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. 1978 Jan;75(1):451–453. doi: 10.1073/pnas.75.1.451

Increased turnover of arterial collagen in hypertensive rats.

R Nissen, G J Cardinale, S Udenfriend
PMCID: PMC411267  PMID: 272662

Abstract

The turnover of total collagen in several tissues of 12-week-old normotensive and hypertensive rats was estimated by using tritium-labeled proline as a precursor. The effect of reutilization of the label was minimized by treatment with large doses of unlabeled proline subsequent to administering the radioactive imino acid. The collagen from skin, tail tendon, aorta, and mesenteric artery in normotensive animals had a half-life of about 60--70 days. In hypertensive animals the half-lives of skin and tail tendon collagen were unchanged but the half-lives of collagen in the aorta and mesenteric artery were reduced to 17 days.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GERBER G., GERBER G., ALTMAN K. I. Studies on the metabolism of tissue proteins. I. Turnover of collagen labeled with proline-U-C14 in young rats. J Biol Chem. 1960 Sep;235:2653–2656. [PubMed] [Google Scholar]
  2. Halme J., Uitto J., Kahanpä K., Karhunen P., Lindy S. Protocollagen proline hydroxylase activity in experimental pulmonary fibrosis of rats. J Lab Clin Med. 1970 Apr;75(4):535–541. [PubMed] [Google Scholar]
  3. Iwatsuki K., Cardinale G. J., Spector S., Udenfriend S. Hypertension: increase of collagen biosynthesis in arteries but not in veins. Science. 1977 Oct 28;198(4315):403–405. doi: 10.1126/science.198877. [DOI] [PubMed] [Google Scholar]
  4. Iwatsuki K., Cardinale G. J., Spector S., Udenfriend S. Reduction of blood pressure and vascular collagen in hypertensive rats by beta-aminopropionitrile. Proc Natl Acad Sci U S A. 1977 Jan;74(1):360–362. doi: 10.1073/pnas.74.1.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jackson S. H., Heininger J. A. A study of collagen reutilization using an 18O2 labeling technique. Clin Chim Acta. 1974 Mar 15;51(2):163–171. doi: 10.1016/0009-8981(74)90026-6. [DOI] [PubMed] [Google Scholar]
  6. KAO K. Y., HILKER D. M., MCGAVACK T. H. Connective tissue V. Comparison of synthesis and turnover of collagen and elastin in tissues of rat at several ages. Proc Soc Exp Biol Med. 1961 Feb;106:335–338. doi: 10.3181/00379727-106-26329. [DOI] [PubMed] [Google Scholar]
  7. KAO K. Y., HILKER D. M., MCGAVACK T. H. Connective tissue. IV. Synthesis and turnover of proteins in tissues of rats. Proc Soc Exp Biol Med. 1961 Jan;106:121–124. doi: 10.3181/00379727-106-26257. [DOI] [PubMed] [Google Scholar]
  8. Kivirikko K. I., Laitinen O., Prockop D. J. Modifications of a specific assay for hydroxyproline in urine. Anal Biochem. 1967 May;19(2):249–255. doi: 10.1016/0003-2697(67)90160-1. [DOI] [PubMed] [Google Scholar]
  9. Kivirikko K. I., Risteli L. Biosynthesis of collagen and its alterations in pathological states. Med Biol. 1976 Jun;54(3):159–186. [PubMed] [Google Scholar]
  10. Mussini E., Hutton J. J., Jr, Udenfriend S. Collagen proline hydroxylase in wound healing, granuloma formation, scurvy, and growth. Science. 1967 Aug 25;157(3791):927–929. doi: 10.1126/science.157.3791.927. [DOI] [PubMed] [Google Scholar]
  11. NEUBERGER A., PERRONE J. C., SLACK H. G. B. The relative metabolic inertia of tendon collagen in the rat. Biochem J. 1951 Jul;49(2):199–204. [PMC free article] [PubMed] [Google Scholar]
  12. NEUBERGER A., SLACK H. G. B. The metabolism of collagen from liver, bone, skin and tendon in the normal rat. Biochem J. 1953 Jan;53(1):47–52. doi: 10.1042/bj0530047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ooshima A., Fuller G. C., Cardinale G. J., Spector S., Udenfriend S. Increased collagen synthesis in blood vessels of hypertensive rats and its reversal by antihypertensive agents. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3019–3023. doi: 10.1073/pnas.71.8.3019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ooshima A., Fuller G. C., Cardinale G. J., Spector S., Udenfriend S. Reduction of collagen biosynthesis in blood vessels and other tissues by reserpine and hypophysectomy. Proc Natl Acad Sci U S A. 1977 Feb;74(2):777–779. doi: 10.1073/pnas.74.2.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ooshima A., Fuller G., Cardinale G., Spector S., Udenfriend S. Collagen biosynthesis in blood vessels of brain and other tissues of the hypertensive rat. Science. 1975 Nov 28;190(4217):898–900. doi: 10.1126/science.171771. [DOI] [PubMed] [Google Scholar]
  16. PETERKOFSKY B., PROCKOP D. J. A method for the simultaneous measurement of the radioactivity of proline-C14 and hydroxyproline-C14 in biological materials. Anal Biochem. 1962 Nov;4:400–406. doi: 10.1016/0003-2697(62)90141-0. [DOI] [PubMed] [Google Scholar]
  17. POPENOE E. A., VAN SLYKE D. D. The formation of collagen hydroxylysine. J Biol Chem. 1962 Nov;237:3491–3494. [PubMed] [Google Scholar]
  18. Poole B. The kinetics of disappearance of labeled leucine from the free leucine pool of rat liver and its effect on the apparent turnover of catalase and other hepatic proteins. J Biol Chem. 1971 Nov;246(21):6587–6591. [PubMed] [Google Scholar]
  19. Popper H., Uenfriend S. Hepatic fibrosis. Correlation of biochemical and morphologic investigations. Am J Med. 1970 Nov;49:707–721. doi: 10.1016/s0002-9343(70)80135-8. [DOI] [PubMed] [Google Scholar]
  20. REINER J. M. The study of metabolic turnover rates by means of isotopic tracers. I. Fundamental relations. Arch Biochem Biophys. 1953 Sep;46(1):53–79. doi: 10.1016/0003-9861(53)90170-2. [DOI] [PubMed] [Google Scholar]
  21. REINER J. M. The study of metabolic turnover rates by means of isotopic tracers. II. Turnover in a simple reaction system. Arch Biochem Biophys. 1953 Sep;46(1):80–99. doi: 10.1016/0003-9861(53)90171-4. [DOI] [PubMed] [Google Scholar]
  22. TROLL W., LINDSLEY J. A photometric method for the determination of proline. J Biol Chem. 1955 Aug;215(2):655–660. [PubMed] [Google Scholar]
  23. UDENFRIEND S., ZALTZMAN-NIRENBERG P. NOREPINEPHRINE AND 3,4DIHYDROXYPHENETHYLAMINE TURNOVER IN GUINEA PIG BRAIN IN VIVO. Science. 1963 Oct 18;142(3590):394–396. doi: 10.1126/science.142.3590.394. [DOI] [PubMed] [Google Scholar]
  24. de Champlain J., Krakoff L. R., Axelrod J. Catecholamine metabolism in experimental hypertension in the rat. Circ Res. 1967 Jan;20(1):136–145. doi: 10.1161/01.res.20.1.136. [DOI] [PubMed] [Google Scholar]

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