(A) Phase contrast photographs of HeLa–G and its derivative, vCyc-G2, that expresses dual Flag-tagged- (2Flag-) vCyclin,. (B) Immunoblot analysis of HeLa–G and vCyc-G2 using a Flag-epitope antibody. (C) Flow cytometry histograms of HeLa-G and vCyc-G2. Exponentially growing HeLa-G and vCyc-G2 cells were subjected to flow cytometry as described in Materials and Methods. The percentage of cells in G1, S, and G2/M respectively was determined as in Fig. 1C.